Original Article

High Insecticides Resistance in Culex pipiens (Diptera: Culicidae) from Tehran, Capital of Iran


Background: During recent years transmission of Dirofilaria immitis (dog heart worm) by Culex pipiens and West Nile virus have been reported from Iran. The present study was preformed for evaluating the susceptibility status of Cx. pipiens collected from capital city of Tehran, Iran.
Four Insecticides including: DDT 4%, Lambdacyhalothrin 0.05%, Deltamethrin 0.05% and Cyfluthrin 0.15 % according to WHO standard  methods were used for evaluating the susceptibility status of Cx. pipiens from Tehran moreover  For comparison susceptibility status a Laboratory strain also was used.  Bioassay data were ana­lyzed using Probit program. The lethal time for 50% and 90% mortality (LT50 and LT90) values were calculated from regression line.
The susceptibility status of lab strain of Cx. pipiens revealed that it is susceptible to Lambdacyhalothrin, Deltamethrin, Cyfluthrin and resistant to DDT. Moreover cyfluthrin with LT50=36 seconds and DDT with LT50=3005 seconds had the least and most LT50s. Field population was resistance to all tested insecticides and DDT yielded no mortality.
Highly resistance level against all WHO recommended imagicides were detected in field populations. We suggest more biochemical and molecular investigations to detect resistance mechanisms in the field population for further decision of vector control.

Abai MR, Mehravaran A, Vatandoost H, Oshaghi MA, Javadian E, Mashayekhi M, Mosleminia A, Piyazak N, Edallat H, Mohtarami F, Jabbari H, Rafi F (2008) Comparative performance of imagicides on Anopheles stephensi, main malaria vector in a malarious area, southern Iran. J Vector Borne Dis. 45(4): 307–312.

Abbott WS (1965) A method of comparing the effectiveness of an insecticide. J Econ Entomol. 18: 265–267.

Ataie A, Moosa-Kazemi SH, Vatandoost H, Yaghoobi-Ershadi MR, Bakhshi H, Anjomruz M (2015) Assessing the Susceptibility Status of Mosquitoes (Diptera: Culicidae) in a Dirofilariasis Focus, Northwestern Iran. J Arthro- pod-Borne Dis. 9(1): 7–21.

Azari-Hamidian S, Yaghoobi-Ershadi MR, Javadian E, Abai MR, Mobedi I, Lin- ton YM, Harbach RE (2009) Distribution and ecology of mosquitoes in a focus of dirofilariasis in northwestern Iran, with the first finding of filarial larvae in naturally infected local mosquitoes. Med Vet Entomol. 23(2): 111–121.

Azari-Hamidian S, Yaghoobi-Ershadi MR, Javadian E, Mobedi I, Abai MR (2007) Review of dirofilariasis in Iran. J Med Fac Guilan Univ Med Sci. 15(60):102–113 (In Persian).

Ben Cheikh H, Ben Ali-Haouas Z, Marquine M, Pasteur N (1998) Resistance to organophosphorus and pyrethroid in- secticides in Cx. pipiens (Diptera: Culi- cidae) from Tunisia. J Med Entomol.35: 251–260.

Bisset JA, Rodriguez MM, Diaz C, Alain Soca L (1999) Characterization of re- sistance to organophosphate insecti- cides, carbamates, and pyrethroids in Culex quinquefasciaus from the State of Miranda, Venzuela. Rev Cubana Med Trop. 51(2): 89–94.

Bisset J, Rodríguez M, Díaz C, Ortiz E, Marquetti M, Hemingway J (1990) The mechanisms of organophosphate and carbamate resistance in Culex quinque- fasciatus (Diptera: Culicidae) from Cuba. Bull Entomol Res. 80(3): 245–250.

Calhoun LM, Avery M, Jones L, Gunarto K, King R, Roberts J, Burkot TR (2007) Combined sewage overflows (CSO) are major urban breeding sites for Culex quinquefasciatus in Atlanta, Geor- gia. Am J Trop Med. 77: 478–484.

Chen L, Zhong D, Zhang D, Shi L, Zhou G, Gong M, Zhou H, Sun Y, Ma L, He J, Hong S, Zhou D, Xiong C, Chen C, Zou P, Zhu C, Yan G (2010) Molecu- lar ecology of pyrethroid knockdown resistance in Culex pipiens pallens mosquitoes. PloS one. 5(7): e11681.

Corbel V, N'Guessan R, Brengues C, Chandre F, Djogbenou L, Martin T, Akogbéto M, Hougard JM, Rowland M (2007) Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa.Acta Trop. 101: 207–216.

Davidson G (1964) DDT resistance and dieldrin resistance in Cx. pipiens fatigans. Ann Trop Med Parasitol. 58:180–188.

