Prevalence and Antibiotic Resistance of Gram-Negative Pathogenic Bacteria Species Isolated from Periplaneta americana and Blattella germanica in Varanasi, India
AbstractBackground: Cockroaches are among the medically important pests found within the human habitations that cause serious public health problems. They may harbor a number of pathogenic bacteria on the external surface with anti- biotic resistance. Hence, they are regarded as major microbial vectors. This study investigates the prevalence and antibiotic resistance of Gram-negative pathogenic bacteria species isolated from Periplaneta americana and Blattella germanica in Varanasi, India.Methods: Totally, 203 adult cockroaches were collected form 44 households and 52 food-handling establishments by trapping. Bacteriological examination of external surfaces of Pe. americana and Bl. germanica were carried out using standard method and antibiotics susceptibility profiles of the isolates were determined using Kirby-Bauer disc diffusion methods.Results: Among the places, we found that 54% had cockroache infestation in households and 77% in food- handling establishments. There was no significant different between the overall bacteria load of the external surface in Pe. americana (64.04%) and Bl. germanica (35.96%). However the predominant bacteria on cockroaches were Klebsiella pneumonia, Escherichia coli, Enterobacter aerogenes, and Pseudomonas aeruginosa. However, Kl. pneumoniae and Ps. aeruginosa were the most prevalent, drug-resistant strains were isolated from the cockroaches with 100% resistance to sulfamethoxazole/ trimethoprim and ampicillin. For individual strains of bacteria, Escherichia coli was found to have multi-resistance to four antibiotic tested, Citrobacter freundii four, Enterobacter aerogenes and Proteus mirabilis to three.Conclusion: Cockroaches are uniformly distributed in domestic environment, which can be a possible vector for transmission of drug-resistant bacteria and food-borne diseases.
Allotey J, Mpuchane S, Gashe BA, Simpanya M, Matsheka I (2009) Trapping of Blat- tella germanica (L) populations in human dwellings in Gaborone, Botswana. J Appl Zoo Res. 20(2): 175–188.
Anupurba S, Bhattacharjee A, Garg A, Sen MR (2006) Antimicrobial susceptibility of Pseudomonas aeruginosa isolated from wound infections. Indian J Der- matol. 51: 286–288.
Aqeel Ahmad, Anuradha Ghosh, Coby Schal, Ludek Zurek (2011) Insects in con- fined swine operations carry a large antibiotic resistant and potentially vir- ulent enterococcal community. BMC Microbiology. 11:23doi:10.1186/1471-2180-11-23.
Basu S, Ramchuran Panray T, Bali Singh T, Gulati AK, Shukla VK (2009) A pro- spective, descriptive study to identify the microbiological profile of chronic wounds in outpatients. Ostomy Wound Manage Jan. 55(1): 14–20.
Bradford PA (2001) Extended spectrum lac- tamases in the 21st century: characteri- zation, epidemiology, and detection of this important resistance threat. Clin Microbial Rev. 48: 933–951.
Burgess NRH, Chetwyn KN (1981) Associ- ation of cockroaches with an outbreak of dysentery. Trans R Soc Trop Med Hyg. 75: 332–333.
Census of India (2006) (Provisional). "Slum Population in Million Plus Cities (Mu- nicipal Corporations): Part A". Office of the Registrar General, India. 22 Janu- ary 2002. Retrieved 18 August 2006.
Census of India (2006) (Provisional). "Popu- lation, Population in the age group 0–6 and literates by sex– Urban Agglom- eration/Town: 2001". Office of the Reg- istrar General, India, pp. 53–54. Re- trieved 17 August 2006.
Census of India (2006) (Provisional). "Urban Agglomerations/Cities having popula- tion of more than one million in 2001". Office of the Registrar General, India.
July 2001. Retrieved 18 August 2006. Chaichanawongsaroj N, Vanichayatanarak K, Pipatkullachat T, Polrojpanya M, Som- kiatcharoen S (2004) Isolation of gramnegative bacteria from cockroaches trapped from urban environment. South- east Asian J Trop Med Public Health.35(3): 681–684.
Clinical Laboratory Standards (CCLS) (2010) Performance Standards for Antimicro- bial Disk Susceptibility Tests, Approved Standard, tenth ed. NCCLS,Villanova, PA.
