Background: Indian tick typhus (ITT) is a type of spotted fever caused by intracellular Rickettsial pathogens, specifi­cally Rickettsia conorii subspecies indica, which exhibits unique clinical symptoms in humans compared to other spot­ted fevers. Human cases have been reported from several states based on hospital-based studies. However, research on the detection of these pathogens in ticks is limited.
Methods: A tick survey was conducted in different agro-climatic regions of Tamil Nadu. A total of 3,016 ticks belong­ing to 12 species in 532 pools were tested for the presence of the groEL gene of Rickettsia conorii indica using indige­nously designed primers.
Results:  Of the 532 pools tested, 44 pools (8.27 %) were positive for the groEL gene, resulting in an overall Minimum Infection Rate (MIR) of 1.50 (95% CI=1.11–1.98). The bacteria were identified in all agro-climatic regions except in the Cauvery delta region. The MIR of the screened tick species were 4.11 (95% CI=1.73–8.43) in Rhipicephalus mi­croplus, 1.34 (95% CI=0.90–1.92) in Haemaphysalis bispinosa, and 2.85 (95% CI=1.56–4.83) in Haemaphysalis inter­media.  The MIR of the ticks collected from cattle was 0.34 in cattle (95% CI=0.15–0.67), 5.50 (95% CI=3.97–7.44) in goats, and 1.37 (95% CI=0.36–3.71) in sheep.
Conclusion: The present study reports that the groEL gene as preliminary marker for the detection of the ITT-causing pathogen in field-collected ticks. The positivity of the groEL gene in the salivary glands needs further experimental con­firmation. The primer needs further evaluation by comparing with MLST (Multilocus sequence typing) sequencing.

Background: Aedes aegypti is a major vector of numerous arboviruses, including dengue, Zika, chikungunya and yel­low fever. This study aimed to establish a standardized and practical procedure for maintaining and propagating an Ae. aegypti colony under controlled insectary conditions in Bandar Abbas, Iran.
Methods: Environmental parameters were routinely monitored. Larvae were reared with an appropriate diet under a regular feeding schedule, while adult mosquitoes were provided with a sucrose solution and blood meals. The correla­tion between the duration of egg paper drying and the hatching percentage was evaluated. Hatching rates were com­pared across three media: cooled boiled water, yeast and larval food.
Results: A strong negative correlation was observed between drying duration and hatching percentage, with a Pearson correlation of −0.818 (p< 0.001). Gradual drying of egg papers over a period of two days resulted in a hatching rate of over 90% without any hatching stimulant. No significant differences in hatching rates were found among the three me­dia, as indicated by F= 0.601 (p= 0.578). In a novel observation, female mosquitoes were found to deposit ap­proxi­mately 20−30% of their eggs directly on the water surface inside ovitraps under insectary conditions in Iran.
Conclusion: This study provides a reliable, low‑cost model for rearing Ae. aegypti in research insectaries and facilitates future studies on vector control.

Background: Aedes albopictus, a key vector of arboviral diseases, has already been detected in Guilan Province, Iran, and is emerging as a public health concern in the country. This study aimed to identify monthly high-risk areas of Ae. albopictus and predict its distribution in Guilan Province using ecological niche modeling under current and projected climate scenarios. Guilan Province, with its high annual influx of travellers and active maritime ports, represents a stra­tegic area for monitoring Ae. albopictus establishment.
Methods: A total of 200 occurrence points were obtained from local entomological surveys and health authorities. Four MaxEnt models were developed: current baseline (bioclimatic variables + elevation); current enhanced (bioclimatic variables + elevation + LST, NDVI, NDWI and population); and two future scenarios (SSP1-2.6 and SSP5-8.5, year 2030, CMIP6). Model performance was excellent, with AUC values of 0.909 and 0.908 for the baseline and enhanced models, respectively. Monthly thermal suitability maps were generated based on 2024 meteorological data using the optimal temperature range of 15–35 °C.
Results: Under the SSP5-8.5 scenario, risk areas expanded, and the monthly analysis indicated that nearly the entire Guilan Province exhibited suitable conditions for seven months of the year. The warm months showed the highest prob­ability of Ae. albopictus presence across the province.
Conclusions: These findings provide critical insights for targeted vector surveillance and control strategies in the re­gion.

Background: Dengue remains an important public health problem in tropical and subtropical settings. Aedes aegypti is a principal dengue vector and is highly adapted to domestic environments. Because repeated chemical-based control may contribute to insecticide resistance and environmental exposure, practical non-insecticide tools are needed. This study evaluated a non-insecticide multimodal trap integrating carbon dioxide, lactic acid, ultraviolet-A light and sound frequency for Ae. aegypti control.
Methods: This quasi-experimental study evaluated a laboratory prototype multimodal trap. A total of 2,850 female Ae. aegypti mosquitoes were used for multimodal combination testing and commercial-trap comparison after preliminary attractant optimization. The colony was obtained from the Parasitology Laboratory, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia. Non-blood-fed females aged 3–5 days were used. Each treatment included three replications of 50 mosquitoes. Outcomes were analyzed using the General Linear Model and repeated ANOVA.
Results: Lactic acid was the most effective single attractant (78.66%). The four-mode combination produced the highest mean effectiveness (91.33%), with a maximum observed value of 98%. In the enclosed-room test, the multimodal trap performed significantly better than the Mosquito Killer BG-360 and the suction-only negative control, but not sig­nifi­cantly better than the Photocatalyst Mosquito and Fly Trap.
Conclusion: The non-insecticide multimodal trap showed promising laboratory effectiveness for capturing or elec­tro­cuting Ae. aegypti. Semi-field and field trials are needed to assess operational effectiveness, feasibility, electrical safety and durability.

