Laboratory Study on Biological Control of Ticks (Acari: Ixodidae) by Entomopathogenic Indigenous Fungi (Beauveria bassiana)


Background: Chemical control method using different acaricides as spray, dipping solution or pour-on is routinely used for controlling ticks. Biological control agents are favorable due to their safety for animals and environment. Entomopathogenic fungi such as Beauveria bassiana are well known for controlling ticks. In this study, two Iranian indigenous strains of B. bassiana (B. bassiana 5197 and B. bassiana Evin) were selected and grown on specific me­dia. The pathogenic effects of these strains were evaluated on adult stages of two Iranian Ixodidae members (H. anatolicum anatolicum Koch 1844, and H. marginatum Koch 1844) by dipping method.Methods: Two Iranian strains of Beauveria bassiana (Beauveria bassiana 5197 and Beauveria bassiana Evin) were selected and were grown successfully on specific media. The pathogenic effects of these strains were evaluated on adult stages of Iranian Ixodidae members such as, Hyalomma anatolicum anatolicum and H. marginatum by dipping method (these ticks were grown up at laboratory conditions during 2002 up to 2003 and still it is continued) .Results: There was no effect of strain 5197 on mortality or fecundity rates for ticks. There was acute phase sign of paralysis in test group after dipping ticks in suspension made from Evin strain of B. bassiana. In addition, the test groups were totally died after four months, but the control groups survived for six months.Conclusion: High concentration of fungal spores is needed for inducing fungal infection. Additional study using different strains and fungi on Iranian ticks is proposed. 

Benjamin MA, Zhioua E, Ostfeld RS (2002) Laboratory and field evaluation of the entomopathogenic fungus Metarhizium anisopliae (Deuteromycetes) for con- trolling questing adult Ixodes scapu- laris (Acari: ixodidae). J Med Entomol. (39): 723-728.

Bittencourt V, Souza EJ, Peralva S, Mas- carenhas A (1997) Evaluation of the in vitro efficacy of two isolates of the en- tomopathogenic fungus Beauveria bas- siana (Bals.) vuill. In engorged females of Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae). Revista Brasileira de Parasitologia Veterinaria. 6(1): 49-52.

Camacho ER, Navaro G, Rodriguez RM, Marillo EY (1998) Effectiveness of Verticillium lecanii against the parasitic stage of the tick Boophilus microplus (Acari: Metastigmata: Ixodidae). Revista- Colombiana- de- Entomologia 24(1-2):67-69.

Correia AD, Fiorin A, Monteiro AC, Verissimo C (1998) Effects of Metarhizium ani- sopliae on the tick Boophilus micro- plus (Acari: Ixodidae) in stabled cattle. J Inv Pathol. 71(2) 189-191.

Cradock KR (2005) Interactions of Beauve- ria bassiana with the American dog tick, Dermacentor variabilis (Say), And the lone star tick, Amblyomma ameri- canum L. [PhD Dissertation]. The Ohio State University, USA.

Ferron P (1978) Biological Control of insect pests by entomopathogenic fungi. Annual Review of Entomology. 23: 409-442.

Frazzon AP, Da Silva Vaz Jr, Masuda A,Schrank A, Vainstein MH (2000) In vitro assessment of Metarhizium ani- sopliae isolates to control the cattle tick Boophilus microplus Veterinary Para- sitology. 94: 117-125.

Frolov BA (1974) Chemical and Biological methods of controlling poultry ectopa- rasites. Veterinary Moscow. 12: 66-68.

Kaaya GP, Hassan S (2000) Entomopathoge nous fungi as promising biopesticides for tick control. Experimental and Ap- plied Acarology. 24: 913-926.

Kaaya GP (2000) Laboratory and field evalua- tion of entomopathogenous fungi for tick control. Annals of the New York Academy of Science.916: 559-564.

Lopez G, Marin H, Londono M, Vahos R (1998) Utilization of Metarhizium ani- sopliae and Beauveria bassiana for the biological control of the tick Boophilus microplus. Noticampo. 10: 12-14.

McCoy CW, Samson RA, Boucias DG (1988) Entomogenous fungi. In: Ignoffo CM (Ed.). CRC Handbook of Natural Pesti- cides. Microbial Insecticides. Part A: Entomogenous protozoa and fungi Vol 5. CRC Press, Boca Raton, USA, pp.151-236.

Mwangi E, Kaaya G, Essuman S (1995) Ex- perimental infections of the tick Rhi- picephalus appendiculatus with ento- mopathogenic fungi, Beauveria bas- siana and Metarhizium anisopliae and natural infections of some ticks with bacteria and fungi. Journal of African Zoology. 109(2): 151-160.

Rijo E (1998) Biological control of ticks with entomopathogenic fungi. Rivista Pecuaria de Nicaragua. 22: 17-18.

Samish M, Rehaacek J (1999) Pathogens and predators of ticks and their potential in biological control. Annual Review of Entomology. 44: 159-182.

Samish M, Gindin G, Alekseev E, Glazer I (2001) Pathogenicity of entomopatho- genic fungi to different developmental stages of Rhipicephalus sanguineus (Acari: Ixodidae). J Parasitol. (87): 1355-1359.

Tanada Y, Kaya HK (1993) Insect pathology.Academic Press. Inc., San Diego.

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Biological control fungi Beauveria bassiana ticks Ixodidae Iran

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How to Cite
Abdigoudarzi M, Esmaeilnia K, Shariat N. Laboratory Study on Biological Control of Ticks (Acari: Ixodidae) by Entomopathogenic Indigenous Fungi (Beauveria bassiana). J Arthropod Borne Dis. 1;3(2):36-43.