Treatment of Cutaneous Leishmaniasis in Murine Model by Hydro Alcoholic Essence of Artemisia sieberi

  • A Doroodgar Mail Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Iran
  • M Arbabi Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Iran
  • MR Razavi Department of Parasitology, Pasteur Institute, Tehran, Iran
  • M Mohebali Department of Parasitology, School of Public Health, Tehran University of Medical Sciences, Iran
  • F Sadr Department of Internal Medicine, School of Medicine, Kashan University of Medical Sciences, Iran
Cutaneous leishmaniasis, Leishmania major, Artemisia sieberi, BALB/c,


Background: Considering the prevalence of leishmaniasis in Iran and many side effects associated with pentavalent antimony compounds use in its treatment, this study was designed to evaluate the effect of Artemisia sieberi essence on the experimental ulcers of cutaneous leishmaniasis on BALB/c mice.Methods: This experimental research was performed to determine the effect of various concentrations of  Artemisia essence in BALB/c mice previously infected with active Leishmania major promastigote. A total of 50 infected BALB/c mice were randomly divided into 5 groups. Three groups (30 mice) were used in the experimental condi­tions and the others were assigned as the control groups. The experimental groups received 1%, 3% and 5% of Ar­temisia, respectively. One of the control groups received ethanol 80% and the other received no treatment. The drug was administered by dropping the liquid on the top lesions, three times daily for maximum of 30 d. Every 10 days the ulcers diameter were measured and sampled for amastigote in all groups. Ulcers diameter changes were deter­mined by statistical tests.Results: After 30 days, diameter of CL lesions increased in 1%, 3% and 5% Artemisia concentrations and the control groups. Ulcers got bigger with the more concentration. Treatments could not reduce the diameter or caused small lesions. In addition, the mice direct smears in microscopic studies were positive.Conclusion: To find the effective concentration and the mechanism of the effectiveness of the drug, further investi­gations with less concentrates of A. sieberi essence are recommended.


Arab HA, Rahbari S, Rassouli A, Moslemi MH, Khosravirad F (2006) Determi- nation of artemisinin in Artemisia sieberi and anticoccidial effects of the plant extract in broiler chickens. Trop Anim Health Prod. 38(6): 497-503.

Babaee Khou L, Mohebali M, Niakan Lahiji MR, Mehrabi Tavana A (2007) The therapeutic effect of Eucalyptus, Myr- tus, Artemisia, Allium and urtica ex- tract against cutaneous leishmaniasis by L. major in small white mice (out- bred). J Hakim. 10(2): 21-27.

Baldi A, Dixit VK (2007) Yield enhance- ment strategies for artemisinin pro- duction by suspension cultures of Artemisia annua. Bull Soc Pathol Exot.100(3): 216-7.

Bamoniri A (2004) Constituents of the es- sential oil of Artemisia herba-alba in Kashan area. Iranian J Pharmaceutical. Supplement 2: 83-83.

Berman J (2003) Current treatment approa- ches to leishmaniasis. CUIT Opin In- fect Dis. 16: 397-401.

Farzaneh M, Ahmadzadeh M, Hadian J, Te- hrani AS (2006) Chemical composi- tion and antifungal activity of the es- sential oils of three species of Ar- temisia on some soil-borne phytopa- thogens. Commun Agric Appl Biol Sci.71(3 Pt B):1327-33.

Fata A, Rakltshandeh H, Berenji F, Jalalian- fard A (2006) Treatment of Cutaneous Leishmaniasis in Murine Model by Alcoholic Extract of Berberis vulgaris, Iranian J Parasitol. 1(1): 39-42.

Ghasemi E, Yamini Y, Bahramifar N, Sefidkon F (2007) Comparative analysis of the oil and supercritical CO2 extract of Artemisia sieberi. J Food Engineering.79: 306-311.

Hatimi S, Boudouma M, Bichichi M, Chaib N, Guessous Idrissi N (2000) Evalua- tion in vitro de l’activité antileishma- nienne d’ Artemisia herba-alba Asso. Manuscrit n° 2162. “Thérapeutique”.

Hepburn NC (2001) Management of cutane- ous leishmaniasis, Curr Opin Infect Dis.14(2): 151-154.

Iwu MM, Jackson JE, Schuster BG (1994) Medicinal plants in the fight against leishmaniasis. Parasitol Today.10: 65-68.

Lai H, Sasaki T, Singh NP, Messay A (2005) Effects of artemisinin-tagged holotrans- ferrin on cancer cells. Life Sciences,11(76): 1267-1279.

Markell and Voge M (2006) Medical Para- sitology, Ninth Edition, Saunders El- sevier Press, USA.

Mohebali M (1996) Zoonotic protozoa dis- eases First Edition, Nadi Press, Tehran.

Rowen RJ (2002) Artemisinin: From Ma- laria to Cancer Treatment, Townsend Letter Press, USA.

Rita Bilia A, Lazari D, Messori L, Taglioli V, Temperini C, Vincieri FF (2002) Simple and rapid physico-chemical me- thods to examine action of antimalarial drugs with hemin its application to Artemisia nnua constituents. Life Sci- ences. 7(70): 769-778.

Saddi M, Sanna A, Cottiglia F, Chisu L, Casu L, Bonsignore L, Logu AD (2007) An- tiherpes activity of Artemisia arbores- cens essential oil and inhibition of lat- eral diffusion in Vero cells. Ann Clin Microbiol Antimicrob. 6(1):10.

Service MW. (1996) Medical entomology for students. Chapman and Hall Press, UK.

Sharif M, Ziaei H, Azadbakht M, Daryani A, Ebadattalab A, Rostami M (2006) Effect of Methanolic Extracts of Ar- temisia aucheri and Camellia sinensis on Leishmania major (In Vitro). Turk J Med Sci. 36 (6): 365-369.

Sukul NC, Ghosh S, Sinhababu SP (2005) Reduction in the number of infective Trichinella spiralis larvae in mice by use of homeopathic drugs. Forsch Kom- plementarmed Klass Naturheilkd. 12(4):202-5.

Tyler VE, Brady LR, Robbers JE (1988) Pharmacognosy: Artemisinin and de- rivatives. 9th ed. Lea & Febiger Press, Philidelphia.

Weigel MM, Armijos RX (2003) The tradi- tional and medical treatment of cutane- ous leishmaniasis in rural Ecuador. J Am Acad Dermatol. 48(6): 893-96.

WHO Expertise Committee (1990) Tenth program of world health organization.1st ed. Swiss: WHO press; 27-41

How to Cite
Doroodgar A, Arbabi M, Razavi M, Mohebali M, Sadr F. Treatment of Cutaneous Leishmaniasis in Murine Model by Hydro Alcoholic Essence of Artemisia sieberi. J Arthropod Borne Dis. 2(2):42-47.