Baseline Susceptibility of Different Geographical Strains of Anopheles stephensi (Diptera: Culicidae) to Temephos in Malarious Areas of Irana.
AbstractBackground: Malaria still remains a public health problem in Iran. There are different vector control interventions such as insecticide spraying. The present study was carried out to determine the susceptibility status of Anopheles stephensi larvae to temephos as a national plan for monitoring and mapping of insecticide resistance Methods: Eight different localities in two main malarious provinces were determined as field collecting sites. Mos- quitoes were collected from the field and reared in an insectray. Susceptibility assays were carried out according to the WHO method. The laboratory reared susceptible Beech-Lab strain was used for comparison. Data were analyzed using Probit analysis to determine LC50 and LC90 values.Results: Susceptibility of An. stephensi to temephos indicated that the LC50 ranged from 0.0022 mg/l to 0.0141 mg/l. Although all field strains were susceptible to temephos, considerable variations in temephos resistance ratios of field strains were noticed from all the localities studied in comparison with the susceptible strain. A low level of resistance ratio was noticed in An. stephensi populations except for the Chabahar strain (RR= 4.27 fold). All field-collected An. stephensi populations exhibited homogeneity to the larvicide except for Bandar Abbas and Hormoodar village strains (P> 0.05%).Conclusion: Due to intensive use of temephos in the neighboring countries and occurrence of resistant to this insec- ticide in the main malaria vector in the region, insecticide resistance gene may evolve in the populations of An. stephensi. If temephos be applied as a larvicide it should be used judiciously for resistance management, as rotation strategy.
Abai MR, Mehravaran A, Vatandoost H, Oshaghi MA, Javadian E, Mashayekhi M, Mosleminia A, Piyazak N, Edallat H, Mohtarami F, Jabbari H, Rafi F (2008) Comparative performance of imagicides on Anopheles stephensi, main malaria vector in a malarious area, southern Iran. J Vector Borne Dis. 45(4): 307–312.
Abbott WS (1965) A method of comparing the effectiveness of an insecticide. J Econ Entomol. 18: 265–267.
Anderasen MH (2006) Emerging resistance to temephos in Anopheles stephensi in the Al-Dhahira Region of Oman. World Health Organization, Geneva. pp. 1–13.
Ansari MA, Mittal PK, Razdan RK, Dhiman RC, Kumar A (2004) Evaluation of pirimiphos-methyl (50% EC) against the immatures of Anopheles ste- phensi/An. culicifacies (malaria vec- tors) and Culex quinquefasciatus (vec- tor of bancroftian filariasis). J Vector Borne Dis. 41(1–2): 10–16.
Chavasse DC, Yap HH (1997) Chemical methods for the control of vectors and pests of public health importance. WHO/CTD/WHOPES/97.2, World Health Organization, Geneva.
Dash A P, Adak T, Raghavendra K, Singh OP (2007) The biology and control of malaria vectors in India. Current Sci.92: 1571–1578.
Davari B, Vatandoost H, Oshaghi MA, Ladonni H, Enayati AA, Shaeghi M, Basseri HR (2007) Selection of Anoph- eles stephensi with DDT and dieldrin and cross-resistance spectrum to py- rethroids and fipronil. Pestic Biochem Physiol. 89(2): 97–103.
Doosti S, Azari-Hamidian S, Vatandoost H,Oshaghi MA, Hosseini M (2006) Tax- onomic differentiation of Anopheles sacharovi and An.maculipennis s.l. (Diptera: Culicidae) by seta 2 (an- tepalmate hair) of larvae in Iran with a historical review and an annotated bib- liography of Iranian An. maculipennsi complex. Acta Med Iran. 44(1): 21–27.
Edrissian G (2006) Malaria past and present situation in Iran. Iran J Parasitol. 1(1):1–14.
Enayati AA, Vatandoost H, Ladonni H, Townson H, Hemingway J (2003) Mo- lecular evidence for a kdr-like py- rethroid resistance mechanism in the malaria vector mosquito Anopheles stephensi. Med Vet Entomol. 17(2):138–144.
Finney DJ (1971) Probit analysis, III edn. Cam- bridge University Press, Cambridge.
Hadjiakhoondi A, Vatandoost H, Khanavi M, Abaee MR (2005) Biochemical inves- tigation of different extracts and lar- vicidal activity of Tagetes minuta L on Anopheles stephensi larvae. Iran J Pharm Sci. 1: 81–84.