Cloarec A, Rivault G, Fontaine F, LeGuyader A (1992) Cockroaches as carriers of bacteria in multifamily dwellings. Epi- demiology of Infection. 109.
Cochran DG (1982) Cockroaches-biology and control. WHO, Vector Biology and Con- trol Geneva, Switzerland, Series. 82.856.
Cotton MF, Wasserman E, Pieper CH, Theron DC, van Tubbergh D, Campbell G, Fang FC, Barnes AJ (2000) Invasive disease due to extended spectrum beta-lac- tamaseproducing Klebsiella pneumoniae in a neonatal unit: the possible role of cockroaches. J Hosp Infect. 44: 13–17.
Cruden DL, Markovetz AJ (1987) Microbial ecology of the cockroach gut. Ann Rev Microbial. 41: 617–643.
Czajka E, Pancer K, Kochman M, Gliniewicz A, Sawicka B, Rabczenko D, Stypulkowska MH, (2003) Charac- teristics of bacteria isolated from body surface of German cockroaches caught in hospitals. Przegl Epidemiol. 57:655–662.
Czech University of Agriculture, Prague, Czech Republic (Edited by Wm H Robinson, F Rettich and GW Rambo).
Emery CL, Weymouth LA (1997) Detection and clinical significance of extended spectrum β-lactamases in a tertiary- care medical center. J Clin Microbiol.35(8): 2061–2067.
Fotedar R, Banerjee U, Samantary JC, Shriniwas (1992) Vector potential of hospital houseflies with special refer- ence to Klebsiella species. Epidemiol Infect. 109: 143–147.
Fotedar R, Shriniwas UB, Banerjee U, Samantray JC, Nayar E, Verma A (1991a) Nosocomial infections: cock- roaches as possible vectors of drug-re- sistant Klebsiella. J Hosp Infect. 18:155–159.
Fotedar R, Shriniwas, Banerjee U, Saman- tray JC, Nayar E, Verma A (1991b) Nosocomial infections: cockroaches as possible vectors of drug-resistant Kleb- siella. J Hosp Infect. 18(2): 155–159.
Fotedar R, Bannerjee U (1992) Nosocomial fungal infections: study of the possible role of cockroaches (Blattella germanica) as vectors. Acta Trop. 50(40): 339–343.
Graczyk TK, Knight R, Gilman RH, Cran- field MR (2001) The role of non-biting flies in the epidemiology of human in- fectious diseases. Microb Infect. 3:231–235.
Graczyk TK, Knight R, Tamang L (2005) Me- chanical transmission of human proto- zoan parasites by insects. Clin Micro- biol Rev.18: 128–132.
Gratz NG (1999) Urbanization, arthropod and rodent pests and human health, In Proceedings of the 3rd International Conference on Urban Pests, pp. 51–58. Harwood RF, James MT (1979) Entomology in Human and Animal Health, seventh ed. Macmillan Publishing, New York, NY.
Health Protection Agency (2007) Identifica- tion of glucose non-fermenting Gram- negative rods National Standard Meth- od BSOP ID 17 Issue 2.
Health Protection Agency (2010) Identifica- tion of Enterobacteriaceae. National Standard Method BSOP ID 16 Issue 3.
Indian Meteorology Department (2010) Sea- sonal Weather Averages, Retrieved. Seasonal Weather Averages data from Indian Meteorology Department.
Kopanic RJ (1994) Cockroaches as vectors of Salmonella: laboratory and field tri- als. J Food Prot. 57: 125–132.
Le Guyader A, Rivault C, Chaperon J (1989) Microbial organisms carried by brown- banded cockroaches in relation to their spatial distribution in a hospital. Epi- demiol Infect. 102: 485–492.
Mackerras IM, Mackerras MJ (1949) An epi- demic of infantile gastroenteritis caused by Salmonella bovis morbificans. J Hyg.47: 166–181.
Marinésia Aparecida Prado, Milca Severino Pereira, Cleomenes Reis Ribeirão Preto (2006) Profile of Antimicrobial Resis- tance of Bacteria Isolated from Cock- roaches (Periplaneta americana) in a Brazilian Health Care Institution. Braz J Infect Dis. 10(1): 26–32.
