Original Article

The Effects of Isolated Fractions of Mesobuthus eupeus Scorpion Venom on Humoral Immune Response

Abstract

Background: Many elements such as immunosuppressive, chemotactic and anti-inflammatory peptide that could effect on human and animals physiologic system were determined in venom. This study evaluated the use of Meso­buthus eupeus scorpion venom fractions as an immunomodulator.
Methods:
The venom fractions collected from Khuzestan Province in South West of Iran were purified by ion ex­change chromatography. Elution of the bounded elements was done by using a linear gradient of sodium chloride (0.1, 0.25, 0.5, 0.75, 1, 1.25, 1.5 and 2 molar). The fractions were analyzed by Bradford spectrophotometric and SDS-PAGE method. After treatments of chicken with venom fractions and sheep red blood cell (SRBC), direct hae­magglutination test in microtiter plate was used for the determination of the chicken SRBC antibody titer.
Results:
The fraction released by NaCl 1.25M had the highest protein concentration. The highest and lowest anti­body titer was determined at the fifth (NaCl 0.75 molar) and seventh fraction (NaCl 1.25 molar), respectively.
Conclusion:
Different protein profile of isolated fractions, were associated with various effect on immune response. Both enhancing and suppressing of the chicken humoral immune response to SRBC were observed after M. eupeus faction’s venom treatment. It is due to biological functions of venom components. Purification of these elements would provide the new agents for immune responses manipulation.

Kim KS, Cho HS, Lee SD, Kim KH, Cho JY, Chung KH, Lee YC, Moon SK, Kim CH (2005) Inhibitory effect of Buthus martensi Karsch extracts on in¬terleukin-1betainduced expres¬sion of nitric oxide (NO) synthase and produc¬tion of NO in human chon-drocytes and LPS-induced NO and prostaglandin E2 production in mouse peritoneal macrophages. Tox-icol In Vitro. 19: 757–769.

Boyer LV, Theodorou AA, Berg RA, Mallie J (2009) Antivenom for criti-cally ill children with neurotoxicity from scorpion sting. N Engl J Med. 360: 2090–2098.

Karatas A (2003) Mesobuthus eupeus (Koch, 1839) (Scorpiones: Buthidae) in An-atolia. Euscorpius 7: 1–7.

Petricevich VL (2010) Scorpion Venom and the Inflammatory Re¬sponse. Mediators Inflamm. 2010: 903295.

Voronov E, Apte RN, Sofer S (1999) The systemic inflammatory response syndrome related to the release of cy¬to¬kines following severe enven-omation. J Venom Anim Toxins. 5: 5–33.

Possani LD, Merino E, Corona M, Boli-var F, Becerril B (2000) Peptides and genes coding for scorpion toxins that affect ion-channels. Biochimie. 82: 861–868.

Dani MP, Richards EH (2010) Iden¬ti¬fi-cation, cloning and expression of a sec-ond gene (vpr1) from the venom of the endoparasitic wasp, Pimpla hypo¬chon¬driaca that displays immu-no¬suppres¬sive activity. J Insect Phys¬iol. 56: 195–203.

Souza BM, Mendes MA, Santos LD, Marques MR, Cesar LM, Almeida RN, Pagnocca FC, Konno K, Palma MS (2005) Structural and functional char¬acterization of two novel peptide toxins isolated from the venom of the social wasp Polybia paulista. Pep-tides. 26: 2157–2164.

Baptista-Saidemberg NB, Saidem¬berg DM, de Souza BM, Cesar-Tognoli LM, Fer¬reira VM, Mendes MA, Cabrera MP, Ruggiero NJ, Palma MS (2010) Pro¬tonectin (1–6): a nov-el chemotactic peptide from the venom of the social was Agelaia pallipes pallipes. Toxicon. 56: 880–889.

Park JH, Kim KH, Lee WR, Han SM, Park KK (2012) Protective ef¬fect of melittin on inflammation and apoptosis in acute liver failure. Apoptosis. 17: 61–69.

Hmed BN, Serria HT, Mounir ZK (2013) Scorpion peptides: potential use for new drug development. J Toxicol. 2013: 958797.

Ortiz E, Gurrola GB, Schwartz EF, Pos-sani LD (2015) Scorpion venom com¬ponents as potential candidates for drug development. Toxicon. 93: 125–135.

Wang Z, Wang W, Shao Z, Gao B, Li J, Ma J, Li J, Che H, Zhang W (2009) Eukaryotic expression and purifica-tion of anti-epilepsy peptide of Bu-thus martensii Karsch and its protein interactions. Mol Cell Bio¬chem. 330 (1–2): 97–104.

Ahmadi M, Zare-Mirakabadi A, Hashemlou M, Hejazi M (2009) Study on anti in¬flammatory effect of scorpion (Mesobuthus eupeus) ven¬om in ad-juvant-induced arthritis in rats. Arch Ra-zi Inst. 64: 51–56.

Razi Jalali M, Jalali MT, Mapar Z (2015) Evaluation of plasma cyto-kine levels in Mesobothus eupeus (Scorpionida: Buthidae) scorpion en-venomation in rats treated with poly-valent antivenom. Jundishapur J Health Sci. 7(1): e27159.

