Wash Resistance and Bioefficacy of Alpha-cypermethrin Long Lasting Impregnated Nets (LLIN-Interceptor(®)) against Anopheles stephensi using Tunnel Test
Abstract
Background: The Long-lasing insecticide impregnated nets (LLINs) is considered as an effective tools for malaria vector control. The aim of this study was to evaluate the residual efficacy of alpha-cypermethrin long lasting im- pregnated nets (LLIN-Interceptor®) against Anopheles stephensi using tunnel test.
Methods: The wash-resistance of Interceptor® nets were assessed under laboratory conditions using tunnel test. Females of An. stephensi were released into the tunnel and then they were provided blood meals from guinea pigs.Bed nets were washed according to the standard procedure up to 20 times. The bioefficacy indicators such as inhi- bition of bloodmeal from experimental animal, knockdown, irritancy rate, survival rate, entry index and mortality were calculated.
Results: It induced 90–100% mortalities in the population of An. stephensi up to 15 washes. The KT50 values re- duced from 73.47 to 26.30 minutes in unwashed in comparison to one washed, respectively. The mean of mortality rate of blood-feeding inhibition and entry indexes was reached to 91.6%±2.8, 87.0±3.4 and 24.9±2.8 respectively after 20 washing.
Conclusion: This net could provide a good personal protection against malaria vectors and could induce relatively high mortality, inhibit the blood-feeding as well as reduce the entry rates of female mosquitoes even after several washes.
Abai MR, Mehravaran A, Vatandoost H, Oshaghi MA, Javadian E, Mashayekhi M, Mosleminia A, Piyazak N, Edallat H, Mohtarami F, Jabbari H, Rafi F (2008) Comparative performance of imagicides on Anopheles stephensi, main malaria vector in a malarious area, southern Iran. J Vector Borne Dis. 45(4): 307–312.
Abbot WS (1925) A method of computing the effectiveness of an insecticide. J Eco Entomol. 18: 265–267.
Banek K, Kilian A, Allan R (2010) Evaluation of Interceptor® long-lasting insecticidal nets in eight communities in Liberia. Malaria J. 9: 84.
Chandre F, Darriet F, Duchon S, Finot L, Manguin S, Carnevale P, Guillet P (2000) Modification of pyrethroid ef- fects associated with kdr mutation in Anopheles gambiae. Med Vet Entomol.14: 81–88.
Corbel V, Chandre F, Brengue C, Akogbéto M, Lardeux F, Hougard JM, Guillet P (2004) Dosage-dependent effects of permethrin-treated nets on the behavior of Anopheles gambiae and the selection of pyrethroid resistance. Malaria J.3(22): 1–9.
Davari B, Vatandoost H, Ladonni H, Shaeghi M, Oshaghi MA, Basseri HR, Enayati AA, Rassi Y, Abai MR, Hanafi-Bojd AA, Akbarzadeh K (2006) Comparative efficacy of different imagicides against different strains of Anopheles stephensi in the malarious areas of Iran, 2004–2005.
Pak J Biol Sci. 9: 885–892. Davari B, Vatandoost H, Oshaghi MA,Ladonni H, Enayati AA, Shaeghi M, Basseri HR, Rassi Y, Hanafi-Bojd AA (2007) Selection of Anopheles stephensi with DDT and dieldrin and cross- resistance spectrum to pyrethroids and fipronil. Pest Biochem Physiol. 89:97–103.
Doosti S, Azari-Hamidian S, Vatandoost H, Oshaghi MA, Hosseini M (2006) Taxonomic differentiation of Anopheles sacharovi and An. maculipennis S.l. Acta Med Iran. 44(1): 21–26.
Doosti S, Vatandoost H, Oshaghi MA, Hosseini M, Sedaghat MM (2007) Ap- plying morphometric variation of Seta 2 (Antepalmate Hair) among the larvae of the members of the Maculipennis Subgroup (Diptera: Culicidae) in Iran. Iran J Arthropod-Borne Dis. 1(1): 27–36.
Duchon S, Bonnet J, Corbel V (2005) Wash resistance and efficacy of long lasting insecticide nets from BASF against susceptible mosquitoes of Anopheles gambiae. Unpublished report to the WHO Pesticide Evaluation Scheme (WHOPES). In: Report of the Tenth WHOPES Working Group Meeting. WHO/CDS/NTD/WHOPES/2007.
Elissa N, Curtis CF (1995) Evaluation of dif- ferent formulations of deltamethrin in comparison with permethrin for impreg- nation of netting. Pest Sci. 44: 363–367.
Enayati AA, Vatandoost H, Ladonni H, Townson H, Hemingway J (2003) Mo- lecular evidence for a kdr-like pyrethroid resistance mechanism in the malaria vector mosquito Anopheles stephensi. Med Vet Entomol. 17: 138–144.
