Molecular Characterization of Paederus Spp (Coleoptera: Staphylinidae, Paederinae) the Agent of Human Linear Dermatitis in the Caspian Sea Coast, North of Iran
-
Abbas Heydari
,
Sinan Anlaş
,
Hasan Bakhshi
,
Mona Koosha
,
Nayyereh Choubdar
,
Somayeh Panahi-Moghadam
,
Mohammad Ali Oshaghi
Abstract
Background: A combined morphological and molecular survey was performed to determine the agent of human linear dermatitis Paederus Fabricius, 1775 (Coleoptera: Staphylinidae, Paederinae) species composition in Mazandaran Province in the Caspian Sea coast in northern Iran, where most of linear dermatitis cases of the country occurred.
Methods: Altogether, 397 Paederus specimens were collected from May to August 2021 and classified using morphological characters and ITS2-rDNA sequence analysis.
Results: Morphological investigation revealed that all the specimens were Paederus fuscipes. ITS2 polymerase chain reaction (PCR) direct-sequences and the profiles of restriction fragment length polymorphism (RFLP) derived from digestion of PCR products by HinfI, HpaII, and SalI enzymes were identical confirming the morphological results, implying that all specimens belonged to a single taxon.
Conclusion: Paederus fuscipes (Fabricius, 1775) is considered the dominant taxon and responsible for linear dermatitis in Mazandaran Province. To our knowledge, we have provided the first molecular typing of Paederus beetles at the species level, suggesting that ITS2-rDNA characterization is an alternative tool for species discrimination of Paederus spp.
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5. Frank JH, Thomas MC (2016) Rove bee¬tles of the world, Staphylinidae (Insecta: Coleoptera: Staphylini¬dae). EENY. 114: 1–8.
6. Echegaray ER, Cloyd RA (2013) Life his-tory characteristics of the rove bee¬tle, Dalotia coriaria (Coleoptera: Staph¬ylin¬i-dae) under laboratory conditions. J Kans Entomol Soc. 86: 145–154.
7. Frank JH, Kanamitsu K (1987) "Paederus, sensu lato (Coleoptera: Staphylinidae): natural history and medical importance". J Med Entomol. 24: 155–191.
8. Pavan M, Bo G (1953) Pederin, toxic prin-ciples obtained in the crystalline state from the beetle Paederus fuscipes. Curt Physiol comp Oecologia. 3: 307‒312.
9. Mullen GR, Durden LA (2009) Medical and Veterinary Entomology. Elsevier Aca-demic Press Inc, London.
10. Jacobs-Lorena M, Brega, Baglioni AC (1971) Inhibition of protein synthesis in reticulocytes by antibiotics. V. Mecha-nism of action of pederine, an inhibitor of initiation and elongation. Biochim Biophys Acta. 240: 263–272.
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14. Kellner RLL, Dettner K (1995) Allocation of pederin during lifetime of Paederus rove beetles (Coleoptera: Staphylini¬dae): Evidence for polymorphism of hemo-lymph toxin. J Chem Ecol. 21: 1719–1733.
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2. Grebennikov VV, Newton AF (2009) Good-bye Scydmaenidae, or why the ant-like stone beetles should become mega¬diverse Staphylinidae sensu latis-simo (Coleoptera). Eur J Entomol. 106 (2): 275‒301.
3. Vieira JS, Ribeiro-Costa CS, Caron E (2014) Rove beetles of medical im-portance in Brazil (Coleoptera, Staph-ylinidae, Paeder¬inae). Rev Bras Ento-mol. 58: 244–260.
4. Herman LH (2001) Catalog of the Staph-ylinidae (Insecta: Coleoptera). 1758 to the end of the second millennium. Vol-umes I‒VII. Bull Am Mus Nat Hist. 265: 1‒4218.
5. Frank JH, Thomas MC (2016) Rove bee¬tles of the world, Staphylinidae (Insecta: Coleoptera: Staphylini¬dae). EENY. 114: 1–8.
6. Echegaray ER, Cloyd RA (2013) Life his-tory characteristics of the rove bee¬tle, Dalotia coriaria (Coleoptera: Staph¬ylin¬i-dae) under laboratory conditions. J Kans Entomol Soc. 86: 145–154.
7. Frank JH, Kanamitsu K (1987) "Paederus, sensu lato (Coleoptera: Staphylinidae): natural history and medical importance". J Med Entomol. 24: 155–191.
8. Pavan M, Bo G (1953) Pederin, toxic prin-ciples obtained in the crystalline state from the beetle Paederus fuscipes. Curt Physiol comp Oecologia. 3: 307‒312.
9. Mullen GR, Durden LA (2009) Medical and Veterinary Entomology. Elsevier Aca-demic Press Inc, London.
10. Jacobs-Lorena M, Brega, Baglioni AC (1971) Inhibition of protein synthesis in reticulocytes by antibiotics. V. Mecha-nism of action of pederine, an inhibitor of initiation and elongation. Biochim Biophys Acta. 240: 263–272.