Laemmli K (1970) Cleavage of struc-tural proteins during the assembly of the head of bacteriophage T4. Na-ture. 227: 680–685.

Ladics GS (2007) Use of SRBC anti¬body responses for immunotoxicity test-ing. Methods. 41: 9–19.

Fatani AJ, Furman BL, Zeitlin IJ (1998) The involvement of plasma kinins in the cardiovascular effects of Leiurus quinquestriatus scorpion venom in anes¬thetized rabbits. Toxi¬con 36: 523–536.

Kini RM (2003) Excitement ahead: struc¬ture, function and mechanism of snake venom phospholipase A2 en¬zymes. Tox¬icon. 42: 827–840.

Vogel CW, Fritzinger DC (2010) Cobra

venom factor: structure, func¬tion and humanization for therapeutic comple¬ment depletion. Toxicon. 56: 1198–1222.

Meki AR, Mohey El-Dean ZM (1998) Serum interleukin-1B, inter¬leukin-6, nitric oxide and alpha 1-antitrypsin in scorpion envenomed children. Toxicon. 36: 1851–1859.

Fukuhara YDM, Reis M, Dellalibera-Joviliano R, Cunha FQC, Donadi EA (2003) Increased plasma levels of IL-1β, IL-6, IL-8, IL-10 and TNF-α in patients moderately or severely en¬venomed by Tityus serrulatus scor¬pion sting. Toxicon. 41: 49–55.

Kou JQ, Han R, Xu YL, Ding XL, Wang SZ, Chen CX, Ji HZ, Ding ZH, Qin ZH (2014) Differential ef¬fects of Naja naja atra venom on im¬mune ac-tivity. Evid Based Comple¬ment Al-ternat Med. 2014: 287631.

Petricevich VL, Teixeira CFP, Tam¬bourgi DV, Gutierrez JM (2000) In¬crements in serum cytokine and nitric oxide levels in mice injected with Both-rops asper and Bothrops ja¬raraca snake venoms. Toxicon. 38: 1253–1266.

Hadaddezfuli R, Khodadadi A, As¬sare-hzadegan MA, Pipelzadeh MH, Saa-di S (2015) Hemiscorpius lepturus ven¬om induces expression and pro-duction of interleukin-12 in human monocytes. Toxicon. 100: 27–31.

Saadi S, Assarehzadegan MA, Pipelza-deh MH, Hadaddezfuli R (2015) In-duction of IL-12 from hu¬man mono-cytes after stimulation with An-drocto¬nus crassicauda scor¬pion venom. Tox¬icon. 106: 117–121.

Sousa-e-Silva MCC, Gonçalves LRC, Mariano M (1996) The venom of South American rattlesnakes in¬hibits mac-rophage functions and is endowed with anti-inflammatory properties. Media-

tors Inflamm. 5(1): 18–23.

Cardoso DF, Mota I (1997) Effect of Crotalus durissus terrificus venom on the humoral and cellular immune re¬sponse. Toxicon. 35: 607–612.

Mirshafiey A (2007) Venom therapy in multiple sclerosis. Neuropharma¬cology. 53: 353–361.

Shi GN, Liu YL, Lin HM, Yang SL, Feng YL, Reid PF, Qin ZH (2011) Involvement of cholinergic system in suppression of formalin-induced in¬flammatory pain by cobratoxin. Acta Pharmacol Sin. 32: 1233–1238.

Park MH, Song HS, Kim KH, Son DJ, Lee SH, Yoon DY, Kim Y, Park IY, Song S, Hwang BY, Jung JK, Hong JT (2005) Cobrotoxin inhibits NF-κB activation and target gene ex-pression through reaction with NF-κB signal molecules. Biochemistry. 44(23): 8326–8336.

Petricevich VL (2006) Balance be¬tween pro- and anti-inflammatory cy-tokines in mice treated with Centru-roides nox¬ius scorpion venom. Me-di¬ators Inflamm. 2006(6): 54273.

Jalali A, Pipelzadeh MH, Taraz M, Khoda-dadi A, Makvandi M, Rowan EG (2011) Serum TNF-alpha levels re-flect the clinical severity of enven-omation following a Hemiscorpius lep-turus sting. Eur Cytokine Netw. 22: 5–10.

Luna KPO, Melo C, Magalhães V, Mar-tins-Filho OA, Pereira VR (2011) Both-rops erythromelas snake venom in-duces a proinflammatory response in mice splenocytes. Int J Interf Cytok Mediator Res. 2011(3): 9–18.

Casella-Martins A, Ayres LR, Burin SM, Morais FR, Pereira JC, Faccioli LH, Sampaio SV, Arantes EC, Cas-tro FA, Pereira-Crott LS (2015) Im-munomodu¬latory activity of Tityus serrulatus scor¬pion venom on humanT lymphocytes. J Venom Anim Toxins Incl Trop Dis. 21: 46.

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SectionOriginal Article
Keywords
Venom Mesobuthus eupeus Immune response

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How to Cite
1.
Khosravi M, Mayahi M, Kaviani F, Nemati M. The Effects of Isolated Fractions of Mesobuthus eupeus Scorpion Venom on Humoral Immune Response. J Arthropod Borne Dis. 2017;11(4):497-503.