Finney DJ (1971) Probit Analysis. 3rd Ed.Cambridge University Press, Cambridge. Hanafi-Bojd AA, Azari-Hamidian S, Vatan- doost H, Zabihollah C (2011) Spatio- temporal distribution of malaria vectors (Diptera: Culicidae) across different climatic zones of Iran. Asian Pac J Trop Med. 4: 498–504.
Hougard JM, Duehon S, Darrirt F, Zaim M, Rogier C, Guillet P (2003) Comparative performances, under laboratory condi- tions, of seven pyrethroid insecticides used for impregnation of mosquito nets. Bull WHO. 81: 324–33.
Kayedi MH, Lines JD, Haghdoost AA, Najafi S (2007) A randomized and con- trolled comparison of the wash-re- sistances and insecticidal efficacies of four types of deltamethrin-treated nets, over a 6-month period of domestic use with washing every 2 weeks, in a rural area of Iran. Trop Med Parasitol. 101(6):519–528.
Kayedi MH, Lines JD, Haghdoost AA, Vatandoost H, Rassi Y, Khamisabadi K (2008) Evaluation of the effects of repeated hand washing, sunlight, smoke and dirt on the persistence of deltameth- rin on insecticide-treated nets. Trans R Soc Trop Med Hyg. 102(8): 811–816.
Kayedi MH, Lines JD, Haghdoost AA (2009) Evaluation of the wash resistance of three types of manufactured insecti- cidal nets in comparison to conven- tionally treated nets. Acta Trop. 111:192–196.
Gimnig JE, Lindblade KA, Mount DL, Atieli FK, Crawford, S, Wolkon A, Hawley WA, Dotson EM (2005) Labor- atory wash resistance of long-lasting insecticidal nets. Trop Med Inter Health.10: 1022–1029.
Gunasekaran K, Vaidyanathan K (2008) Wash resistance of PermaNets in comparison to hand-treated nets. Acta Trop. 105:154–157
Mosha FW, Lyino IN, Oxborough RM, Ma- towo J, Malima R, Feston E, Mndeme R, Tenu F, Kulkarni M, Maxwell CA, Magesa SM, Rowland MW (2008) Comparative efficacies of permethrin, deltamethrin and a-cypermethrin-treat- ed nets against Anopheles arabiensis and Culex quinquefasciatus in northern Tanzania. Ann Trop Med Parasitol.102(4): 367–376.
Omrani, SM, Vatandoost H, Oshaghi MA, Shokri F, Guerin PM, Yaghoobi- Ershadi MR, Rassi Y, Tirgari S (2010) Fabrication of an olfactometer for mos- quito behavioural studies. J Vector Borne Dis. 47: 17–25.
Oshaghi MA, Yaaghoobi F, Abai MR (2006a) Pattern of mitochondrial DNA variation between and within Anopheles stephensi (Diptera: Culicidae) biological forms suggests extensive gene flow. Acta Trop. 99: 226–233.
Oshaghi MA, Yaghoobi-Ershadi MR, Vatan- doost H, Abai MR, Akbarzadeh K (2006b) Anopheles stephensi biological forms, geographical distribution, and malaria transmission in malarious re- gions in Iran. Pak J Biol Sci. 9: 294–298.
Oxborough RM, Weir V, Irish S, Kaur H, N'Guessan R, Boko P, Odjo A, Metonnou C, Yates A, Akogbeto M, Rowland MW (2009) Is K-O Tab 1-2-3® long lasting on non-polyester mos- quito nets? Acta Trop. 112: 49–53.
Raeisi A, Shahbazi A, Ranjbar M, Shoghli A, Vatandoost H, Faraji L (2008) Na- tional strategy plan for malaria control in IR Iran, 2004–2008. Publication of Ministry of Health and Medical Ed- ucation. Tehran, Iran.
Rafinejad J,Vatandoost H, Nikpoor F, Abai MR, Shaeghi M, Duchen S, Rafi F (2008) Effect of washing on the bioefficacy of insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) against main malaria vector Anopheles stephensi by three bioassay methods. J Vector Borne Dis. 45:143–150.
Salari-Lak SH, Vatandoost H, Entezarmahd MR, Ashraf H, Abai MR, Nazari M (2002) Monitoring of insecticide re- sistance in Anopheles sacharovi (Favre1903) in borderline of Iran, Armenia, Naxcivan and Turkey, 2001. Iranian J Public Health. 31: 96–99.
Sedaghat MM, Linton YM, Nicolescu G, Smith L, Koliopoulos G, Zounos AK, Oshaghi, MA, Vatandoost H, Harbach RE (2003a) Morphological and molec- ular characterization of Anopheles (Anopheles) sacharovi Favre, a primary vector of malaria in the Middle East. Sys Entomol. 28: 241–256.
