Evaluation of Isotope 32P Method to Mark Culex pipiens (Diptera: Culicidae) in a Laboratory

  • Chongxing Zhang Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People’s Republic of China
  • Guihong Shi Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People’s Republic of China Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medical University, Taian, Shandong 271000, China
  • Yuqiang Zhao Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People’s Republic of China
  • Dongmei Yan Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People’s Republic of China
  • Huaiju Li Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People’s Republic of China
  • Hongmei Liu Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People’s Republic of China
  • Itsanun Wiwatanaratanabutr Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand
  • Maoqing Gong Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People’s Republic of China
Keywords:
Radioactive isotope, Mark, Culex pipiens, Rabbit

Abstract

Background: The aim of the current study was to develop a marking technique as an internal marker to mark post blood meal mosquitoes by using stable phosphate isotope 32P and determine the optimal concentration of it.Methods: An isotonic physiological saline solution, containing different concentration of radioactive isotope 32P-labeled disodium phosphate (Na2H32PO4) was injected into rabbits via the jugular vein in the laboratory. Emerged Cx. pipiens were marked after feeding on rabbit. At the same time, the labeled conditions of emerged Cx. pipiens were also measured by placing feces of No. 6 rabbit into containers with mosquito larvae and pupae inside.Results: According to the label condition of Cx. pipiens after taking blood and the effect of different dosage Na2H32PO4 on rabbit health, the optimal concentration of radioactive isotope was determined, that is, 0.1211 mCi/kg. By placing feces of No. 6 rabbit into containers with mosquito larvae and pupae inside, the emerged mosquitoes were also labeled. Therefore, feeding mosquitoes on the animal injected with radioactive Na2H32PO4 was more prac­tical for detecting and tracing mosquitoes.Conclusion: The method was less time-consuming, more sensitive and safer. This marking method will facilitate post-bloodmeal studies of mosquitoes and other blood-sucking insects.

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Published
2016-05-05
How to Cite
1.
Zhang C, Shi G, Zhao Y, Yan D, Li H, Liu H, Wiwatanaratanabutr I, Gong M. Evaluation of Isotope 32P Method to Mark Culex pipiens (Diptera: Culicidae) in a Laboratory. J Arthropod Borne Dis. 10(2):211-221.
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Original Article