Hadjiakhoondi A, Sadeghipour-Roodsari HR, Vatandoost H, Khanavi M, Abaee MR, Vosoughi M (2006) Fatty acid composi- tion and toxicity of Melia azedarach L. fruits against malaria vector Anopheles stephensi. Iran J Pharm Sci. 2: 97–102.
Hanafi-Bojd AA, Vatandoost H, Jafari R (2006) Susceptibility status of Anoph- eles dthali and An. fluviatilis to com- monly used larvicides in an endemic focus of malaria, southern Iran. J Vec- tor Borne Dis. 43(1): 34–38.
Hanafi-Bojd AA, Vatandoost H, Oshaghi MA, Haghdoost AA, Shahi M, Sedaghat MM, Yeryan M, Pakari A (2012) En- tomological and epidemiological at- tributes for malaria transmission and implementation of vector control in southern Iran. Acta Trop. 121: 85–92.
Naddaf SR, Oshaghi MA, Vatandoost H, Asmar M (2003) Molecular character- ization of the Anopheles fluviatilis spe- cies complex in Iran. East Mediterr Health J. 9(3): 257–265.
Omer SM, Georghiou GP, Irving SN (1980) DDT/pyrethroid resistance interrela- tionships in Anopheles stephensi. Mosq News. 40: 200–209.
Omrani S-M, Vatandoost H, Oshaghi MA, Shokri F, Guerin PM, Ershadi MRY, Rassi Y, Tirgari S (2010) Fabrication of an olfactometer for mosquito behav- ioural studies. J Vector Borne Dis. 47 (1): 17–25.
Oshaghi MA, Sedaghat MM, Vatandoost H (2003a) Molecular characterization of the Anopheles maculipennis complex in the Islamic Republic of Iran. East Mediterr Health J. 9(4): 659–666.
Oshaghi MA, Yaghoobi F, Vatandoost H, Abai MR, Akbarzadeh K (2006) Anoph- eles stephensi biological forms, geo- graphical distribution, and malaria trans- mission in malarious regions in Iran. Pak J Biol Sci. 9(2): 294–298.
Ponlawat A, Scott JG, Harrington LC (2005) Insecticide Susceptibility of Aedes aegypti and Aedes albopictus across Thailand. J Med Entomol. 42(5): 821–825.
Raeisi A, Ranjbar M, Shoghli A, Vatandoost H, Faraji L (2008) National strategy plan for malaria control (IR Iran, 2004–2008).
Ministry of Health and Medical Education of Iran Publication, Tehran. Rafinejad J, Vatandoost H, Nikpoor F, Abai
MR, Shaeghi M, Duchen S, Rafi F (2008) Effect of washing on the bioefficacy of insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) against main malaria vector Anopheles stephensi by three bioassay methods. J Vector Borne Dis.45(2): 143–150.
Sadat Ebrahimi SE HA, Rezazadeh Sh, Fereidunian N, Vatandoost H, Abaei MR (2005) The components of Tagetes minuta L. and its biological activities against malaria vector, Anopheles ste- phensi in Iran. J Med Plants. 4: 43–47.
Sedaghat MM, Linton YM, Nicolescu G, Smith L, Koliopoulos G, Zounos AK, Oshaghi MA, Vatandoost H, Harbach RE (2003a) Morphological and molec- ular characterization of Anopheles (Anopheles) sacharovi Favre, aprimary vector of malaria in the Middle East. Syst Entomol. 28: 241–256.
Sedaghat MM, LintonYM, Oshaghi MA, Vatandoost H, Harbach RE (2003b) The Anopheles maculipennis complex (Diptera: Culicidae) in Iran: molecular characterizations and recognition of a new species. Bull Entomol Res. 93:527–535.
Sedaghat MM, Dehkordi AS, Khanavi M, Abai MR, Mohtarami F, Vatandoost H (2011) Chemical composition and lar- vicidal activity of essential oil of Cup- ressus arizonica EL Greene against ma- laria vector Anopheles stephensi Liston (Diptera: Culicidae). Pharmacognosy Res. 3(2): 135–139.
Shahi M, Hanafi-Bojd AA, Iranshahi M, Vatandoost H, Hanafi-Bojd AA (2010) Larvicidal efficacy of latex and extract of Calotropis procera (Gentianales: Asclepiadaceae) against Culex quin- quefasciatus and Anopheles stephensi (Diptera: Culicidae). J Vector Borne Dis. 47(3): 185–188.