11. Burres NS, Clement JJ (1989) Antitumor activity and mechanism of action of the novel marine natural products my-calamide-A and -B and onnamide. Can-cer Res. 49: 2935–2940.
12. Perry NB, Blunt JW, Munro MHG, Thomp¬son AM (1990) Antiviral and an-titumor agents from a New Zealand sponge, Mycale sp. 2. Structures and so-lution conformations of mycalamides A and B. J Org Chem. 55: 223–227.
13. Piel J (2002) Polyketide synthesis-peptide synthetase gene cluster from an uncul-tured bacterial symbiont of Paederus bee¬tles. Proc Natl Acad Sci U S A. 99(22): 14002–14007.
14. Kellner RLL, Dettner K (1995) Allocation of pederin during lifetime of Paederus rove beetles (Coleoptera: Staphylini¬dae): Evidence for polymorphism of hemo-lymph toxin. J Chem Ecol. 21: 1719–1733.
15. Kellner RLL (2002) Molecular identifica-tion of an endosymbiotic bacterium as-sociated with pederin biosynthesis in Pae¬derus sabaeus (Coleoptera: Staph-ylini¬dae). Insect Biochem Mol Biol. 32: 389–395.
16. Piel J, Höfer I, Hui D (2004) Evidence for a Symbiosis Island Involved in Horizon-tal Acquisition of Pederin Biosynthetic Capabilities by the Bacterial Symbiont of Paederus fuscipes Beetles. J Bacteri-ol. 186: 1280–1286.
17. Maleki-Ravasan N, Akhavan N, Raz A, Jafari M, Zakeri S, Dinparast Djadid N (2018) Co-occurrence of pederin-produc¬ing and Wolbachia endobacteria in Paederus fuscipes Curtis, 1840 (Col-eoptera: Staphilinidae) and its evolu-tionary conse¬quences. Microbiology-open. 8(7): e00777.
18. Chamankar B, Maleki-Ravasan N, Karami M, Forouzan E, Karimian F, Naeimi S, Choobdar N (2023) The structure and di¬versity of microbial communities in Pae¬derus fuscipes (Coleoptera: Staph-ylini¬dae): from ecological paradigm to patho¬biome. Microbiome. 11(1): 11.
19. Haine ER (2008) Symbiont-mediated pro-tection. Proc Biol Sci. 275: 353‒361.
20. Coiffait H (1982) Coléoptères Staphylini-dae de la région Paléarctique occi-dentale. IV. Sous-famille Pederinae. Tri-bus Pederini 1 (Paederi, Lathrobii). Nouv Rev En¬tomol. 12(4): 1–440.
21. Li XY, Zhou HZ (2009) A review of Chi-nese species of the subgenus Paederus s. str. (Coleop-tera: Staphylinidae: Paeder-inae) with description of a new species. Zootaxa. 2083: 46‒64.
22. Nikbakhtzadeh MR, Nader Mi, Safa P (2012) Faunal diversity of Paederus Fab¬ricius, 1775 (Coleoptera: Staphylini-dae) in Iran. Insecta Mundi. 267: 1–9.
23. Hill SM, Crampton JM (1994) DNA-based methods for the identification of insect vectors. Ann Trop Med Parasitol. 88: 227–250.
24. Collins FH, Paskewitz SM (1996) A re-view of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species. Insect Mol Biol. 5: 1–9.
25. Karimian F, Oshaghi MA, Sedaghat MM, Waterhouse RM, Vatandoost H, Hanafi-Bojd AA, Ravasan NM, Chavshin AR (2014) Phylogenetic analysis of the ori-ental-Palearctic-Afrotropical members of Anopheles (Culicidae: Diptera) based on nuclear rDNA and mitochondrial DNA characteristics. Jpn J Infect Dis. 67(5): 361–367.
26. Koosha M, Oshaghi MA, Sedaghat MM, Vatandoost H, Azari-Hamidian S, Abai MR, Hanafi-Bojd AA, Mohtarami F (2017) Sequence analysis of mtDNA COI barcode region revealed three hap-lo¬types within Culex pipiens assem¬blage. Exp Parasitol. 181: 102–110.
27. Oshaghi MA, Yaghobi-Ershadi MR, Shemshad K, Pedram M, Amani H (2008) The Anopheles superpictus com-plex: introduction of a new malaria vec-tor complex in Iran. Bull Soc Pathol Ex-ot. 101 (5): 429–434.
28. Jafari S, Oshaghi MA, Akbarzadeh K, Abai MR, Koosha M, Mohtarami F (2019) Identification of Forensically Important Flesh Flies Using the Cyto-chrome C Ox¬idase Subunits I and II Genes. J Med Entomol. 56(5): 1253–1259.
29. Khanzadeh F, Khaghaninia S, Maleki-Rav¬asan N, Oshaghi MA, Adler PH (2020) Black flies (Diptera: Simuliidae) of the Aras River Basin: Species com-position and floral visitation. Acta Trop. 209: 105536.
