High Resistance of Vector of West Nile Virus, Culex pipiens Linnaeus (Diptera: Culicidae) to Different Insecticides Recommended by WHO in Northern Iran
Background: Culex pipiens is a mosquito species distributed in different parts of Iran. It is known as vector of some diseases as well as a nuisance insect. A successful control campaign needs to study the biology, ecology and susceptibility status of the target pest. The aim of this study was to investigate the susceptibility status of Culex pipiens to some insecticides recommended by WHO in north of Iran.
Methods: Larvae were collected from different breeding places in Sari County by standard dipping method. They were reared to adult stage in an under standard condition. World Health Organization (WHO) standard test kits and the diagnostic dose of 12 imagicides were used for adults, while two larvicides were used against larvae.
Results: The least and highest mortality rates after exposure to insecticides were 4.7% (Propoxur 0.1%) and 76.4% (Etofenprox 0.5%), respectively. Larvae showed high resistance to temephos (19.19%) and malathion (54.63%). Culex pipiens was found to be resistant to all used insecticides/larvicides at diagnostic dose.
Conclusion: Intensive use of pesticides against agriculture and urban pests increased the resistance level of this species to different insecticides, although currently there is no control program against it. Study on the mechanisms of resistant in this species to different insecticides is recommended.
Becker N, Petrić D, Boase C, Lane J, Zgomba M, Dahl C, Kaiser A (2003) Mosquitoes and their control. New York: Springer, p. 577.
Vinogradova EB (2000) Culex pipiens pipiens mosquitoes: taxonomy, distri¬bution, ecology, physiology, genetics, applied importance and control. Pen¬soft Publishers, p. 253.
Meshkat Z, Chinikar S, Shakeri M, Ma¬navi¬far L, Moradi M, Mirshahabi H, Jalali T, Khakifirouz S, Shahhosseini N (2015) Prev¬alence of West Nile vi¬rus in Mash¬had, Iran: A population-based study. Asian Pac J Trop Med. 8(3): 203–205.
Bagheri M, Terenius O, Oshaghi MA, Mota¬zakker M, Asgari S, Dabiri F, Vatan¬doost H, Mohammadi Bavani M, Chavshin AR (2015) West Nile Virus in mosquitoes of Iranian Wet¬lands. Vec¬tor-Borne Zoon Dis. 15(12): 750–754.
Aghaie A, Aaskov J, Chinikar S, Niedrig M, Banazadeh S, Mohammadpour HK (2016) Frequency of West Nile virus infection in Iranian blood do-nors. In¬dian J Hematol Blood Trans. 32(3): 343–346.
Azari-Hamidian SH, Yaghoobi-Ershadi MR, Javadian E, Mobedi I, Abai MR (2007) Review of dirofilariasis in Iran. J Gui¬lan Univ Med Sci. 15(60): 102–114.
Azari-Hamidian SH, Yaghoobi-Ershadi MR, Javadian E, Abai MR, Mobedi I, Lin-ton YM, Harbach RE (2009) Dis-tribution and ecology of mosquitoes in a focus of dirofilariasis in north-western Iran, with the first finding of filarial larvae in naturally infected lo¬cal mosquitoes. Med Vet Entomol. 23 (2): 111–121.
Wirth MC, Georghiou GP (1996) Organo-phosphate resistance in Culex pipiens from Cyprus. J Am Mosq Control As-soc. 2(1): 112–118.
Chandre F, Darriet F, Doannio JM, Rivi-Ère F, Pasteur N, Guillet P (1997) Distribution of organophosphate and carbamate resistance in Culex pipiens quinquefasciatus (Diptera: Culicidae) in West Africa. J Med Entomol. 34 (6): 664–671.
Nazni WA, Lee HL, Azahari AH (2005) Adult and larval insecticide suscepti-bility status of Culex quinquefasciatus (Say) mosquitoes in Kuala Lumpur, Malaysia. Trop Biomed. 22(1): 63–68.
Cui F, Raymond M, Qiao CL (2006) In-secticide resistance in vector mosqui-toes in China. Pest Manag Sci. 62 (11): 1013–1022.
Kumar K, Sharma AK, Kumar S, Patel S, Sarkar M, Chauhan LS (2011) Multi¬ple insecticide re¬sistance/ susceptibility status of Culex quinquefasciatus, prin¬cipal vector of bancroftian filariasis from filaria en¬demic areas of northern India. Asian Pac J Trop Med. 4(6): 426–429.
