Lectin Activity in Gut Extract of Culex pipiens
Abstract
Background: The role of lectins is important in interaction between pathogens and mosquito vectors. This study was performed to identify agglutinin activities of protein molecules on the midgut of Culex pipiens.
Methods: Culex pipiens was reared in insectray condition and the midguts of males and females (blood fed and un- fed) were dissected separately in Tris-HCl buffer. The extracts of midguts were applied for hemagglutinin assay against red blood cells of rabbit, mouse, rat, dog, horse, sheep, guinea pig, cow, human (A, B, AB, O groups). Then, the RBCs with relatively high agglutinin activity were chosen for carbohydrate inhibition assay. D (+) glucose, D (+) galactose, D (+) mannose, D (-) fructose, D (-) arabinose, L (-) fucose, lactose, N-acetyl-D-glucosamine, N-acetyl-Dgalactosamine, sialic acid were used to specify carbohydrate binding lectin.
Results: The highest agglutinin activities were found against sheep and rabbits RBCs. Sexual diversity of agglutinin activities was observed among midgut extraction of males and females. In addition, variation in agglutinin activity of blood fed and unfed female mosquitoes were detected. The lectin activity was inhibited highly with glucose, galactose, fucose and fructose but less inhibitor activities was observed by arabinose, N-acetyl-D-galactosamine, n- acetyl-d-glucosamine, lactose and mannose.
Conclusion: The secretion of hemagglutinins (lectins or lectin-like molecules) in the digestive system depends on the type of food in the gut. This suggests that emptying of the gut in preparation for protein rich food probably starts the secretion of hemagglutinins.
Ayaad TH (2009) Isolation of midgut agglu- tinin of Culex quinquefasciatus (Diptera: Culicidae). Egypt Acad J Biolog Sci.2(2): 55–68.
Azari-Hamidian (2007) Larval habitat char- acteristics of mosquitoes of the genus Culex (Diptera: Culicidae) in Guilan Province, Iran. Iran J Arthropod-Borne Dis. 1(1): 9–20.
Basseri HR (2002) Role of lectins in inter- action between parasites and the im- portant insect vectors. Iranian J Publ Health. 31: 69–74.
Basseri HR, Doosti S, Akbarzadeh K, Nateghpour M, Whitten MM, Ladoni H (2008) Competency of Anopheles stephensi mysorensis strain for Plas- modium vivax and the role of inhibitory carbohydrates to block its sporogonic cycle. Malar J. 15(7): 131.
Basseri HR, Safari N, Mousakazemi SH, Akbarzade K (2004) Comparison of midgut hemagglutination activity in three different geographical popula- tions of Anopheles stephensi. Iranian J Publ Health. 33(3): 60–67.
Billingsley PF, Sinden RE (1997) Determi- nants of malaria mosquito specificity. Parasitol Today. 13: 297–301.
Bradford MM (1976) A dye binding assay for protein. Anal Biochem. 72: 248–254.
Chugh M, Adak T, Sehrawat N, Gakhar SK (2011) Effect of anti-mosquito midgut antibodies on development of malaria parasite, Plasmodium vivax and fecundity in vector mosquito Anopheles culicifacies (Diptera: Culicidae). Indian J Exp Biol.49(4): 245–253.
Cox J, Brown HE, Rico-Hesse R (2011) Varia- tion in vector competence for dengue viruses does not depend on mosquito midgut binding affinity. PLoS Negl Trop Dis. 5(5): e1172.
Dehghan H, Sadraei J, Moosa-Kazemi SH (2010) The morphological variations of Culex pipiens Larvae (Diptera: Culicidae) in Yazd Province, Central Iran. Iran J Arthropod-Borne Dis. 4(2):42–49.
Gelbic I, Olejnícek J (2004) The haemagglutination activity in the dif- ferent developmental stages and changes of this activity caused by analogues of insect hormones in two members of the Culex pipiens complex of mosquitoes. Physiol Entomol. 29: 320–326.
Goldstein IJ, Poretz RD (1986) Isolation, phys- icochemical characterization and car- bohydrate-binding specificity of lectins. In: Liener IE, Sharon N and Goldstein IJ (Eds) The Lectins: Properties, Func- tions, and Applications in Biology and Medicine. Academic Press, San Diego, pp. 33–243.
Grubhoffer L, Hypsa V, Volf P (1997) Lectins (hemagglutinins) in the gut of the important disease vectors. Parasite. 4(3):203–216.
Grubhoffer L, Noreiga GF (1995) Midgut lectin of the mosquito Aedes aegypti. J Cell Biochem. 21A: 207.
Grubhoffer L, Musˇka M, Volf P (1994) Midgut haemagglutinins in five species of tsetse flies (Glossina spp): Two dif- ferent lectin systems in the midgut of Glossina tachinoides. Folia Parasitolo- gica. 41: 229–232.
Ibrahim E, Ingram G, Molyneux D (1984) Heamagglutinins and parasite agglutinins in hemolymph and gut of Glossina. Tropenmed Parasitol. 35(3): 151–156.
Ingram GA (1997) Detection and identifica- tion of parasite surface carbohydrates by lectins. In: Rogan MT (Eds) Analytic Parasitology. Springer, Berlin, pp. 304–319.
Ingram GA, Molyneux DH (1991) Insect lectins: Role in parasite–vector interac- tions. Lectin Rev. 1: 103–127.