30. Talebzadeh F, Oshaghi MA, Akbarzadeh K, Panahi-Moghadam S (2020) Molecu-lar Species Identification of Six Forensi-cally Important Iranian Flesh Flies (Dip-tera). J Arthropod Borne Dis. 14(4): 416–424.
31. Gómez-Zurita J, Juan C, Petitpierre E (2009) Sequence, secondary structure and phylogenetic analyses of the ribo-somal inter¬nal transcribed spacer 2 (ITS2) in the Timarcha leaf beetles (Coleoptera: Chrysome¬lidae). Insect Mol Biol. 9: 591–604.
32. Gallego D, Galián J (2001) The internal transcribed spacers (ITS1 and ITS2) of the rDNA differentiates the bark beetle forest pests Tomicus destruens and T. pin¬iperda. Insect Mol Biol. 10: 415–420.
33. Dezfouli SR, Oshaghi MA, Vatandoost H, Assmar M (2003) rDNA-ITS2 based spe¬cies-diagnostic polymerase chain re-ac¬tion assay for identification of sibling species of Anopheles fluviatilis in Iran. Southeast Asian J Trop Med Public Health. 34 Suppl 2: 56–60.
34. Bologna MA, Oliverio M, Pitzalis M, Mariottini P (2008) Phylogeny and evo-lutionary history of the blister beetles (Coleoptera, Meloidae). Mol Phylogene. Evol. 48: 679–693.
35. Trizzino M, Audisio P, Antonini G, De Biase A, Mancini E (2009) Comparative analysis of sequences and secondary struc¬tures of the rRNA internal tran-scribed spacer 2 (ITS2) in pollen beetles of the subfamily Meligethinae (Coleop-tera, Nitidulidae): potential use of slip-page-de¬rived sequences in molecular systematics. Mol Phylogenet Evol. 51: 215–226.
36. Germain JF, Chatot C, Meusnier I, Artige E, Rasplus JY, Cruaud A (2013) Molec-ular identification of Epitrix potato flea beetles (Coleoptera: Chrysomelidae) in Europe and North America. Bull Ento-mol Res. 1: 1–9.
37. Young I, Coleman AW (2004) The ad-vantages of the ITS2 region of the nu-clear rDNA cistron for analysis of phy-logenetic relationships of insects: a Dro-sophila example. Mol Phylogenet Evol. 30: 236–242.
38. Linton YM, Samanidou-Voyadjoglou A, Harbach RE (2002) Ribosomal ITS2 se-quence data for Anopheles maculipennis and An. messeae in northern Greece, with a critical assessment of previously published sequences. Insect Mol Biol. 11: 379–383.
39. Moin-Vaziri V, Oshaghi MA, Yaghoobi-Ershadi MR, Derakhshandeh-Peykar P, Abai MR, Mohtarami F, Zahraei-Rameza¬ni AR, Nadim A (2016) ITS2-rDNA Sequence Variation of Phleboto-mus sergenti s.l. (Dip: Psychodidae) Populations in Iran. J Arthropod Borne Dis. 10(4): 462–473.
40. Janbakhsh B, Ardalan A (1977) Rove bee-tles (Coleoptera: Staphylinidae) and their medical importance. Iranian J Pub-lic Health. 6(2): 70–77.
41. Majidi-Shad B, Janbakhsh B, Bagheri-Ze-nooz B (1989) The species of genus Pae-derus (Col. Staphylinidae) that caused hu¬man dermatitis in the southern region of Caspian Sea. The 9th Plant Protection Con¬gress of Iran, 1989, Iranian Society of Plant Protection, Mashhad, Iran, p. 40.
42. Nikbakhtzadeh MR, Tirgari S (2008) Medically Important Beetles of Iran. J Ven-om Anim Toxins incl Trop Dis. 14: 597–618.
43. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct se-quencing of fungal ribosomal RNA genes for phylogenetics. In: M.A. Innis, D. H. Gelfand, J.J. Sninsky, and T.J. White (eds), PCR Pro¬tocols: A guide to methods and applica¬tions, Academic Press, Inc., New York, pp. 315–322.
44. Vincze T, Posfai J, Roberts RJ (2003) NEBcutter: A program to cleave DNA with restriction enzymes. Nucleic Acids Res. 31: 3688–3691.
45. Bohác J (1985) Review of the subfamily Pederinae (Coleoptera Staphylinidae) in Czechoslovakia. Acta Entomol Bohe-mos. 82: 360–385.
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| Files | ||
| Issue | Vol 17 No 1 (2023) | |
| Section | Original Article | |
| DOI | https://doi.org/10.18502/jad.v17i1.13206 | |
| Keywords | ||
| Paederus fuscipes; Rove beetle; Linear dermatitis; ITS2-rDNA; Iran | ||
| Rights and permissions | |
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Heydari A, Anlaş S, Bakhshi H, Koosha M, Choubdar N, Panahi-Moghadam S, Oshaghi MA. Molecular Characterization of Paederus Spp (Coleoptera: Staphylinidae, Paederinae) the Agent of Human Linear Dermatitis in the Caspian Sea Coast, North of Iran. J Arthropod Borne Dis. 2023;17(1):94–104.
