Chemical Constitute and Larvicidal Activity of Fractions of Ajuga chamaecistus tomentella Plant against Malaria Vector Anopheles stephensi
Abstract
Backgrand: The genus Ajuga, belongs to Lamiaceae family, is one of the exclusive subspecies in the flora of Iran. The plants of this genus are used traditionally for treatment of joints pain, gout, jaundice, and as insecticide.
Methods: larvicidal activity of methanol 80% extract and partition fractions of hexane, chloroform, and ethyl acetate obtained from aerial parts of Ajuga chamaecistus subspecies tomentella against malaria vector An. stephensi was evaluated. Phytochemical study of active fraction was analyzed using column chromatography and spectroscopy.
Results: According to the results, among different fractions, hexane fraction has the most larvicidal activity with mortality rate of 100% in concentration of 102 ppm and LC50 of 95.66ppm. The structure of compound 1, main phytoecdysteroid compound separated from hexane fraction, was determined to be ajugalide-E.
Conclusion: The results suggested that the hexane fraction of Ajuga chamaecistus subsp tomentella could be used as a natural and biodegradable insecticide.
Beckage NE, Marion KM, Walton WE, Wirth MC, Tan FF (2004) Compara- tive larvicidal toxicities of three ecdy- sone Agonists on the mosquitoes Ae- des aegypti, Culex quinquefasciatus, and Anopheles gambiae. Arch Insect Physiol. 57(3): 111–122.
Ben Jannet H, Harzallah-Skhiri F, Mighri Z, Simmonds MS, Blaney WM (2000) Responses of Spodoptera littoralis lar- vae to Tunisian plant extracts and to neo-clerodane diterpenoids isolated from Ajuga pseudoiva leaves. Fitoterapia.71(2): 105–112.
Ben Jannet H, H-Skhiri F, Mighri Z, Simmonds MSJ, Blaney WM (2001) Antifeedant activity of plant extracts and of new natural diglyceride com- pounds isolated from Ajuga pseudoiva leaves against Spodoptera littoralis larvae. Ind Crops Prod. 14(3): 213–222.
Bondì ML, Al-Hillo MR, Lamara K, Ladjel S, Bruno M, Piozzi F, Simmonds MS (2000) Occurrence of the antifeedant
, 15-dihydroajugapitin in the aerial parts of Ajuga iva from Algeria. Biochem Sys Eco. 28(10): 1023–1025.
Chan YY, Wu TS, Kuoh CS, Damu AG (2005) A New Phytoecdysteroid from Ajuga taiwanensis. Chem Pharm Bull.
(7): 836–838.
Doosti S, Azari-Hamidian S, Vatandoost H, Oshaghi MA, Hosseini S (2006) Tax- onomic differentiation of Anopheles sacharovi and An. maculipennis s.l. (Diptera: Culicidae) larvae by seta 2 (antepalmate hair). Acta Medica Iran- ica. 44(1): 21–27.
Edrissian GhH (2006) Malaria in Iran: Past and Present Situation. Iranian J Para- sitol. 1(1): 1–14.
Finney DJ (1971) Probit Analysis. 3rd ed.London: Cambridge University Press, p. 14.
Gautam R, Jachak SM, Saklani A (2011) Anti-inflammatory effect of Ajuga bracteosa Wall ex Benth. mediated through cyclooxygenase (COX) inhi- bition. J Ethnophar. 133: 928–930.
Govindarajan M, Mathivanan T, Elumalai K, Krishnappa, K, Anandan A (2011) Mos- quito larvicidal, ovicidal, and repellent properties of botanical extracts against Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Cu- licidae). Parasitol Res. 109: 353–367.
Hadjiakhoondi A, Sadeghipour-Roodsari HR, Vatandoost H, Khanavi M, Abai MR, Vosoughi M, Kazemi M, Abai MR (2006) Fatty acid composition and tox- icity of Melia azedarach L. fruits against malaria vector Anopheles stephensi. Iran J Pharm Sci. 2(2): 97–102.
Hadjiakhoondi A, Vatandoost H, Jamshidi A, Bagherj Amiri E (2003) Chemical constituents and efficacy of Cymbopo- gon olivieri (Boiss) bar essential oil against malaria vector, Anopheles ste- phensi. Daru. 11(3): 125–128
Hilaly JE, Lyoussi B (2002) Hypoglycemic effect of lyophilized total water extract of Ajuga iva in normal and strepto- zotocin diabetic rats. J Ethnophar. 80:109–113.
