Short Communication

Species Composition and Ecological Aspects of Immature Mosquitoes (Diptera: Culicidae) in Bromeliads in Urban Parks in the City of São Paulo, Brazil


Background: Bromeliads can be epiphytic, terrestrial or saxicolous and use strategies to allow water to be re­tained in their leaf axils, where various arthropods can be found. These include mosquitoes, whose larvae are the most abundant and commonly found organisms in the leaf axils. The objective of this study was to look for im­mature forms of mosquitoes (the larval and pupal stages) in bromeliads in municipal parks in São Paulo and to discuss the ecological and epidemiological importance of these insects.Methods: From October 2010 to July 2013, immature mosquitoes were collected from bromeliads in 65 munici­pal parks in the city of São Paulo, Brazil, using suction samplers. The immature forms were maintained until adult forms emerged, and these were then identified morphologically.Results: Two thousand forty-two immature-stage specimens belonging to the genera Aedes, Culex, Trichoprosopon, Toxorhynchites, Limatus and Wyeomyia were found in bromeliads in 15 of the 65 parks visited. Aedes albopictus was the most abundant species (660 specimens collected), followed by Culex quinquefasciatus (548 specimens) and Cx. (Microculex) imitator (444). The taxa with the most widespread distribution were Ae. aegypti and Toxorhynchites spp, followed by Ae. albopictus and Cx. quinquefasciatus.Conclusion: Bromeliads in urban parks are refuges for populations of native species of Culicidae and breeding sites for exotic species that are generally of epidemiological interest. Hence, administrators and surveillance and mosquito-control agencies must constantly monitor these microenvironments as the presence of these species endangers the health of park users and employees as well as people living near the parks. 

Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI (2013) The global distribution and burden of dengue. Nature. 496(7446): 504–507.

Consoli RAG, Lourenço-de-Oliveira R (1994) Principais mosquitos de importância sanitária no Brasil. FIOCRUZ, Rio de Janeiro, p. 225.

Couto RDA, Latorre MDRDD, Di Santi, SM, Natal D (2010) Autochthonous malaria notified in the State of São Paulo: clinical and epidemiological characteristics from 1980 to 2007. Rev Soc Bras Med Trop. 43(1): 52–58.

Crovello TJ, Hacker CS (1972) Evolution- ary strategies in life table character- istics among feral and urban strains of Aedes aegypti (L). Evol. 26(2):185–196.

Derraik JGB, Heath ACG (2005) Immature Diptera (excluding Culicidae) inhab- iting phytotelmata in the Auckland and Wellington regions. New Zealand J Marine Freshw Res. 39(4): 981–987.

Dorvillé LFM (1996) Mosquitoes as bioindicators of forest degradation in Southeastern Brazil, a statistical eval- uation of published data in the litera- ture. Stud Neotrop Fauna Env. 31(2):68–78.

Duarte AMR, Pereira DM, de Paula MB, Fernandes A, Urbinatti PR, Ribeiro AF, Homem de Mello MHSH, Matos Jr MO, Mucci LF, Fernandes LN, Natal D, Malafronte RS (2013) Natu- ral infection in anopheline species and its implications for autochtho- nous malaria in the Atlantic forest in Brazil. Parasit Vectors. 6(1): 50–58.

Ferreira IB, Pereira LE, Rocco IM, Marti AT, de Souza LT, Iversson LB (1994) Surveillance of arbovirus in- fections in the Atlantic Forest Re- gion, State of São Paulo, Brazil. I. Detection of hemagglutination-inhib- iting antibodies in wild birds be- tween 1978 and 1990. Rev Inst Med Trop São Paulo. 36(3): 265–274.

Forattini OP (2002) Culicidologia Médica.Vol. 2. Edusp, São Paulo, p. 864. Frank JH, Sreenivasan S, Benshoff PJ,

Deyrup MA, Edwards GB, Halbert SE, Hamon AB, Lowman MD, Mockford EL, Scheffrahn RH, Steck GJ, Thomas MC, Walker TJ, Welbourn WC (2004) Invertebrate animals extracted from native Ti- llandsia (Bromeliales: Bromeliaceae) in Sarasota County, Florida. Florida Entomol. 87(2): 176–185.

Frank JH, Lounibos LP (2009) Insects and allies associated with bromeliads: a review. Terr Arthropod Rev. 1(2): 125–153.

