Assessing the Ovarian Accessory Glands to Determine the Parity of Phlebotomus papatasi, Vector of Zoonotic Cutaneous Leishmaniasis, under Laboratory Condition
AbstractBackground: Zoonotic cutaneous leishmaniasis (ZCL) is a neglected tropical disease prevailed in many rural areas of 17 out of 31 provinces in Iran. The main vector of the disease is Phlebotomus papatasi and the causative agent is Leishmania major in ZCL foci of Iran. In the current study we investigated the validity of accessory glands secretions as an indicator to recognize parous from nulliparous Ph. papatasi females under laboratory conditions.Methods: Over 235 laboratory-reared females of Ph. papatasi were dissected in 6 groups including: newly emerged, one hour, one day, two days, and three days after blood feeding and also after oviposition under stereo microscope for their parity in 2014–2015.Results: Transparent glands were compatible with nulliparous only in newly emerged sand flies. In sand flies dissected after oviposition, accessory glands were rather large as a result of oviposition though they were transparent.Conclusion: The accessory glands secretions could not be as an indicator for distinguishing parous from nulliparous of Ph. papatasi females.
Adler S, Theodor O (1935) I Investigations on Mediterranean Kala Azar. Viii.-- further Observations on Mediterranean Sandflies, Proc R Soc Lond B Biol Sci.116(801): 505–515.
Akhavan AA, Yaghoobi-Ershadi MR, Khame- sipour A, Mirhendi H, Alimohammadian MH, Rassi Y, Arandian MH, Jafari R, Abdoli H, Shareghi N, Ghanei M, Jalali- zand N (2010) Dynamics of Leishmania infection rates in Rhombomys opimus (Rodentia: Gerbillinae) population of an
endemic focus of zoonotic cutaneous leishmaniasis in Iran. Bull Soc Pathol Exot. 103(2): 84–89.
Chaniotis BN, Anderson JA (1967) Age struc- ture, population dynamics and vector potential of Phlebotomus in North Cal- ifornia. Part I: Distinguishing parous from nuliparous flies. J Med Entomol.4: 251–254.
Dejeux P (1991) Information on the epide- miology and control of the leishmania- sis, by country or territory. World Health Organ, Geneva.
Detinova TS (1962) Age-grouping methods in Diptera of medical importance. Wld Hlth Org. 47: 216.
Hashiguchi Y (1987) Studies on New world leishmaniasis and its transmission, with particular reference to Ecuador, Kyowa printing and Ltd, Kochi. 174.
Killick-Kendrick M, Killick-Kendrick R (1991) The initial establishment of sand fly colonies. Parassitologia. 33: 313–320.
Lewis DJ (1965) Internal structural features of some central American phlebotom- ine sandflies. Ann Trop Med Parasit.54: 351–365.
Lewis DJ, Lainson R, Shaw JJ (1970) De- termination of parous rates in Phlebotom- ine sandflies with special reference to Amazonian species. Bull Entomol Res.60: 209–219.
Lewis DJ, Minter DM (1960) Internal structural changes in some African Phlebotominae. Ann Trop Med Parasitol. 54: 351–365.
Modi GB, Tesh RB (1983) A simple tech- nique for mass rearing Lutzomyia Long- ipalpis and Phlebotomus papatasi (Dip- tera: Psychodidae) in the laboratory. J Med Entomol. 20: 568–569.
Mohebali M, Javadian E, Yaghoobi-Ershadi MR, Akhavan AA, Hajjaran H, Abaei MR, (2004) Charactrization of leish- mania infection in rodents from en- demic areas of Islamic Republic of Iran.
East Mediterr Health J. 10: 591–599. Ready PD, Lainson R, Wilkes TJ, Killick-
Kendrick R (1984) On the accuracy of age-grading neotropical phlebotomines by counting follicular dilatations: first laboratory experiments, using colonies of Lutzomyia flaviscutellata (Mangabei- ra) and L. furcata (Mangabeira) (Dip- tera: Psychodidae). Bull Entomol Res.74: 641–646.
Scorza JV, Ortiz I, Gomez I (1968) Obser- vationes biologicas sobre algunos fleboto- mos de Rancho Grande (Venezuela). Acta Boil Venez. 6: 52–65 (In Spanish).
Shirzadi M (2010) Guideline for control of cutaneous leishmaniasis. Department of Zoonosis, CDC, Ministry of Health and Medical Education, Tehran, Iran.
Takaoka H, Gomez E, Alexander J, Hashigu- chi Y (1989) Observations on the validity of the ovarian accessory glands of seven ecuadorian sand fly species (Diptera: Psy- chodidae) in determining their parity. Ja- pan J Trop Med Hyg. 17(2): 149–155.
WHO (2010) Control of the leishmaniases: report of a meeting of the WHO Expert Commitee on the Control of Leish- maniases, Geneva, 22–26 March 2010. Yaghoobi-Ershadi MR, Javadian E, Tahvil- dare-Bidruni Gh (1994) The isolation
of Leishmanha major from Phlebotomus (paraphleboyomus) caucasicus,in Isfahan Province, Islamic Republic of Iran. Trans R Soc Trop Med Hyg. 88: 518–519.
Yaghoobi-Ershadi MR, Akhavan AA, Mohe- bali M (1996) Meriones libycus and Rhombomys opimus (Rodentia: gerbilli- dae) are the main reservoir hosts in a new focus of zoonotic cutaneous leish- maniasis in Iran. Trans R Soc Trop Med Hyg. 90: 503–504.
Young DG, Perkins PV, Endris RG (1981) A larval diet for rearing phlebotomine sand- flies (Diptera: Psychodidae). J Med En- tomol. 18(5): 446.
|Issue||Vol 11 No 1 (2017)|
|Phlebotomus papatasi Accessory glands Parous Nulliparous Parity|
|Rights and permissions|
|This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.|