Occult Dirofilariosis in Dogs of North Eastern Region in India

  • Sonjoy Kumar-Borthakur Mail Department of Parasitology, College of Veterinary Sciences and AH, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
  • Dilip Kumar-Deka Department of Parasitology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781 022, Assam, India.
  • Saidul Islam Department of Parasitology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781 022, Assam, India.
  • Prabhat Chandra-Sarmah Department of Parasitology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781 022, Assam, India.
Dirofilaria immitis, Occult infection, India


Background: The North Eastern Region in India is endemic for canine heartworm disease but in clinics accurate diagnosis is some times difficult. The aim of the present study was to determine the prevalence of occult infections for heartworm disease in canine in two geographical regions of North Eastern India.Methods: A total of 782 numbers of three categories of dogs namely, working dogs of military and paramilitary forces, pet dogs and stray dogs were screened for the presence of heartworm infection from August 2011 to July, 2012 in Guwahati (Assam) and Aizawl (Mizoram). Conventional, immunological and molecular techniques were followed for this epidemiological study. The criteria to determine the occult heartworm cases were based on the dif­ferences between heartworm positive cases in PCR test and antigen ELISA test.Results: The findings revealed an overall 22.69 percent occult case. The working dogs had highest prevalence (60%) followed by pet (29.16%) and stray dogs (17.75%).Conclusion: The highest percentage of occult heartworm infection was present in working dogs maintained under military or paramilitary forces. 


Alves LC, Silva LV, Faustino MAG, McCall JW, Supakonderj P, Labarthe NW, Sanchez M, Caires O (1999) Survey of Canine Heartworm in the city of Recife, Pernambuco, Brazil. Mem Inst Oswaldo Cruz, Rio de Janeiro. 94: 587–590.

Arellano PJL, Andrade MA, Lopez AJ, Carranaza C, Muro A (2002) Hel- minths and the Respiratory System. Arch. Bronconeumol. 42: 81–91.

Bemrick WJ, Sandholm HA (1966) Aedes vexans and other potential mosquito vectors of Dirofilaria immitis in Minne-sota. J Parasitol. 52: 762–767.

Bhattacharjee K (2011) Studies on haemo-parasitic infections of dog with special reference to Dirofilaria immitis. (PhD dissertation). Assam Agricultural Uni- versity, Khanapara, Guwahati-781 022, Assam, India.

Borthakur SK, Sarma K, Rajkhowa TK, Das MR, Rahman S (2006) Dirofilaria immitis infection in dog. J Vet Parasitol. 20: 167–169.

Borthakur SK, Ali MA, Patra G (2011) Clinical, haematological and biochem- ical studies on Dirofilaria immitis in dog. J Vet Parasitol. 25: 63–66.

Favia G, Lanfrancotti A, della Torre A, Cancrini G, Coluzzi M (1996) Poly- merase chain reaction- identification of Dirofilaria repens and Dirofilaria immitis. Parasitol. 113(6): 567–571.

Grieve RB, Glickman LT, Bater AK, Grieve MC, Thomas CB (1986) Canine Dirofilaria immitis infection in a hyperenzootic Area: Examination by parasitologic findings at necropsy and by two serorodiagnostic methods. Am J Vet Res. 7: 329–332.

Labarthe N, Almosny N, Guerrero J, Duque- Araújo, AM (1997) Description of the occurrence of canine Dirofilariasis in the State of Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz, Rio de Janeiro. 92: 47–51.

Lai CH, Ting CH, Tung KC, Wang JS (2001) Variation in the prevalence of dirofilariasis in stray dogs from Cen- tral Taiwan. J Chinese Soc Vet Sci. 27:69–73.

Lok JB (1988) Dirofilaria sp: taxonomy and distribution. In: Dirofilariasis. Boreham, PFL and Atwell RB (eds), CRC Press, Florida, pp. 1–28.

Ludlam KW, Jachowski LA, Otto GF (1970) Potential vectors of Dirofilaria immitis. J American Vet Med Assoc. 157:1354–1359.

Malmasi A, Hosseini SH, Aramoon M, Buhunar A, Seiff HA (2011) Survey of canine Dirofilaria immitis infection in Caspian provinces of Iran. Iran J Vet Res. 12: 340–343.

Megat Abd Rani PA, Irwin PJ, Gatne M, Coleman GT, Mclnnes LM (2010) A survey of canine filarial diseases of vet- erinary and public health significance in India. Parasites and Vectors. 3: 30.

Menezes R (2008) Rabies in India. Canadian Medical Assoc J. 178: 564.

Nadgir S, Tallur SS, Mangoli V, Halesh LH, Krishna BV (2001) Subconjunctival dirofilariasis in India. Southeast Asian J Trop Med Public Health. 32: 244–246.

Nath R, Gogoi R, Bordoloi N, Gogoi T (2010) Ocular dirofilariasis. Ind J Pathol Microbiol. 53: 157–159.

Nuchprayoon S, Junpee A, Poovorawan Y, Scoot AL (2005) Detection and dif- ferentiation of filarial parasites by uni- versal primer and polymerase chain reaction-restriction fragment length pol- ymorphism analysis. Am J Trop Med Hyg. 73(5): 895–900.

Rishniw M, Barr SC, Simpson KW, Frongillo MF, Franz M, Dominguez-Alpizar JL (2006) Description between six species of canine microfilariae by a single pol- ymerase chain reaction. Vet Parasitol.135: 303–314.

Sabu L, Devada K, Subramanian H (2005) Dirofilariosis in dogs and humans in Kerala. Ind J Med Res. 121: 691–693.

Senthivel K, Madhavan Pillai K (1999) A case of subcutaneous dirofilariosis in a woman in Kerala. Ind Vet J. 76: 263–264.

Shekar HS, Srinivasa H, Batru RR, Mathai E, Shariff S, Macaden RS (2000) Human ocular dirofilariasis in Kerala Southern India. Ind J Pathol Microbiol.43: 77–79.

Sudarshan MK, Mahendra BJ, Madhusudhana SN, Ashwoath Narayan DH, Rahman A, Rao NS, X-Meslin F, Lob D, Ravikumar KG (2006) An epidemi- ological study of animal bites in India: result of a WHO sponsored national multicentric rabies survey. J Commun Dis. 38(1): 32–39.

Yildiz K, Duru SY, Yagci BB, Ocal N, Gazyagci AN (2008) The prevalence of Dirofilaria immitis in dogs in Kirikkale. Türkiye Parazitoloji Dergisi.32: 225–228

How to Cite
Kumar-Borthakur S, Kumar-Deka D, Islam S, Chandra-Sarmah P. Occult Dirofilariosis in Dogs of North Eastern Region in India. J Arthropod Borne Dis. 10(1):92–97.
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