Givnish TJ, Barffus MHJ, Van EB, Riina R, Schulte K, Horres R, Gonsiska PA, Jabaily RS, Crayn DM, Smith JAC, Inverno K, Brown GK, Evans TM, Holst BK, Luther H, Até W, Zizka G, Barry PE, Sytsma KJ (2011) Phy- logeny, adaptative radiation, and his- torical biogeography in Bromeliaceae: insights from an eight-locus plastid phylogeny. Am J Botany. 98(5): 872–895.

Harbach RE (2014) Mosquito Taxonomic Inventory. Available at: http:// mos-

IBGE (2014) Instituto Brasileiro de Geog- rafia e Estatística. Cidade de São Paulo. Available at: http://cidades.

Koh LP, Sodhil NS (2004) Importance of reserves, fragments, and parks for butterfly conservation in tropical ur- ban landscape. Ecol Appl. 14(6):1698–1708.

Lane J (1953) Neotropical Culicidae. Vol.2. Universidade de São Paulo, São Paulo, Brazil. pp. 565.

Lopes J (1997) Mosquito (Diptera: Culi- cidae) ecology in natural and arti- ficial rural breeding places in north- ern Parana, Brazil. V. Larvae collec- tion in artificial containers installed in ciliary Forest. Rev Saude Publ.31(4): 370–377.

Marques TC, Bourke BP, Laporta GZ, Sallum MAM (2012) Mosquito (Dip- tera: Culicidae) assemblages asso- ciated with Nidularium and Vriesea bromeliads in Serra do Mar, Atlantic Forest, Brazil. Parasit Vectors. 5(1):1–9.

McIntyre NE (2000) Ecology of urban ar- thropods: a review and a call to ac- tion. An Entomol Soc Am. 93(4):825–835.

McKinney ML (2008) Effects of urbaniza- tion on species richness: a review of plants and animals. Urban Ecosyst.11(2): 161–176.

Medeiros-Sousa AR, Ceretti Jr W, Urbinatti PR, Carvalho GC, Bicudo De Paula M, Fernandes A, Matos JR MO, Orico LD, Araujo AB, Nardi MS, Marrelli MT (2013) Mosquito fauna in Municipal parks of São

Paulo City, Brazil: A preliminary survey. J Am Mosq Control Assoc.29(3): 275–279.

Mocellin MG, Simões TC, Nascimento TFS, Teixeira MLFT, Lounibos LP, Oliveira RP (2009) Bromeliad-in- habiting mosquitoes in an urban bo- tanical garden of dengue endemic Rio de Janeiro. Are bromeliads pro- ductive habitats for the invasive vec- tors Aedes aegypti and Aedes albopictus? Mem Inst Oswaldo Cruz.104(8): 1171–1176.

Montes J (2005) Culicidae fauna of Serra da Cantareira, Sao Paulo, Brazil. Rev Saúde Pública. 39(4): 578–584.

Morais AS, Marrelli MT, Natal D (2006) Aspects of the distribution of Culex (Culex) quinquefasciatus Say (Dip- tera: Culicidae) in the region of the Pinheiros River, in the city of São Paulo, State of São Paulo, Brazil. Rev Bras Entomol. 50(3): 413–418.

Muller GA, Marcondes CB (2006) Brome- liad-associated mosquitoes from At- lantic forest in Santa Catarina Island, southern Brazil (Diptera, Culicidae), with new records for the State of Santa Catarina. Iheringia Sér Zool.96 (3): 315–319.

Murray KO, Mertens E, Desprès P (2010) West Nile virus and its emergence in the United States of America. Vet Res. 41(6): 67.

Natal D, Urbinatti PR, Taipe-Lagos CB, Ceretti-Jr W, Diederich ATB, Souza RG, Souza RP (1997) Aedes (Stego- myia) albopictus (Skuse) breeding in Bromeliaceae in the outskirts of an urban area of the city of São Paulo, Brazil. Rev Saude Publ. 31(5): 517–518.

Nyamah MA, Sulaiman S, Omar B (2011) Field observation on the efficacy of Toxorhynchites splendens (Wiede- mann) as a biocontrol agent against Aedes albopictus (Skuse) larvae in a cemetery. Trop Biomed. 28(2): 312–319.

