Differential Larval Toxicity and Oviposition Altering Activity of Some Indigenous Plant Extracts against Dengue and Chikungunya Vector Aedes albopictus.

  • Ruchi Yadav Mail Vector Management Division, Defence Research and Development Establishment, Gwalior, India.
  • Varun Tyagi Vector Management Division, Defence Research and Development Establishment, Gwalior, India.
  • Sachin N Tikar Vector Management Division, Defence Research and Development Establishment, Gwalior, India.
  • Ajay K Sharma Vector Management Division, Defence Research and Development Establishment, Gwalior, India.
  • Murlidhar J Mendki Vector Management Division, Defence Research and Development Establishment, Gwalior, India.
  • Ashok K Jain School of Studies Botany, Jiwaji University, Gwalior, India.
  • Devanathan Sukumaran Vector Management Division, Defence Research and Development Establishment, Gwalior, India.
Aedes albopictus, Larvicide, Oviposition activity, Plant extracts, Vector Management


Background: Mosquitoes are well known as vectors of several disease causing pathogens. The extensive use of synthetic insecticides in the mosquito control strategies resulted to the development of pesticide resistance and fos- tered environmental deterioration. Hence in recent years plants become alternative source of mosquito control agents. The present study assessed the larvicidal and oviposition altering activity of six different plants species- Alstonia scholaris, Callistemon viminalis, Hyptis suaveolens, Malvastrum coromandelianum, Prosopis juliflora, Vernonia cinerea against Aedes albopictus mosquito in laboratory.Methods: Leaf extracts of all the six plants species in five different solvents of various polarities were used in the range of 20–400ppm for larval bioassay and 50,100 and 200ppm for cage bioassay (for the study of oviposition be- havior) against Ae. albopictus. The larval mortality data were recorded after 24 h and subjected to Probit analysis to determine the lethal concentrations (LC50), while OAI (Oviposition activity index) was calculated for oviposition altering activity of the plant extracts.Results: Vernonia cinerea extract in acetone and C. viminalis extract in isopropanol were highly effective against Aedes albopictus larvae with LC50 value 64.57, 71.34ppm respectively. Acetone extract of P.  juliflora found to be strong oviposition-deterrent which inhibited >2 fold egg laying (OAI-0.466) at 100ppm.Conclusion: Vernonia cinerea and C. viminallis leaf extracts have the potential to be used as larvicide and P.juliflora as an oviposition-deterrent for the control of Ae. albopictus mosquito.


Abbott SW (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol. 18: 265–267.

Amusan AAS, Idowu AB, Arowolo FS (2005) Comparative toxicity of bush tea leaves (Hyptis suaveolens) and orange peel (Citrus sinensis) oil extract on larvae of the yellow fever mosquito Aedes aegypti. Tanzan Health Res Bull. 7(3): 174–178.

Ameen M-ul, Shahjahan RM, Khan HR, Chowdhury AKA (1985) Larvicidal effect of indigenous Derris elliptica root on Aedes aegypti (Diptera: Culicidae). Int J Entomol. 1: 39–43.

Arivoli S, Tennyson S, Martin JJ (2011) Larvicidal efficacy of Vernonia cinerea (L.) (Asteraceae) leaf extracts against the filarial vector Culex quinquefasciatus Say (Diptera: Culicidae). J Biopest. 4:37–42.

Bakar AA, Sulaiman S, Omar B, Ali RM (2012) Evaluation of Melaleuca cajuputi (Family: Myrtaceae) Essential Oil in Aerosol Spray Cans against Dengue Vectors in Low Cost Housing Flats. J Arthropod-Borne Dis. 6(1): 28–35.

Bansal SK, Karam SV, Sharma S, Sherwani MRK (2012) Laboratory observations on the larvicidal efficacy of three plant species against mosquito vectors of ma- laria, Dengue/Dengue Hemorrhagic Fe- ver (DF/DHF) and lymphatic filariasis in the semi-arid desert. J Environ Biol.33(3): 617–621.

