Evaluation of Oxalic Acid treatments against the Mite Varroa destructor and Secondary Effects on Honey Bees Apis mellifera
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Abstract
Background: The Varroa destructor varroasis is a very serious parasite of honeybee Apis mellifera. The objective of this study was to evaluate the effectiveness of Varroa treatment using organic acid (oxalic acid) in Algeria identifying its side effects on bee colonies.
Methods: Treatment was conducted in one apiary consisting 30 colonies kept in Langstroth hives kind. Oxalic acid dripped directly on bees 5ml of this solution of oxalic acid per lane occupied by a syringe. Three doses were tested: 4.2, 3.2 and 2.1% oxalic acid is 100, 75 and 50 g of oxalic acid dehydrate in one litter of sugar syrup (1water to1 surge) concentration. Results: The percentage of average efficiency obtained for the first dose was 81%, 72.19% for the second dose, and 65% for third one, while the dose of 100 g oxalic acid causes a weakening of honey bee colonies.
Conclusion: The experiments revealed that clear variation in the treatment efficiency among colonies that this might be related to brood presence therefore in order to assure the treatment efficiency oxalic acid should be part of a bigger strategy of Varroa treatment.
Adjlane N, Doumandji SE, Haddad N (2012) Situation de l'apiculture en Algérie: fac- teurs menaçant la survie des colonies d'abeilles locales Apis mellifera inter- missa. Cah Agric. 21: 235–41.
Adjlane N, Doumandji SE, Haddad N (2013)
Varroa destructor resistance to fluvali- nate in Algeria. Trends Entomol. 12:123–125
Anderson DL, Trueman JWH (2000) Varroa jacobsoni (Acari: Varroidae) is more than one species. Exp Appl Acarol. 24:165–189.
Bacandritsos N, Papanastasiou I, Saitanis C, Nanetti A, Roinioti E (2007) Efficacy of repeated trickle applications of ox- alic acid in syrup for varroosis control in Apis mellifera: Influence of meteorolog- ical conditions and presence of brood. Vet Parasitol. 148: 174–178.
Boecking O, Genersch E (2008) Varroosis- the Ongoing Crisis in BeeKeeping. J Verbr Lebensm. 2: 221–228.
Bowen-Walker PL, Martin SJ, Gunn A (1999) The transmission of Deformed Wing Virus between honeybees (Apis mellif- era L.) by the ectoparasitic mite Var- roa jacobsoni Oud. J Invertebr Pathol.73: 101–106.
Bowen-Walker PL, Gunn A (2001) The effect of the ectoparasitic mite, Varroa de- structor on adult worker honeybee (Apis mellifera) emergence weights, water, protein, carbohydrate, and lipid levels. Entomol Exp Appl. 101(3): 101–112.
Büchler R (1998) Überprüfung der Bienen- verträglichkeit von Oxalsäure an Kun- stschwärmen, Allg. Dtsch Imkerztg.11: 21–23.
Charrière JD (2001) Optimisation of the ox- alic acid trickling method and bee tol- erability of different winter treatments: trials in Liebefeld during the last 3 years. The 6th Meeting of the Euro- pean Group for Integrated Varroa Con- trol, 22–23 June 2001, York, United Kingdom, pp. 12–23.
Charrière J.D, Imdorf A, Fluri P (1998) Po- tentiel et limites de l’acide oxalique pour lutter contre Varroa. Rev Suisse Apic. 95: 331–316.
Charrière J-D, Imdorf A (1995) Commentfaire à la recrudescence des Varroa ré- sistants? Rev Suisse Apic. 92: 157–161. Charrière J-D, Imdorf A (2000) Acide oxa- lique par dégouttement: essai 1999/2000 et recommandation d’utilisation pour l’Europe. Rev suisse Apic. 97 (11–12): 400–407.
Charrière J-D, Imdorf A (1999) Nouveau résultats des essais de traitements à l’acide oxalique par dégouttement. Rev Suisse Apic. 96: 368–374.
Charrière J-D, Imdorf A (1998) Méthodes de lutte alternative contre Varroa Rev Suisse Apic. 95: 187–191.
Institut National de Recherche et de Sécurité INRS (2005) Fiche technique, acide oxalique. Paris, France. Available at: www.inrs.fr/default/dms/inrs/FicheToxi- cologique/TI-FT-110/ft110.pdf.
Colin M-E (1999) Intoxications, Bee DiseaseDiagnosis. Options Méditerranéennes.25: 167–175.
Colin M-E (1997) Alternative control of the varroasis. Proceedings of the CIHEAM Seminar on The Varroatoosis in the Mediterranean Region, Granada, 22–23September 1996. Options Méditerra- néennes. 21: 87–98.
Dandeu JP, Lux M, Colin ME, Rabillon J, David B (1991) Étude immuno-chi- mique de l’hémolymphe d’abeille ou- vrière adulte (Apis mellifera L) saine ou infestée par Varroa jacobsoni Oud. Apido. 22: 37–42.
Fluri P, Imdorf A, Charrière J-D (1999) Rubrique Varroa: d’avril à novembre. Rev suisse Apic. 96(3): 45–56.
Giovenazzo P, Dubreuil P (2011) Evaluation of spring organic treatments against Varroa destructor (Acari: Varroidae) in honey bee Apis mellifera (Hyme- noptera: Apidae) colonies in eastern Canada. Exp Appl Acarol. 55(1): 65–76.