Oliveira-Ferreira J, Lacerda MV, Brasil P, Ladislau JL, Tauil PL, Daniel- Ribeiro CT (2010) Malaria in Brazil: an overview. Mal J. 9: 115.

Pereira LE, Suzuki A, Coimbra TLM, Souza RD, Chamelet ELB (2001) Ilheus arbovirus in wild birds (Sporo- phila caerulescens and Molothrus bonariensis). Rev Saúde Pública. 35 (2): 119–123.

Reinert JF (2009) List of abbreviations for currently valid generic-level taxa in family Culicidae (Diptera). Eur Mos Bull. 27: 68–76.

Ribeiro AF, Urbinatti PR, de Castro Duarte AM, de Paula MB, Pereira DM, Mucci LF, Fernandes A, de Mello MH, de Matos Júnior MO, de Oliveira RC, Natal D, dos Santos Malafronte R (2012) Mosquitoes in degraded and preserved areas of the Atlantic Forest and potential for vec- tor-borne disease risk in the mu- nicipality of São Paulo, Brazil. J Vector Ecol. 37(2): 316–324.

São Paulo (2014a) Guia dos Parques Municipais de São Paulo. Prefeitura Municipal de São Paulo, SP, Brazil.4th ed. Secretaria do Verde e Meio Ambiente, São Paulo, p. 193.

São Paulo (2014b) Informe sobre Dengue.Secretaria de Saúde do Município de São Paulo, SP, Brazil. Available at: de/secretarias/upload/chamadas/infor me.

Souza RPD, Petrella S, Coimbra, TLM, Maeda AY, Rocco IM, Bisordi I, Silveira VR, Pereira LE, Suzuki A, Silva SJS, Silva FG, Salvador FS, Tubaki RM, Menezes RT, Pereira M, Bergo ES, Hoffmann RC, Spinola RMF, Tengan CH, Siciliano MM (2011) Isolation of yellow fever virus (YFV) from naturally infected Hae- magogus (Conopostegus) leucoce- laenus (Dipteral: Culicidae) in São Paulo State, Brazil, 2009. Rev Inst Med Trop São Paulo. 53(3): 133–139.

Taipe-Lagos CB, Natal D (2003) Abun- dância de culicídeos em área metro- politana preservada e suas implica- ções epidemiológicas. Rev Saúde Pública. 37(3): 275–279.

Urbinatti PR, Sendacz S, Natal D (2001) Imaturos de mosquitos (Diptera: Culi- cidae) em parque de área metropo- litana aberto à visitação pública. Rev Saúde Pública. 35(5): 261–266.

Viana DV, Ignotti EA (2013) Ocorrência da dengue e variações meteoroló- gicas no Brasil: revisão sistemática. Rev Bras Epidemiol. 16(2): 240–256.

WRBU (2014) Walter Reed Bio• systematics Unit. Systematic catalog of Culicidae. Smithsonian Institution, Washington DC, USA. Available at: http://www.

Weaver SC (2013) Urbanization and geo- graphic expansion of zoonotic arbo- viral diseases: mechanisms potential strategies for prevention. Trends Microbiol. 21(8): 360–363.

WHO (2013a) World Health Organization.Malaria entomology and vector con- trol: Guide for participants. Geneva, Switzerland.

WHO (2013b) World Health Organization.Lymphatic filariasis: managing mor- bidity and preventing disability: an aide-mémoire for national programme managers. Geneva, Switzerland.

Zequi AC, Lopes J, Medri IM (2005) Imaturos de Culicidae (Diptera) en- contrados em recipientes instalados em mata residual no município de Londrina, Paraná, Brasil. Rev Bras Zool. 22(3): 656–661.

IssueVol 10 No 1 (2016) QRcode
SectionShort Communication
Mosquitoes Bromeliads Municipal Parks Culicidae Breeding sites Immature Brazil

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Ceretti-Junior W, de-Oliveira-Christe R, Rizzo M, Claudia-Strobel R, Otavio-de-Matos-Junior M, Helena-Silva-Homem-de-Mello M, Fernandes A, Ralph-Medeiros-Sousa A, Cristina-de-Carvalho G, Toledo-Marrelli M. Species Composition and Ecological Aspects of Immature Mosquitoes (Diptera: Culicidae) in Bromeliads in Urban Parks in the City of São Paulo, Brazil. J Arthropod Borne Dis. 10(1):102–112.