Antiplasmodial Property of Glycyrrhiza glabra Traditionally Used for Malaria in Iran: Promising Activity with High Selectivity Index for Malaria

  • Ali Ramazani Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
  • Mahdi Tavakolizadeh Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran Department of Pharmacognosy, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
  • Samira Ramazani Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
  • Hamidreza Kheiri-Manjili Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
  • Mehdi Eskandari Department of Physiology and Pharmacology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Keywords: Glycyrrhiza glabra, Malaria, Traditional medicine, Plasmodium berghei, Iran

Abstract

Background: Development of resistance against the frontline anti-malarial drugs has created an alarming situation, which requires intensive drug discovery to develop new, more effective, affordable and accessible anti-malarial agents. The aim of this study was to assess antiplasmodial activity of the different fractions of root extract of Glycyr­rhiza glabra.Methods: Roots of G. glabra were collected from Tarom district of Zanjan Province in 2016 and then dried root ma­terial was chopped and consecutively extracted by the percolation method using solvents of different polarity. Result­ing extracts were assessed for in vitro and in vivo anti-malarial and cell cytotoxicity activities.Results: Among the three different solvent fractions studied, water-methanol and ethyl acetate fractions showed promising in vitro antiplasmodial activity against CQ-sensitive Plasmodium falciparum 3D7 strain (IC50= 9.95 and 13µg/ml, respectively). Further, the selectivity indices (HeLa cells versus P. falciparum) for the promising water-methanol fraction showed selectivity for P. falciparum and potential safer therapy for human. Interestingly, water-methanol and ethyl acetate fractions showed a significant suppression of parasite growth (72.2% and 65%, respec­tively) in comparison with control group in mice infected with P. berghei (P< 0.05).Conclusion: The promising antiplasmodial activity of the aqueous fraction of G. glabra obtained in our study war­rant bioassay-guided fractionation of this fraction to identify active principles responsible for antiplasmodial activity.

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Published
2018-06-13
How to Cite
1.
Ramazani A, Tavakolizadeh M, Ramazani S, Kheiri-Manjili H, Eskandari M. Antiplasmodial Property of Glycyrrhiza glabra Traditionally Used for Malaria in Iran: Promising Activity with High Selectivity Index for Malaria. jad. 12(2):135-40.
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Original Article