Background: The moth fly Clogmia albipunctata (Diptera: Psychodidae) has received little attention as a potential me­chanical vector of drug-resistant pathogens in hospital settings. This study aimed to evaluate the antifungal susceptibil­ity profile of fungal isolates recovered from moth flies collected in teaching hospitals of Babol, Iran.
Methods: Antifungal susceptibility profile evaluation was performed on 50 fungal isolates (including 36 filamentous fungi and 14 yeasts) collected from moth flies in teaching hospitals of Babol city, using the broth microdilution method in accordance with the guidelines of the Clinical and Laboratory Standards Institute (CLSI). The concentrations of anti­fungal drugs ranged from 0.032 to 64 μg/mL. Geometric mean (G-mean), MIC_50, and MIC₉₀ values ​were calculated for statistical analysis.

Results: Luliconazole and posaconazole exhibited the lowest MIC values against most Aspergillus isolates. Similarly, posaconazole and voriconazole displayed potent antifungal activity against Candida albicans isolates, while up to 90% of C. albicans exhibited high-level fluconazole resistance (MIC ≥ 8 µg/mL).
Conclusion: Hospital-associated moth flies were found to carry pathogenic fungi with unusual antifungal resistance patterns. These findings highlight that such insects may serve as potential mechanical carriers of drug-resistant fungi. Nonetheless, monitoring synanthropic insects could be important as a complementary measure in hospital infection con­trol programs.

Background: The mosquito Aedes aegypti is the most important vector of many viral diseases, such as dengue virus. Thus, various chemical insecticides are being used to control this vector. On the other hand, due to the intensive blind use of chemical insecticides, mosquito vectors are rapidly developing resistance against most of them.
Methods: The present study was conducted during 2024–25 to explore the development of resistance in three Ae. ae­gypti strains with a previous history of insecticidal records compared with Laboratory (LAB) and Tidiwala (TDW) strains collected from localities (Tidiwala and rural barren land) with no or minimal use of insecticides. Resistance de­velopment was recorded by utilizing five commonly used insecticides against mosquitoes in Punjab, Pakistan.
Results: Results showed that deltamethrin was the best during 2024 when compared with alpha-cypermethrin, temeph­os, lambda-cyhalothrin and Bacillus thuringiensis israelensis. All field-collected strains revealed significant resistance to the tested insecticides compared with LAB and TDW strains. The same findings were reflected in the enzyme spec­trum (glutathione-S-transferase, mixed-function oxidases, esterase, and acetylcholinesterase).
Conclusion: These results may help to devise a comprehensive future strategy for mosquito control through selective insecticides by limiting the spread of resistance.

Vector-borne diseases remain among the most persistent and dynamic threats to glob­al health. According to the World Health Organ­ization (WHO), these diseases account for more than 700,000 deaths an­nually and im­pose a dis­proportionate bur­den on tropical and subtropi­cal populations, particularly in low- and mid­dle-income countries (1). Mos­quitoes, ticks, sand flies, triatomines, black flies and other hema­tophagous arthropods transmit pathogens re­spon­sible for malaria, dengue, lymphatic fila­riasis, leishmaniasis, Chagas disease, yellow fe­ver, Zika, chikungunya, tick-borne encephali­tis and numerous emerging zoon­oses. Rapid urbani­zation, globalization, en­vironmental deg­rada­tion, climate change and insecticide re­sistance are reshaping the epidemiology and geographic distribution of these infections, in­creasing both their incidence and unpredict­ability (2, 3).

We report a historical record of the reptile-associated tick Amblyomma helvolum Koch, 1844 parasitizing the equatorial spitting cobra Naja sumatrana Laurenti, 1768, based on a preserved specimen housed in the herpetolog­ical collection of the Naturhistorisches Muse­um Wien (Natural History Museum Vienna, NMW), Austria.

Globally, vector-borne diseases (VBDs) ac­count for nearly 20% of the estimated bur­den of infectious diseases. Within the 22 countries of the World Health Organization Eastern Med­iterranean Region (EMR), home to approximate­ly 8% of the world’s popula­tion, nearly 11% of the global VBDs burden is concentrated. Some parasitic diseases, es­pecially malaria, tryp­anosomiasis, schistoso­miasis, onchocerciasis, loi­asis, dracunculiasis and both cutaneous and visceral leishmania­sis, continue to be reported in several coun­tries of the region (1). Further­more, recent regional evidence highlights a substantial di­versity of at least 73 arthropod-borne and ar­thropod-related viruses circulat­ing across EMR countries, notably dengue, West Nile, Crimean-Congo hemorrhagic fever, Rift Val­ley fever, Japanese encephalitis, yellow fe­ver, sandfly fever and chikungunya (2).