Ingram GA, Molyneux DH (1988) Sugar spec- ificities antihuman ABO (H) blood group erythrocyte agglutinins (lectins) and he- molytic activity in the haemolymph and gut extract of three Glossina species. Insect Biochem. 18(3): 269–279.
Mello C, Nigam Y, Garcia E, Azambuja P, Newton R, Ratcliffe N (1999) Studies on a heamolymph lectin isolated from Rhodnius prolixus and its interaction with Trypanosoma rangeli. Exp Parasitol.91(4): 289–296.
Mohamed HA, Ingram GA, Molynux DH (1992) Carbohydrate binding specific- ities of anti erythrocyte lectins (hemagglutinins) in Anopheles gambiae gut extracts and hemolymph. Med Vet Entomol. 6: 217–224.
Mohamed HA, Ingram GA (1994) Effects of physiochemical treatments on hemagglutination activity of Anophe- les gambiae hemolymph and midgut extract. Med Vet Entomol. 8: 8–14.
Nayar JK, Knight JW (1997) Haemagglutinins in mosquitoes and their role in immune response to Brugia malayi (Filaroidea: Nematoda) larvae. Comp Biochem Physiol A. 118(4): 1321–1326.
Olejnicek J, Gelbic I, Grubhoffer L (2000) Changes in haemagglutination activity in the gut and abdomen of two strains of the Culex pipiens complex follow- ing glucose liquid sucking. Biologia, Bratislava. 55: 533–536.
Pereira MEA, Loures MA, Villalta F, An- drade AFB (1980) Lectin receptors as markers for Trypanosoma cruzi. J Exp Med. 152: 1375–1392.
Ratcliffe NA, Whitten MMA (2004) Vector- Immunity. In: Gillespie SH, Smith GL and Osborne A (Eds) SGM Symposium 63: Microbe-Vector Interactions in Vec- tor Borne Disease. Cambridge Universi- ty Press, Cambridge, pp. 199–262.
Rudin W, Hecker H (1989) Lectin-binding sites in the midgut of the mosquitoes Anopheles stephensi Liston and Aedes aegypti L. (Diptera: Culicidae). Parasitol Res. 75: 268–279.
Rudin W, Billingsley PF, Saladin S (1991) The fate of Plasmodium gallinaceum in Anopheles stephensi Liston and pos- sible barriers to transmission. Ann Soc Belg Med Trop. 71: 167–177.
Schottelius J (1982 a) Lectin binding strain specific carbohydrateson the cell sur- faces of Leishmania strains from the Old World. Z Parasitenkd. 66(3): 237–247.
Schottelius J (1982 b) Lectin typing of Try- panosoma vespertilionis and Try- panosoma cruzi. J Clin Chem Clin Biochem. 20: 131.
Schottelius J (1982c) Studies on the relation- ship between lectin binding carbohy- drates and different strains of Leish- mania from the NewWorld. Mem. Inst. Oswaldo Cruz, Rio Janeiro. 77: 19–27.
Stebbins MR, Hapner KD (1986) Isolation characterization and inhibition of arthropod agglutitins. In: Gupta AP (Ed) Hemocytic and Humoral Immunity in Arthropods. John Wiley and Sons, New York, pp. 463–491.
Tajedin L, Hashemi J, Abaei MR, Hosseinpour L, Rafei F, Basseri HR (2009) Study on fungal flora in the midgut of the larva and adult of the different popula- tions of the malaria vector Anopheles stephensi. Iran J Arthropod-Borne Dis.3(1): 36-40.
Uhlir J, Grubhoffer L, Volf P (1996) Novel ag- glutinin n the midut of the tick Ixodes ricinus. Folia Parasitol. 43: 233–239.
Volf P, Palanova L (1996) The relationship be- tween proteins content of the mealand lectin secretion in the midgut of Phle- botomus duboscqi. Ann Trop Med Pa- rasitol. 90(5): 567–70.
Volf P, Killick-Kendrick R (1996) Post-en- gorgement dynamics of haemaggluti- nation activity in the midgut of phle- botomine sandflies. Med Vet Entomol.10(3): 247–50.
Volf P (1993) Lectin activity in the gut extracts of sandfly Lutzomiya longipalpis. Folia Parasitol. 40: 155–156.
Volf P, Kiewegova´A, Svobodova M (1998) Sandfly midgut lectin: Effect of galac- tosamine on Leishmania major infec- tions. Med Vet Entomol. 12: 151–154.
Volf P, Killick-Kendrick R, Bates P, Molyneux DH (1994) Comparison of the hemagglutination activities in gut and head extracts of various species and geographical populations of phle- botomine sand flies. Ann Trop Med Parasitol. 88: 337–340.
Volf P, Pala´nova´ L, Svobodova´ M (1995) Midgut lectins of sandflies. Bull Direct Malariol and Environment Sanit. 35 (Suppl 1): 371–373.
Wallbanks KR, Ingram GA, Molyneux DH (1986) The agglutination of erythrocytes and Leishmania parasites by sand fly gut extracts: evidence for lectin activi- ty. Trop Med Parasitol. 37: 409–413.
Yoshida S, Shimada Y, Kondoh D, Kouzuma Y, Ghosh AK, Jacobs-Lorena M, Sinden RE (2007) Hemolytic C-type lectin CEL-III from sea cucumber expressed in transgenic mosquitoes impairs ma- laria parasite development. PLoS Pathog.3(12): e192.
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| Issue | Vol 7 No 1 (2013) | |
| Section | Articles | |
| Keywords | ||
| Culex pipiens Hemagglutination activity Lectin Midgut Mosquitoes | ||
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