Clinicopatho-Biochemical Alterations Associated with Subclinical Babesiosis in Dairy Animals
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Abstract
Background: Present investigation is based on the clinicopatho-biochemical alteration related to natural tick borne bovine babesiosis in Punjab state.
Methods: Blood samples from jugular vein of 542 bovines (cattle 466 and buffaloes 76) having history of tick infestation, fever, hemoglobinurea or anemia were collected and tested for Babesia bigemina by blood smear examination and PCR targeting 18S rRNA gene to distinguish clinically and subclinically infected groups. Further the hemato-biochemical parameters were correlated with the status of infection.
Results: Overall, of the 542 samples tested 16.42 % were positive by PCR while only 1.66 % by blood smear examination. The trend of molecular prevalence was found to decrease from north-eastern towards western Punjab. Analysis of the hematobiochemical alterations showed significant decrease in the levels of RBC, Hb, PCV, and MCV with significant increase in TBIL, MCH and MCHC levels.
Conclusion: As the transmission of B. bigemina is transovarian, presence of even few infected Rhipicephalus (Boophilus) microplus ticks on a subclinically infection can be the nidus of infection for whole herd, causing severe economic losses, at the same time significantly affecting the physiology of carrier animal.
Almeria S, Castella J, Ferrer D, Ortuno A, Estrada-Pena A, Gutierrez JF (2001) Bovine piroplasms in Minorca (Bale- aric Islands, Spain): A comparison of PCR-based and light microscopy de- tection. Vet Parasitol. 99: 249–259.
Altschul SF, Gish W, Miller W, Myers EW and Lipman DJ (1990) Basic local alignment search tool. J Mol Biol. 215:403–410.
Aulakh GS, Singla LD, Kaur P, Alka (2005) Bovine babesiosis due to Babesia bi- gemina: haematobiochemical and ther- apeutic studies. Ind J Anim Sci. 75:617–622.
Dwivedi SK, Sharma SP, Gautam OP (1976) Babesiosis: clinical cases in exotic and cross bred cattle. Ind Vet J. 55: 469–472.
Ellis J, Hefford C, Baverstock PR, Dalrym- ple BP, Johnson AM (1992) Riboso- mal DNA sequence comparison of Babesia and Theileria. Mol Biochem Parasitol. 54: 87–95.
Fahrimal Y, Goff WL, Jasmer DP (1992) Detection of Babesia bovis carrier cat- tle by using polymerase chain reaction amplification of parasite DNA. J Clin Microbiol. 30: 1374–1379.
Ferris DH, Todorovic R, Ristic M (1967) Infectitious Diseases of Man and Ani- mals. Vol 1. Academic Press, London.
Figueroa JV, Chieves LP, Johnson GS, Buening GM (1992) Detection of Babesia bigemina infected carriers by polymerase chain reaction amplifica- tion. J Clin Microbiol. 30: 2576– 2582.
Ghai JK, Singh M, Singh A (2008) Popula- tion dynamics of ixodid ticks infesting cattle in Bathinda and Hoshiarpur dis- tricts in the Punjab State. Annals Biol.24: 95–100.
Hussein AH, Mohammed N Abd-EL-Salam, Mohammed HK (2007) Theileriosis and babesiosis in cattle: haemogram and some biochemical parameters. Proceed- ings of XIII International Congress of ISAH, June17–21. Tartu, Estonia, pp.143–150.
Juyal PD, Kalra IS, Singla LD (1994) Prev- alence of haemoprotozoans in domes- tic animals in Punjab. Proceedings of the 6th national congress of veterinary parasitology, 22–24 Oct 1994, Jawahar- lal Nehru Krishi Vishwavidyalaya, Jab- alpur.
Kahn CM, Line S, Aiello SE (Eds) (2005) Reference Guides Table 7: Serum bio- chemical Reference Ranges. In: Merck Veterinary Manual 9th Edn, Merck and Co, Whitehousestation, USA, p. 89.
Latimer KS, Mahaffey EA, Prasse KW (2005) Serum biochemical reference ranges. In: Kahn CM, Line S, Aiello SE (Eds.): Merck Veterinary Manual,9th Ed, Merck and Co, White house station, USA, p. 2586.
McLeod R, Kristjanson P (1999) Tick cost: economic impact of ticks and TBD to livestock in Africa, Asia and Australia. International Livestock Research In- stitute (ILRI), Nairobi, Kenya.
Pandey N, Misra S (1987) Haematological and Biochemical response to haemo- lyticanaemia of clinical Babesiosis in cattle and therapy. Ind Vet J. 64: 882–886.
Passos LM, Bell-Sakyi L, Brown CG (1998) mmunochemical characterization of in vitro culture-derived antigens of Babesia bovis and Babesia bigemina. Vet Parasitol. 76: 239–249.
Ruprah MS (1985) A Text Book of Clinical Protozoology. Oxonian Press Pvt. Ltd, New Delhi.
Sharma AK, Katoch RC, Nagal KB, Kisht- waria RS, Sharma SK (2000) Bovine Babesiosis in Palam valley of Hima- chal Paradesh. Ind Vet J. 77: 731–732.
Singh AP, Singla LD Singh A (2000) A study on the effects of macroclimatic factors on the seasonal population dy- namics of Boophilus micropus (Canes 1888) infesting the cross-bred cattle of Ludhiana district. Int J Anim Sci. 15 (1): 29–31.
Singh J, Tuli A and Singla LD (2003) Mixed Anaplasma and Babesia infection out- break in crossbred cattle. Punjab Vet J.3(2): 73–74
Thrusfield M (2005) Veterinary Epidemiol- ogy, second edition. Blackwell Science Ltd, UK.
Vohara SP, Patel JV, Patel BB, Patel SB, Umale RH (2012) Seasonal incidence of haemoprotozoan diseases in crosses bred cattle and buffaloes in Kaira and Anand districts of Gujarat, India. Vet World. 5: 223–224.
Yeruham I, Avidar Y Aroch I, Hadani A (2003) Intra-uterine infection with Babesia bovis in a 2-day- old calf. J Vet Med B Infect Dis Vet Public Health. 50: 60–62.
| Files | ||
| Issue | Vol 10 No 2 (2016) | |
| Section | Short Communication | |
| Keywords | ||
| Babesiosis Dairy animals Haemato-biochemical Prevalence PCR | ||
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