Enayati AA, Vatandoost H, Ladonni H, Townson H, Hemingway J (2003) Mo- lecular evidence for a kdr-like pyrethroid resistance mechanism in the malaria vector mosquito Anopheles stephensi. Med Vet Entomol. 17(2):138–144.

Fathian M, Vatandoost H, Moosa-Kazemi SH, Raeisi A (2015) Susceptibility of Culicidae Mosquitoes to Some Insecti- cides Recommended by WHO in a Ma- laria Endemic Area of Southeastern Iran. J Arthropod-Borne Dis. 9(1): 22–34..

Finney DJ (1971) Probit analysis, III edn.Cambridge University Press, Cambridge. Golestani J (1967) The methods of the mos- quito Culex control in Tehran City. J General Med Tehran Univ Med School.6: 376–379. (In Persian).

Hanafi-Bojd AA, Vatandoost H, Jafari R (2006) Susceptibility status of Anoph- eles dthali and An. fluviatilis to com- monly used larvicides in an endemic focus of malaria, southern Iran. J Vector Borne Dis. 43(1): 34–38.

Hanafi-Bojd AA, Vatandoost H, Oshaghi MA, Haghdoost AA, Shahi M, Sedaghat MM, Yeryan M, Pakari A (2012) En- tomological and epidemiological attrib- utes for malaria transmission and im- plementation of vector control in south- ern Iran. Acta Trop. 121: 85–92.

Hanafi-Bojd AA, Vatandoost H, Philip E, Stepanova E, AI Abdi A, Safari R, Mohseni GH, Bruhi MI, Peter A, Ab- dulrazag SH, Mangal G (2010) Ma- laria situationanalysis and stratification in Bandar Abbas County, Southern Iran,2004– 2008. Iran J Arthropod-Borne Dis. 4: 31–41.

Horsfall WR (1955) Mosquitoes: Their Bio- nomics and Relation to Disease. Hafner publishing, New York, pp.723.

Jones CM, Machin C, Mohammed K, Majambere S, Ali AS, Khatib BO, Kelly-Hope LA (2012) Insecticide re- sistance in Culex quinquefasciatus from Zanzibar: implications for vector con- trol programmes. Parasit Vectors. 5: 78.

Lines J D (1988) Do agricultural insecticides select for insecticide resistance in mos-quitoes: A look at the evidence Par- asitology Today. 4: 17–20.

Lotfi MD (1976) Key to Culicinae of Iran, genus Culex and their biology (Diptera: Culicidae). Iranian J Publ Health. 5:71–84.

Lotfi MD., Manouchehri AV, Yazdanpanah H (1975) Resistance of Cx. pipiens pipiens to DDT in Northern Iran,1973. Bull Sot Pathol Exotique et de sefiliales. 68(1): 91.

Martinez-Torres D, Chevillon C, Brun-Barale A, Berge JB, Pasteur N, Pauron D (1999) Voltage-dependent Na+ channels in pyrethroid-resistant Cx. pipiens L mosquitoes. Pestic Sci. 55: 1012–1020.

Mitchell CJ, Francy DB, Monath TP (1980) Arthropod vectors. In: Monath TP, Reeves WC, editors. St. Louis Enceph- alitis. Washington: American Public Health Association. 313–379.

Mukhopadhyay AK, Sinha SN, Yadav RL, Narasimham MV (1993) Susceptibility status of Culex quinquefasciatus in Patna to insecticides. Indian J Publ Health.37(2): 57–60.

Mullen GR, Durden L (2009) Medical and Veterinary Entomology, Mosquitoes (Culicidae) Woodbridge A. Foster and Edward D Walter. Vol. 2. Elsevier, Burlington, pp. 207–260.

Naficy K, Saidi S (1970) Serological survey on viral antibodies in Iran. Trop Geogr Med. 2: 183–188.

Nazari M, Janbakhsh B (2000) a survey of the susceptibility level of Culex theileri and Cx. pipiens to DDT, Dieldrin, Propoxur and Malathion in the south- ern area of Tehran. J Uromia Univ Med Sci. 11(1): 13–19.

Nazni WA, Lee, HL, Azahari AH (2005) Adult and larval insecticide suscepti- bility status of Culex quinquefasciatus (Say) mosquitoes in Kuala Lumpur, Malaysia. Trop Biomed. 22: 63–68.

Oshaghi MA, Vatandoost H, Gorouhi A, Abai MR, Madjidpour A, Arshi S, Mehravaran A (2011) Anopheline species composi- tion in borderline of Iran, Azerbai- jan. Acta trop. 119(1): 44–49.

Pocquet N, Milesi P, Makoundou P, Unal S, Zumbo B, Atyame C, Darriet F, Dehecq J, Thiria J, Bheecarry A, Iyaloo D, Weill M, Chandre F, Labbé P (2013) Multiple Insecticide Resistances in the Disease Vector Culex quinquefasciatus from Western Indian Ocean. PLoS One. 8(10): e77855.