Girişgin AO, Aydin E (2010) Efficacies of formic, oxalic and lactic acids against Varroa destructor in naturally infested honeybee Apis mellifera L. colonies in Turkey. Kafkas Univ Vet Fak Derg.16: 941–945.
Gregorc A, Planinc I (2012) Use of thymol formulations, amitraz, and oxalic acid for the control of the varroa mite in honey bee (Apis mellifera carnica) col- onies. J Agric Sci. 56(2): 123–129.
Gregorc A, Pogacnik A, Bowen ID (2004) Cell death in honey bee (Apis mellif- era) larvae treated with oxalic or for- mic acid. Apido. 35: 453–460.
Gregorc A, Planinc I (2001) Acaricidal ef- fect of oxalic acid in honey bee (Apis mellifera) colonies. Apido. 32: 333–340
Hatjina F, Haristos L (2005) Indirect effects of oxalic acid administered by trickling method on honey bee brood. J Agric Res. 44(4): 172–174.
Higes M, Aranzazu M, Suarez M, Liorente J (1999) Negative long-term effects on bee colonies treated with oxalic acid against Varroa jacobsoni. Apido. 30:289–292.
Higes PM (1999) Ecological Varroa Con- trol, notes on control strategies for South Europe. The 5th Meetting of Coordination in Europe of research on integrated control of Varroa mites in honey bee colonies, 13–14 Novembre1999, Merelbeke, Belguim, pp. 63–67. Imdorf A, Charrière J-D (1998) Comment faire la recrudescence de Varroa ré- sistants?. Rev Suisse Apic. 95: 157–161.
Imdorf A, Charrière J-D, Bachofen B (1997) Efficiency checking of the Varroa ja- cobsoni control methods by means of oxalic acid. Apiacta. 32(3): 89–91.
Martin SJ, Highfield AC, Brettell L, Villa- lobos EM, Budge GC, Powell M, Ni- kaido S, Schroeder D C (2012) Global honey bee viral landscape altered by a parasitic mite. Science. 336: 1304–1306.
Mert G, Yucel B (2011) Efficacy levels of organic acids are used for controlling Varroa (Varroa jacobsoni Oudmans) and their effects on colony develop- ment of honey bees (Apis mellifera L.). J Anim Vet Adv. 10(9): 1106–1111.
Moosbeckhofer R (2001) Varroabekämp- fung mit Oxalsäure im Träufelverfah- ren. Bienenvater. 12: 7–12.
Moosbeckhofer R, Pechhacker H, Unter- weger H, Bandion F, Heinrich-Lenz A (2003) Investigations on the oxalic acid content of honey from oxalic acid treat- ed and untreated bee colonies. Eur Food Res Technol. 217: 49–52.
Nanetti A (1999) Oxalic Acid for Mite Con- trol-Result and Review. The 5th Meetting of Coordination in Europe of research on integrated control of Varroa mites in honey bee colonies, 13–14 Novem- ber 1999, Merelbeke, Belguim. pp. 7–14. Nanetti A, Massi S, Mutinelli F, Cremasco S (1995) L’acido ossalico nel controllo
della Varroasi note preliminari. Api- talia. 3: 29–32.
Nanetti A, Stradi G (1997) Varroasi: tratta- mento chimico con acido ossalico in sciroppo zuccherino. L’Ape Nostra Amica. 5: 6–14.
Nasr M.E, Servos D, Bannister R, Wilson G. (2001) Efficacy of three miticides (Ox- alic acid, Formic acid, Apilife Var) on Varroa destructor and Acarapis woodi in honey bee colonies in Canada. Euro- pean Group for Integrated Varroa Con- trol, York.
Nazzi F, Brown S P, Annoscia D, Del Pic- colo F, Di Prisco G, Varricchio P, Del- la Vedova G, Cattonaro F, Caprio E, Pennacchio F (2012) Synergistic para- site-pathogen interactions mediated by host immunity can drive the collapse of honey bee colonies. PLoS Pathog.8(6): 1–16
Nozal MJ, Bernal JL, Gomez LA, Higes M, Meana A (2003) Determination of ox- alic acid and other organic acids in honey and in some anatomic structures of bees, Apido. 34: 181–188.
Tentcheva D, Gauthier L, Jouve S, Canabady- Rochelle L, Dainat B, Cousserans F, Colin ME, Ball BV, Bergoin M (2004) Polymerase chain reaction detection of deformed wing virus (DWV) in Apis mellifera and Varroa destructor. Apido.35: 431–440.
Vandame R (1996) Importance de l’hybridation de l’hôte dans la tolérance à un para- site. Cas de l’acarien Varroa jacobsoni chez les races d’abeilles Apis mellifera européenne et africanisées, en climat tro- pical humide du Mexique. [PhD disse- tation]. Université Claude Bernard, Lyon1, France.
Yang X, Cox-Foster DL (2005) Impact of an ectoparasite on the immunity and pa- thology of an invertebrate: evidence for host immunosuppression and viral am- plification. Proc Nat Acad Sci. 102:7470–7475.
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| Issue | Vol 10 No 4 (2016) | |
| Section | Original Article | |
| Keywords | ||
| Varroa destructor Apis mellifera oxalic acid effectiveness Algeria | ||
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