Sedaghat MM, Linton YM, Oshaghi MA, Vatandoost H, Harbach RE (2003b) The Anopheles maculipennis complex (Diptera: Culicidae) in Iran: molecular characterization and recognition of a new species. Bull Entomol Res. 93: 527–535.
Vatandoost H, Borhani N (2004a) Suscep- tibility level and irritability of synthet- ic pyrethroids against main malaria vectors in the endemic areas of Iran. Acta Med Iran. 42: 247–255.
Vatandoost H, Shahi H, Abai MR, Hanafi- Bojd AA, Oshaghi MA, Zamani G (2004b) Larval habitats of main ma- laria vectors in Hormozgan Province and their susceptibility to different larvicides. Southeast Asian J Trop Med Public Health. 35: 22–25.
Vatandoost H, Dehaki M, Djavadian E, Abai MR, Duchon S (2006a) Com- parative study on the efficacy of lambdacyhalothrin and bifenthrin on torn nets against the malaria vector, Anopheles stephensi as assessed by tunnel test method. J Vect Borne Dis.43(3): 133–135.
Vatandoost H, Gholizadeh MR, Abai MR, Djavadian E (2006b) Laboratory effi- cacy of protection rate of torn nets treat- ed with pyrethroids, cufluthrin, delta- methrin and permethrin against Anoph- eles stephensi (Diptera: Culicidae). J Biolo Sci. 6(2): 331–336.
Vatandoost H, Oshaghi MA, Abai, MR, Shahi M, Yaghoobi F, Baghaii M, Hanafi-Bojd AA, Zamani G, Townson H (2006c) Bionomics of Anopheles stephensi Liston in the malarious area of Hormozgan Province, southern Iran,2002. Acta Trop. 97: 196–203. Vatandoost H, Shamspour F, Abai MR
(2006d) Relative efficacy of different synthetic pyrethroids impregnated fab- rics (ITNs) against Anopheles stephensi in Iran. Pak J Biol Sci. 9(3): 503–506.
Vatandoost H, Hanafi-Bojd AA (2008) Laboratory evaluation of 3 repellents against Anopheles stephensi in the Islamic Republic of Iran. East Mediterr Health J. 2: 260–267.
Vatandoost H, Abai MR, Abbasi M, Shaeghi M, Abtahi M, Rafie F (2009a) Design- ing of a laboratory model for evalua- tion of the residual effects of delta- methrin (K-Othrine WP 5%) on different surfaces against malaria vector, Anoph- eles stephensi (Diptera: Culicidae). J Vector Borne Dis. 4: 261–267.
Vatandoost H, Ramin E, Rassi Y, Abai MR (2009b) Stability and wash resistance of local made mosquito bednets and detergents treated with pyrethroids against Anopheles stephensi. Iran J Arthropod-Borne Dis. 3(1): 19–28.
Vatandoost H, Zahirnia AH (2010) Respon- siveness of Anopheles maculipennis to different imagicides during resurgent malaria. Asian Pac J Trop Med. 3:360–363.
Vatandoost H, Emami SN, Oshaghi MA, Abai MR, Raeisi A, Piyazak N, Mahmoodi M, Akbarzadeh K (2011a) Ecology of malaria vector Anopheles culicifacies in a malarious area of Sistan va Baluchestan
Province, south-east Islamic Republic of Iran. East Mediterr Health J. 17:439–450.
Vatandoost H, Mamivandpoor H, Shayeghi M, Abai MR, Yaghoobi-Ershadi MR, Raeisi A, Abtahi M, Rafie F, Nikpour F (2011b) Evaluation of bioefficacy of α-cypermethrin in long lasting im- pregnated net (Interceptor®) using an- alytical method. Asian Pac J Trop Med.3(8): 642–646.
Vatandoost H, Rashidian A, Jafari M, Raeisi H, Hanafi-Bojd AA, Yousofzai AW, Daryanavard L, Mojahedi AR, Pakari A (2011c) Demonstration of malaria situation analysis, stratification and planning in Minab District, southern Iran. Asian Pac J Trop Med. 4: 67–71.
Yates A, N'Guessan R, Kaur H, Akogbeto M, Rowland M (2005) Evaluation of KO-Tab 1-2-3®: a wash-resistant 'dip-it- yourself insecticide formulation for long-lasting treatment of mosquito nets. Malaria J.
World Health Organization (2005) Guide- lines for laboratory and field testing of long-lasting insecticidal mosquito nets. WHO/CDS/WHOPES/GCDPP/2005.11.
World Health Organization (2007) WHO Global Malaria Programme: Position Statement on ITNs. WHO, Geneva.
World Health Organization (2012) Malaria Entomology and Vector Control. Par- ticipants' Guide. WHO. Switzerland, Geneva.
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Issue | Vol 7 No 1 (2013) | |
Section | Articles | |
Keywords | ||
Alpha-cypermethrin Anopheles stephensi LLINs Tunnel test |
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