Soltani A, Vatandoost H, Jabbari H, Mes- daghinia AR, Mahvi AH, Younesian M, Hanafi-Bojd AA, Bozorgzadeh S, Abai MR, Pakari A, Shabkhiz H (2008) Use of Expanded Polystyrene (EPS) and Shredded Waste Polysty- rene (SWAP) Beads for Control of Mosquitoes. Iran J Arthropod-Borne Dis. 2(2): 12–20.
Tikar SN, Mendki MJ, Chandel K, Parashar BD, Prakash S (2008) Susceptibility of immature stages of Aedes (Stegomyia) aegypti, vector of dengue and chi- kungunya to insecticides from India. Parasitol Res. 102(5): 907–913.
Tyagi BK, Yadav SP (2001) Bionomics of malaria vectors in two physiographically different areas of the epidemic-prone Thar Desert, north-western Rajasthan (India). J Arid Environ. 47(2): 161–172.
Vatandoost H (1996) The functional Basis of pyrethroids resistant in the malaria vector Anopheles stephensi [PhD dis- sertation]. University of Liverpool, UK.
Vatandoost H, Vaziri VM (2004) Larvicidal ac- tivity of a neem tree extract (Neemarin) against mosquito larvae in the Islamic Republic of Iran. East Mediterr Health J. 10(4–5): 573–581.
Vatandoost H, Shahi H, Abai MR, Hanafi-Bojd AA, Oshaghi MA, Zamani G (2004) Larval habitats of main malaria vectors in Hormozgan Province and their sus- ceptibility to different larvicides. South- east Asian J Trop Med Pub Hlth. 35 (2): 22–25.
Vatandoost H, Hanafi-Bojd AA (2005) Cur- rent Resistant Status of Anopheles stephensi Liston to Different Larvicides in Hormozgan Province, Southeastern Iran, 2004. Pak J Biol Sci. 8: 1568–1570.
Vatandoost H, Mashayekhi M, Abaie MR, Aflatoonian MR, Hanafi-Bojd AA, Sharifi I (2005) Monitoring of insecti- cides resistance in main malaria vec- tors in a malarious area of Kahnooj dis- trict, Kerman Province, southeastern Iran. J Vector Borne Dis. 42(3): 100–108.
Vatandoost H, Oshaghi M, Abai MR, Shahi M, Yaghoobi F, Baghai M, Hanafi- Bojd AA, Zamani G, Townson H (2006a) Bionomics of Anopheles ste- phensi Liston in the malarious area of Hormozgan Province, southern Iran. Acta Trop. 97(2): 196–205.
Vatandoost H, Shahi M, Hanafi-Bojd AA, Abai MR, Oshaghi MA, Rafii F (2007) Ecology of Anopheles dthali Patton in BandarAbbas. Iran J Arthropod-Borne Dis. 1(1): 21–27.
Vatandoost H, Hanafi-Bojd AA (2008) La- boratory evaluation of 3 repellents against Anopheles stephensi in the Islamic Republic of Iran. East Mediterr Health J. 14(2): 260–267.
Vatandoost H, Khazani A, Rafinejad J, Khoobdel M, Kebriai-Zadeh A, Abai MR (2008) Comparative efficacy of neem and dimethylphthalate (DMP) against malaria vector, Anopheles ste- phensi (Diptera: Culicidae). Asian Pac J Trop Med. 1(3): 1–6.
Vatandoost H, Abai MR, Abbasi M, Shaeghi M, Abtahi M, Rafie F (2009a) Design- ing of a laboratory model for evaluation of the residual effects of deltamethrin (K-othrine WP 5%) on different sur- faces against malaria vector, Anopheles stephensi (Diptera: Culicidae). J Vector Borne Dis. 46(4): 261–267.
Vatandoost H, Ramin E, Rassi Y, Abai MR (2009b) Stability and Wash Resistance of Local Made Mosquito Bednets and Detergents Treated with Pyrethroids against Susceptible Strain of Malaria Vector Anopheles stephensi. Iran J Arthropod-Borne Dis. 3(1): 19–28.
Vatandoost H, Akbarzadeh K, Hanafi-Bojd AA, Mashayekhi M, Saffari M, Elfatih MM (2010) Malaria stratification in a malarious area, a field exercise. Asian Pac J Trop Med. 3(10): 807–811.
WHO (1998) Test procedure for insecticide resistance monitoring in malaria vec- tors, bio-efficacy and persistence of in- secticides on treated surfaces, WHO/ CDS/CPC/MAL/98.12. World Health Organization, Geneva.
WHO (2012) Malaria Entomology and Vec- tor Control, Participant, s Guide. WHO, Geneva.
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