Mpuchane S, Allotey J, Gashe BA, Matsheka MI, Coetzee A, Jordaan A, Oteng M (2005) Association between German cockroaches (Blattella germanica) and street food vending: Implications for food safety in Botswana, In Proceed- ings of the 5th International Confer- ence on Insect Pests in Urban Environ- ment, Suntech, Singapore (Edited by Lee Chang and WH Robinson), pp.123–130.
Navarro Risueño F, Miró Cardona E, Mirelis Otero B (2002) Interpretive reading of the antibiogram of enterobacteria. Enferm Infecc Microbiol Clin. 20:225–234.
Oothuman P, Jeffery J, Aziz AH, Abu Bakar E, Jegathesan M (1989) Bacterial patho- gens isolated from cockroaches trapped from paediatric wards in peninsular Malaysia. Trans R Soc Trop Med Hyg.83: 133–135.
Pai HH, Chen WC, Peng CF (2005) Isolation of bacteria with antibiotic resistance from household cockroaches (Pe. amer- icana and Blattella germanica). Acta Trop. 93: 259–265.
Pai HH, Ko YC, Chen ER (2003) Cock- roaches (Periplaneta americana and Blattella germanica) as potential mechanical disseminators of Entamoeba histolytica. Acta Trop. 87: 355–359.
Paterson DL, Hujer KM, Hujer AM, Yeiser B, Bonomo MD, Rice LB, Bonomo RA (2003) Extended-spectrum b-lactamases in Klebsiella pneumoniae bloodstream isolates from seven countries: domi- nance and widespread prevalence of SHV- and CTX-M-type b-lactamases. Antimicrob Agents Chemother. 47(11):3554–3560.
Paul S, Khan AM, Baqui MA, Muhibullah M (1992) Evaluation of the common cockroach Periplaneta americana (L) as carrier of medically important bac- teria. J Commun Dis. 24: 206–210.
Poole K (2004) Efflux-mediated multi- resistance in Gram-negative bacteria. Clin Microbiol Infect. 10(1): 12–26.
Prado MA, Pimenta FC, Hayashid M, Souza PR, Pereira MS, Gir E (2002) Entero- bacteria isolated from cockroaches (Pe- riplaneta americana) captured in a Bra- zilian hospital. Pan Am J Public Health.
Sudershan Rv, Pratima Rao, Kalpagam Polasa (2009) Food safety research in India: a review. As J Food ag-ind.2(03): 412–433.
Rivault G, Cloarec A, Le Guyader A (1993a) Bacterial load of cockroaches in rela- tion to urban environment. Epidemiol Infect. 110: 317–325.
Rivault G, Cloarec A, Le Guyader A (1993b) Bacterial contamination of food by cockroaches. J Environ Health. 55:21–22.
Rueger ME, Olson TA (1969) Cockroaches (Blattaria) as vectors of food poisoning and food infection organisms. J Med Entomol. 6: 185–189.
Saitou K, Furuhata K, Kawakami Y, Fuku- yama M (2009) Isolation of Pseudo- monas aeruginosa from cockroaches Captured in hospitals in Japan, and their antibiotic susceptibility. Biocontrol Sci Dec. 14(4): 155–159.
Status of landfill sites in 59 cities (2009) Management of municipal solid wastes. Planning Division, Central Pollution Control Board. Archived from the original on 17 July 2006. Retrieved 18 August 2009.
Stek M (1982) Cockroaches and enteric pathogens. Trans R Soc Trop Med Hyg. 76(4): 566–567.
Vythilingam I, Jeffery J, Oothuman P, Abdul Razak A, Sulaimman A (1997) Cock- roaches from urban human dwellings: Isolation of bacterial pathogens and control. Southeast Asian J Trop Med Public Health. 28: 218–222.
Waste Generation and Composition (2009) Management of municipal solid wastes. Planning Division, Central Pollution Control Board. Archived from the original on 17 July 2006. Retrieved 18 August 2009.
Bell WJ, Roth LM, Nalepa CA (2007) Cock- roaches: ecology, behaviour, and natu- ral history, Microbes: Unseen influence:76–88.
Williams KP, Gillespie JJ, Sobral BWS, Nordberg EK, Snyder EE, Shallom JM, Dickerman AW (2010) "Phylog- eny of Gammaproteobacteria". J Bac- teriol. 192(9): 2305–2314.
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