Sarkar M, Bhattacharyya I, Borkotoki A, Goswami D, Rabha B, Baruah I, Srivastava RB (2009) Insecticide re- sistance and detoxifying enzyme ac- tivity in the principal bancroftian filariasis vector, Culex quinquefasciatus, in northeastern India. Med vet entomol.23(2): 122–131.

Sathantriphop S, Paeporn P, Supaphathom K (2006) Detection of insecticides re- sistance status in Culex quinquefasciatus and Aedes aegypti to four major groups of insecticides. Trop Biomed. 23: 97–101.

Savage HM, Aggarwal D, Apperson CS, Katholi CR, Gordon E, Hassan HK, Unnasch TR (2007) Host choice and West Nile virus infection rates in blood- fed mosquitoes, including members of the Cx. pipiens complex, from Mem- phis and Shelby County, Tennessee,2002–2003. Vector-Borne Zoonot Dis.7: 365–386.

Service MW (2003) Medical Entomology for Students. Vol. 3. United Kingdom: Cambridge. University Press, ambridge.

Shahi M, Vatandoost H, Abaei MR, Hanafi- Bojd AA (2006) Susceptibility of Anoph- eles fluviatilis James to different insec- ticides in Bandar Abbas township, 2003. Hormozgan Med J. 10(4): 321–328.

Smith JL, Fonseca DM (2004) Rapid assays for identification of members of the Culex (Culex) pipiens complex, their hybrids, and other sibling species (Diptera: Culicidae). Am J Trop Med Hyg. 70: 339–345.

Soltani A, Vatandoost H, Oshaghi MA, Enayati AA, Raeisi A, Eshraghian, MR,Rafi F (2013) Baseline Suscepti- bility of Different Geographical Strains of Anopheles stephensi (Diptera: Culi- cidae) to Temephos in Malarious Areas of Irana J Arthropod-Borne Dis. 7(1):56–65.

Strickman D,Fonseca DM (2012) Autogeny in Cx. pipiens complex mosquitoes from the San Francisco bay area. Am J Trop Med Hyg. 87(4): 719–726.

Tantely ML, Tortosa P, Alout H, Berticat C, Berthomieu A, Rutee A, Weill M (2010) Insecticide resistance in Cx. pipiens quinquefasciatus and Aedes albopictus mosquitoes from La Reunion Island. Insect Biochem Mol Biol. 40: 317–324.

Toma L, Menegon M, Romi R, De Matthaeis E, Montanari M, Severini C (2011) Status of insecticide resistance in Culex pipiens field populations from north- eastern areas of Italy before the with- drawal of OP compounds. Pest Manag Sci. 67(1): 100–106.

Vatandoost H, Borhani N (2004) Suscepti- bility level and irritability of synthetic pyrethroids against main malaria vec- tors in the endemic areas of Iran. Acta Med Iran. 42: 247–255.

Vatandoost H, Hanafi-Bojd AA (2005) Cur- rent Resistant Status of Anopheles stephensi Liston to Different Larvicides in Hormozgan Province, Southeastern Iran, 2004. Pak J Biol Sci. 8: 1568–1570.

Vatandoost H, Mashayekhi M, Abaie MR, Aflatoonian MR, Hanafi-Bojd AA, Sharifi I (2005) Monitoring of insecti- cides resistance in main malaria vec- tors in a malarious area of Kahnooj district, Kerman Province, southeast- ern Iran. J Vector Borne Dis. 42(3):100–108.

Vatandoost H, Ezeddinloo L, Mahvi AH, Abai MR,Kia EB, Mobedi I (2004) Enhanced tolerance of house mosquito to different insecticides due to agri- cultural and household pesticides in sewage system of Tehran, Iran. Iranian J Environ Health Sci Eng. 1(1): 42–45.

Vinogradova EB (2000) Cx. pipiens pipiens Mosquitoes: Taxonomy, Distribution Ecology, Physiology, Genetics and Con-trol. Pensoft Publisher, Sofia.

WHO (1981) Instruction for determining the susceptibility or resistance of adult mosquitoes to organochlorine, organ- phosphate and carbamate insecticides. Diagnostic test. WHO/VBC.81.806.

WHO (2013) Test procedures for insecticide resistance monitoring in malaria vector mosquitoes. World Health Organization, Geneva, Switzerland.

IssueVol 10 No 4 (2016) QRcode
SectionOriginal Article
Susceptibility status Culex pipiens Tehran

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How to Cite
Salim-Abadi Y, Oshaghi MA, Enayati AA, Abai MR, Vatandoost H, Eshraghian MR, Mirhendi H, Hanafi-Bojd AA, Gorouhi MA, Rafi F. High Insecticides Resistance in Culex pipiens (Diptera: Culicidae) from Tehran, Capital of Iran. J Arthropod Borne Dis. 2016;10(4):483-492.