Bilal H, Akram W, Ali-Hassan S (2012) Larvicidal Activity of Citrus Limonoids against Aedes albopictus Larvae. J Ar- thropod-Borne Dis. 6(2): 104–111.

Cavalcanti ESB, Maia de Moraisde S, Ashley ALM, Santana EWP (2004) Larvicidal activity of essential oils from Brazilian plants against Aedes aegypti L. Memó Insti Oswaldo Cruz. 99(5): 541–544.

David JP, Rey D, Pauntou MP, Meyran JC (2000) Differential toxicity of leaf lit- ter to dipteran larvae of mosquito de- velopmental sites. J Invertebr Pathol.75(1): 9–18.

Delahaye C, Rainford L, Nicholson A, Mitchell S, Lindo J, Ahmad M (2009) Antibac- terial and antifungal analysis of crude extracts from the leaves of Callistemon viminalis. J Med Bio Sci. 3(1): 190.

El-Gendy NA, Shaalan EA (2012) Oviposition deterrent activity of some volatile oils against the filarial mosquito vector Culex pipiens. J Entomol. 9: 435–441.

Finney DJ (1971) Probit analysis. III Edition.Cambridge University Press, London, pp. 20–42.

Islam M, Ali E, Saeed MA, Jamshaid M, Khan MTJ (2010) Antimicrobial and irritant activities of the extracts of Malva parviflora L., Malvastrum coromande- lianum L. and Amaranthus viridis L.-A preliminary investigation. Pak J Phar- macy. 20: 3-6.

Jiji T, Shonima GM, Pratheesh PT, Muraleed hara KG (2012) Larvicidal activity of indigenous plants against Culex quin- quefasciatus. J Environ Res and Dev.7(1A): 354–360.

Karmegan N, Sakthivadivel M, Anuradha V, Daniel T (1997) Indigenous plant ex- tracts as larvicidal agents against Culex quinquefasciatus Say. Biores Technol.5: 137–140.

Karmer WL, Mulla MS (1979) Oviposition attractant and repellents of mosquitoes: Oviposition response of Culex mos- quitoes to organic infusions. Environ Entomol. 8: 1111–1117.

Kaushik R, Saini P (2009) Screening of some semi-arid region plants for larvicidal activity against Aedes aegypti mosqui- toes. J Vector Borne Dis. 46: 244–246.

Khambay BPS, Beddie DG, Hooper AM, Simmonds MSJ, Green PWC (1999) New Insecticidal Tetradecahydroxanthenediones from Callistemon viminalis. J Nat Prod.62(12): 1666–1667.

Khare CP (2007) Indian Medicinal Plants: An Illustrated Dictionary. Springer- Verlag BerlinHeidelberg New York.

Kirtikar KR, Basu BD (2000) Indian Me- dicinal Plants. Vol 6. Sri Satguru Pub- lication, New Delhi, pp. 1828–1831.

Koul O, Walia S (2009) Comparing impacts of plant extracts and pure allelochemi- cals andimplications for pest control. CAB Reviews: Perspectives in Agri- culture, Veterinary Science, Nutrition and Natural Resources, 4, 049, Available from: http://www.cabi.org/cabreviews.

Nazar S, Ravikumar S, Williams GP, Ali MS, Suganthi P (2009) Screening of Indian coastal plant extracts for larvicidal ac- tivity of Culex quinquefasciatus. Ind J Sc and Technol. 3: 24–27.

Palsson K, Jaenson GT (1999) Plant products used as mosquito repellents in Guinea Bissau, West Africa. Acta Topica. 72 (1): 39–52.

Pialoux G, Gauzere BA, Jaurequiberry S, Strobel M (2007) Chikungunya, an epidemic arbovirosis. Lancet Infec Dis. 7: 319–327.

Prajapati V, Tripathi AK, Aggarwal KK, Khanuja SPS (2005) Insecticidal, repel- lent and oviposition deterrent activity of selected essential oils against Anoph- eles stephensi, Aedes aegypti and Culex quinquefasciatus. Bioresour Technol.96: 1749–1757.