Morteza Z, Mousavi SB, Baghestani MA, Aitio A (2017) An assessment of ag-ricultural pesticide use in Iran, 2012–2014. J Environ Health Sci Eng. 15 (1): 10.
Azari-Hamidian S, Harbach RE (2009) Keys to the adult females and fourth-instar larvae of the mosquitoes of Iran (Diptera: Culicidae). Zootaxa. 2078: 1–33.
WHO (2016) Test procedures for insecti-cide resistance monitoring in malaria vector mosquitoes. 2nd ed. World Health Organization, Geneva, p. 55.
WHO (2014) Pesticide Evaluation Scheme. Discriminating concentra¬tions of insec¬ticide for adult mosqui¬toes. World Health Organization, Ge¬neva. Avail¬able at: http://who.int/whopes/resistance/en/Diagnostic_concentrations_May_2014.pdf.
WHO (2005) Guidelines for laboratory and field testing of mosquito larvi¬cides. WHO/CDS/WHOPES/GCDPP/2005.13.
Bisset J, Rodriguez M, Soca A, Pasteur N, Raymond M (1997) Cross-re¬sistance to pyrethroid and organo¬phosphorus insecticides in the south¬ern house mos¬quito (Diptera: Cu¬licidae) from Cuba. J Med Entomol. 34(2): 244–246.
Kolaczinski JH, Curtis CF (2004) Inves-tigation of negative cross-resistance as a resistance-management tool for insecticide-treated nets. J Med Ento-mol. 41(5): 930–934.
Fathian M, Vatandoost H, Moosa-KazemiSH, Raeisi A, Yaghoobi-Ershadi MR, Oshaghi MA, Sedaghat MM (2015) Susceptibility of Culicidae mosqui-toes to some insecticides recommend¬ed by WHO in a malaria endemic area of southeastern Iran. J Arthropod-Borne Dis. 9(1): 22–34.
Nazari M, Janbakhsh B (2000) A survey of the susceptibility level of Culex theileri and Cx. pipiens to DDT, Dieldrin, Propoxur and Malathion in the southern area of Tehran. J Urmia Univ Med Sci. 11(1): 13–19.
Ataie A, Moosa-Kazemi SH, Vatandoost H, Yaghoobi-Ershadi MR, Bakhshi H, Anjomruz M (2015) Assessing the sus¬ceptibility status of mosquitoes (Dip¬tera: Culicidae) in a Dirofilariasis focus, Northwestern Iran. J Arthro¬pod-Borne Dis. 9(1): 7–21.
Naseri-Karimi N, Vatandoost H, Bagheri M, Chavshin AR (2015) Susceptibil-ity status of Culex pipiens against del¬tamethrin and DDT, Urmia County, West Azerbaijan Province, north¬west¬ern Iran. Asian Pac J Trop Dis. 5 (Suppl 1): S77–S79.
Salim-Abadi Y, Oshaghi MA, Enayati AA, Abai MR, Vatandoost H, Eshraghian MR, Mirhendi H, Hanafi-Bojd AA, Gorouhi MA, Rafi F (2016) High in-secticides resistance in Culex pipiens (Diptera: Culicidae) from Tehran, Cap¬ital of Iran. J Arthropod Borne Dis. 10 (4): 483–492.
Vatandoost H, Ezeddinloo L, Mahvi AH, Abai MR, Kia EB, Mobedi I (2004) Enhanced tolerance of house mosqui-to to different insecticides due to ag-ricultural and household pesticides in sewage system of Tehran, Iran. Irani-an J Environ Health Sci Eng. 1(1):42–45.
Al-Sarar AS, Al-Shahrani D, Bayoumi AE, Abobakr Y, Hussein HI (2011) Labor¬atory and field evaluation of some chem¬ical and biological larvi-cides against Culex spp. (Diptera: Cu-licidae) immature stages. Int J Agric Biol. 13: 115–119.
Abai MR, Hanafi-Bojd AA, Vatandoost H (2016) Laboratory evaluation of Temeph¬os against Anopheles stephen¬si and Culex pipiens larvae in Iran. J Arthropod-Borne Dis. 10(4): 510–518.
Cui F, Lin LF, Qiao CL, Xu Y, Marquine M, Weill M, Raymond M (2008) In-secticide resistance in Chinese popu-lations of the Culex pipiens complex through esterase overproduction. En¬to¬mol Experiment Appl. 120(3): 211–220.
|Issue||Vol 12 No 1 (2018)|
|Culex pipiens Insecticide resistance Temephos Propoxur Deltamethrin|
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