The Seminested PCR Based Detection of Leishmania infantum Infection in Asymptomatic Dogs in a New Endemic Focus of Visceral Leishmaniasis in Iran
AbstractVisceral Leishmaniasis (Kala-azar) is a serious health problem in some northern and south western parts of Iran. The incidence of kala-azar caused by Leishmania infantum has recently increased in Nourabad-Mamassani district of Fars Province, in the south of the country. This study was designed to determine the role of asymptomatic dogs as host reservoir of L. infantum in this new formed focus and detection of prevalence of infection near them. A total of 20 as¬ymptomatic stray and sheep dogs were randomly sampled. The Buffy coat layer of their peripheral blood was used for DNA extraction and PCR. A species specific seminested PCR was used for DNA amplification using LINR4, LIN17 and LIN19 primers. These primers amplified variable area of the minicircle kDNA of Leishmania parasites. Of the 20 sampled dogs checked for leishmanial kDNA, six (30%) were found naturally infected. It is concluded that, dogs (Canis familiaris) even if asympto¬matic, is considered as the domestic host reservoir of kala-azar in this endemic focus.
Aransay AM, Scoulica E, Tselentis Y (2000) Detection and identification of Leishmania DNA within naturally infected sandflies by semi-nested PCR on minicircle kineto- plast DNA. Applied Environ Microbiol.66: 1933-1938.
Ardehali S (2000) Characterization of Leishma- nia isolated in Iran: serotyping with spe- cies specific monoclonal antibodies. Acta Trop. 75: 301-307.
Azizi K, Rassi Y, Javadian E, Motazedian MH, Rafizadeh S, Yaghoobi Ershadi MR, Mo- hebali M (2006) Phlebotomus (Paraphle- botomus) alexandri: a probable vector of Leishmania infantum in Iran. Ann Trop Med Parasitol. 100(1): 63-68.
Edrissian GH, Nadim A, Alborzi AV, Ardehali S (1999) Visceral leishmaniasis: the Ira- nian experiences. Arch Iran Med. 1:22-26.
Edrissian GH, Ahanchin AR, Gharachahi AM (1993) Seroepidemiological studies of visceral leishmaniasis and search for ani- mal reservoirs in Fars province, southern Iran. Iranian J Med Sci. 18: 99-105.
Brewster S, Aslett M, Barker DC (1998) Ki- netoplast DNA minicircle database. Para- sitol Today. 14: 437-438.
Hamidi AN, Nadim A, Edrissian GH, Tahvil- dari-Bidrouni G, Javadian E (1982) Vis- ceral leishmaniasis of jackals and dogs in northern Iran. Trans Roy Soc Trop Med Hyg. 76: 756-757.
Kato H, Uezato H, Katakura K, Marco M, Barroso J, Gomez P, Mimori E, Korenaga T, Iwata M, Nonaka H, Hashiguchi Y (2005) Detection and identification of Leishmania species within naturally in- fected sandflies in the Andean areas of Ecuador by a polymerase chain reaction. Am J Trop Med Hyg. 72(1): 87-93.
Lachaud L, Marchergui-Hammami S, Chabbert E, Dereure J, Dedet J, Bastien P (2002) Comparison of six methods using periph- eral blood for detection of canine visceral leishmaniasis. J Clinic Microbiol. 40(1):210-215.
Le Fichoux Y, Quaranta J, Aufeuvre J, Lelievre A, Marty P, Suffia I, Rousseau D, Kubar J (1999) Occurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endimicity in southern France. J Clinic Microbiol. 37(6): 1953-1957.
Mazloumi Gavgani S, Mohite H, Edrissian GH, Mohebali M, Davies C (2002) Domestic dog ownership in Iran is a risk factor for human infection with Leishmania infan- tum. Am J Trop Med Hyg. 67(5): 511-515.
Mohebali M, Hajjaran H, Hamzavi Y, Mobedi I, Arshi S, Zarei Z, Akhoundi B, Manouche- hri K, Avizeh R, Fakhar M (2005) Epide- miological aspects of canine visceral leishmaniasis in the Islamic Republic of Iran. Vet Parasitol. 129: 243-251.
Mohebali M, Hamzavi Y, Edrissian GH, Forouzani A (2001) Seroepidemiological study of visceral leishmaniasis among humans and animal reservoirs in Bushehr prov- ince, Islamic republic of Iran. East Mediterr Health J. 7: 912-917.
Mohebali M, Poormohammadi B, Kanani A, Hajjaran H, Edrissian GH (1998) Ro- dents: another group of animal hosts of vis- ceral leishmaniasis in Meshkin-Shahr district, Islamic Republic of Iran. Iranian J Publ Health. 4(2): 376-378.
Motazedian MH, Karamian M, Noyes HA, Ardehali S (2002) DNA extraction and am- plification of Leishmania from archieved, Giemsa stained slides for the diagnosis of cutaneous leishmaniasis by PCR. Ann Trop Med Parasitol. 96: 31-34.
Nadim A, Hamidi N, Javadian E, Tahvildari Bidrouni E, Amini H (1978) Present status of kala-azar in Iran. Am J Trop Med Hyg.27(1): 25-28.
Peters W, Killick-Kendrick R (1987). Leishma- ni- asis in Biology and Medicine. Academic Press, New York.
Rassi Y, Javadian E, Nadim A, Zahraii A, Vatandoost H, Motazedian MH, Azizi K, Mohebali M (2005) Phlebotomus (Lar- roussius) kandelakii the principal and proven vector of visceral leishmaniasis in north west of Iran. Pak J Biol Sci. 8(12):1802-1806.
World Health Organization (1990) Control of the leishmaniasis. Report of a WHO ex- pert committee. Technical Report Series No. 793. WHO, Geneva.
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