Israili HZ, Lyoussi B (2009) Ethnopharma- cology of the plants of genus Ajuga. Pak J Pharm Sci. 22: 425–462.
Jorjani MH. Zakhire Kharazmshahi (Kha- razmid reservoir) (2012) Qom: Insti- tute of Natural Medicine Resurgence Press. 3: 180.
Khanavi M, Bagheri Toulabi P, Abai MR, Sadati N, Hadjiakhoondi F, Hadjiakhoon- di A, Vatandoost H (2011) Larvicidal activity of marine algae, Sargassum swartzii and Chondria dasyphylla, against malaria vector, Anopheles ste- phensi. J Vector Borne Dis. 48: 241–244.
Kuria KAM, Coster S, Muriuki G, Masengo W, Kibwage I, Hoogmartens J, Laeke- man GM. (2001) Antimalarial activity of Ajuga remota Benth (labiatae) and Caesalpinia volkensii Harms (Caesal- piniaceae): in vitro confirmation of eth- nopharmacological use. J Ethnophar.74: 141–148.
Manjari MS, Karthi S, Ramkumar G, Muthusamy R, Natarajan D, Shivakumar MS (2014) Chemical composition and larvicidal activity of plant extracts from Clausena dentata (Willd) (Rutaceae) against dengue, malaria, and filariasis vectors. Parasitol Res. 113: 2475–2481.
Mozaffarian V (2007) A ◌◌Dictionary of Iranian Plant Names. 5rd ed. Farhang moaser Press, Tehran.
Naghibi F, Mosaddegh M, Mohammadi Motamed S, Ghorbani A (2005) Labi- atae family in folk medicine in Iran: from ethnobotany to pharmacology. Iran J Pharm Res. 2:63–79.
Nyamoita MG, Ester I, Zakaria MH, Wilber L, Ochola BJ, Ahmed H (2013) Larvi- cidal and Brine Shrimp Activities of Vitex Schiliebenii Extracts and Isolated Phytoecdysteroids on Anopheles gam- biae Giles S.S Larvae. J App Pharm Sci. 3(05): 091–095.
Ono Y, Fukaya Y, Imai S, Yamakuni T (2008) Beneficial effects of Ajuga de- cumbens on osteoprosis and arthritis. Biol Pharm Bull. 31: 1199–1204.
Ramazanov N Sh (2005) Phytoecdysteroids and other biologically active com- pounds from plants of genus Ajuga. Chem Nat Compd. 41: 361–369.
harrabe K, Sayah F, LaFont R (2010) Die- tary effects of four phytoecdysteroids on growth and development of the In- dian meal moth, Plodia interpunctella. J Insect Sci. 10: 13.
Sadati N, Ostad SN, Karimian Z, Shams Ar- dekani MR, Akbarzadeh T, Hadjiakhoon- di A, Khanavi M (2012a) Phytochemi- cal study and in vitro cytotoxic effect of Ajuga chamaecistus ssp. tomentella. Asian J Chem. 24: 2871–2874.
Sadati N, Jenett-Siems K, Siems K, Shams Ardekani MR, Hadjiakhoondi A, Ak- barzadeh T, Ostad SN, Khanavi M (2012b) Major constituents and cyto- toxic effects of Ajuga chamaecistus subsp. tomentella. Z Naturforsch. 67:275–281.
Sedaghat NM, Sanei Dehkordi A, Abai MR, Khanavi M, Mohtarami F, Salim Ab- adi Y, Rafi F, Vatandoost H (2011) Larvicidal Activity of Essential Oils of Apiaceae Plants against Malaria Vec- tor, Anopheles stephensi. Iran J Ar- thropod-Borne Dis. 5(2): 51–59.
Sharma P, Mohan L, Srivastava CN (2004) Larval susceptibility of Ajuga remota against anopheline and culicine mos- quitos. Southeast Asian J Trop Med Public Health. 35(3): 608–610.
Vatandoost H, Moinvaziri VM (2004) Larvi- cidal activity of neem tree extract (Neemarin) against mosquito larvae in the Islamic Republic of Iran. Eastern Med Health J. 10: 573–578.
| Files | ||
| Issue | Vol 11 No 1 (2017) | |
| Section | Original Article | |
| Keywords | ||
| Ajuga chamaecistus subspecies tomentella Larvicidal Anopheles stephensi phytoecdysteroid | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