Rajkumar S, Jebanesan A (2005) Oviposition deterrent and skin repellent activities of Solanum trilobatum leaf extract against the malarial vector Anopheles stephensi. J Insect Sc. 5: 15.

Rey D, Cuany A, Pautou MP, Meyran JC (1999) Differential sensitivity of mos- quito texa to vegetable tannins. J Chem Ecol. 25(3): 537–548.

Rezza G (2012) Aedes albopictus and the reemergence of Dengue. BMC Public Health, 12, 72, Available at: http://www. biomedcentral.com/1471-2458/12/72.

Ribeiro A, Santos LMST, Romanaha AJ, Veloso DP, Zani CL (1994) Flavonoids from Trixis vauthieri (Asteraceae) extract active in vitro against trypomastigote forms of Tripanosoma cruzi. Memó Inst Oswaldo Cruz. 89: 188.

Rutledge CR, Clark F, Curtis A, Sackett S (2003) Larval mosquito control. Tech- nical Bull Florida Mosq Cont Assoc.4: 16–19.

Senthilkumar N, Varma P, Gurusubramanian G (2009) Larvicidal and adulticidal ac- tivities of some medicinal plants against the malarial vector, Anopheles stephensi (Liston). Parasitol Res. 104: 237–244.

Sivagnaname N, Kalyanasundaram M (2004) Laboratory Evaluation of Methanolic Extract of Atlantia monophylla (Family: Rutaceae) against Immature Stages of Mosquitoes and Non-target Organisms. Memó Inst Oswaldo Cruz. 99(1): 115–118.

Sukumar K, Perich MJ, Boobar LR (1991)Botanical derivatives in mosquito con- trol: A review. J Am Mosq Cont Assoc.7: 210–237.

Sumba LA, Okoth K, Deng AL, Githure J, Knols BGJ, Beier JC, Hassanali A (2004) Daily oviposition patterns of the African malaria mosquito Anopheles gambiae Giles (Diptera: Culicidae) on different types of aqueous substrates. J Circadian Rhythms. 2: 6.

Tandon M, Shukla YN, Prapathi AK, Singh SC (1999) Insect antifeedant principles from Vernonia cinerea. Phytotherapy Res. 12: 195–199.

Tawatsin A, Asavadachanukorn P, Thavara U, Wongsinkongman P, Bansidhi J, Boonruad T, Chavalittumrong P, Soonthornchareonnon N, Komalamisra N, Mulla MS (2006) Repellency of es- sential oils extracted from plants in Thailand against four mosquito vectors (Diptera: Culicidae) and oviposition deterrent effects against Aedes aegypti (Diptera: Culicidae). Southeast Asian J Trop Med Public Health. 37(5): 915–931.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H (2011) Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia. 1(1): 98–106. Waliwitya R, Kennedy CJ, Lawenberger CA

(2009) Larvicidal and oviposition al- tering activity of monoterpenoids, trans- anethol and rosemary oil to the yellow fever mosquito Aedes aegypti (Diptera: Culicidae). Pest Manag Sci.65: 241–248.

WHO (2005) Guidelines for laboratory and field testing of mosquito larvicides, WHO/CDS/WHOPES/GCDPP/2005.13.

WHO (2011) Guidelines on the quality, safety and efficacy of dengue tetrava- lent vaccine (live attenuated) WHO/ DRAFT, (DEN) 1–93.

Xue R, Barnard DR, Ali A (2003) Laboratory evaluation of 18 repellent compounds as oviposition deterrents of Aedes albopictus and as larvicides of Aedes aegypti, Anopheles quadrimaculatus, and Culex quinquefasciatus. J Am Mosq Control Assoc. 19(4): 397–403.

How to Cite
Yadav R, Tyagi V, Tikar S, Sharma A, Mendki M, Jain A, Sukumaran D. Differential Larval Toxicity and Oviposition Altering Activity of Some Indigenous Plant Extracts against Dengue and Chikungunya Vector Aedes albopictus. J Arthropod Borne Dis. 8(2):174-185.
Original Article