Assessment the Changing Trend of Susceptibility to Two Insecticides Among Field-Population Culex quinquefasciatus Compared with the Same Population Undergoing to Multiple Colonization
AbstractBackground: During the past decade, rapid development of insecticide resistance have been reported among many species of mosquito vectors against four main categories of insecticides worldwide. The aim of the research was to assess the variation trend of susceptibility levels of Culex quinquefasciatus to two insecticides separately for the field population compared with subsequent generations of the same sample after multiple colonization.Methods: Larvae and pupae of Cx. quinquefasciatus were collected from house sewages and reared to adult which blood-fed on roosters. Ten percent sucrose fed female mosquitoes aged 2–3 days were used for susceptibility tests with DDT and deltamethrin. Susceptibility levels was assessed in the adult stage of field stran Cx. quinquefasciatus against DDT 4.0% and deltamethrin 0.05% and continued up to next six generations undergoing multiple rearing at insectary condition.Results: The susceptibility levels to DDT 4.0% did not change compared to the field with the lab population to six generations. Regarding deltamethrin 0.05%, no significant difference was shown between field strain (58.3%) and 3rd generation (52.7%) compared to the 6th one (33.8%).Conclusion: This finding may reflect the role of the kdr gene in resistance to organochlorine which has cross-resistance with pyrethroid insecticides. The results of this study clearly showed the irreversible trend of pyrethroid resistance among colonized mosquitoes. This is the first study of the resistance status of Cx. quinquefasciatus in Iran.
2. Manimegalai K, Sukanya S (2014) Biolo-gy of the filarial vector, Culex quin-que¬fas¬ciatus (Diptera:Culicidae). Int J Curr Microbiol App Sci. 3(4): 718–724.
3. Njenga SM, Muita M, Kirigi G, Mbugua J, Mitsuiy Y, Fujimai Y, Aoki Y (2000) Bancroftian filariasis in Kwale district, Kenya. East Afr Med J. 77(5): 245–249.
4. Xiao-Xia G, Chun-Xiao L, Yong-Qiang D, Dan X, Qin-Mei L, Qun W, Ai-Juan S, Yan-De D, Wu-Chun C, Cheng-Feng Q, Tong-Yan Z (2016) Culex pipiens quin¬quefasciatus: a po-tential vector to trans¬mit Zika virus. Emerg Microbes Infect. 5(9): 1–5.
5. Norris LC, Norris DE (2011) Insecticide resistance in Culex quinquefasciatus mos¬quitoes after the introduction of in¬secticide-treated bed nets in Macha, Zambia. J Vector Ecol. 36(2): 411–420.
6. Yadouléton A, Badirou K, Agbanrin R, Jöst H, Attolou R, Srinivasan R, Pa-donou G, Akogbéto M (2014) Insecti-cide re¬sistance status in Culex quin-quefascia¬tus in Benin. Parasit Vec-tors. 8(7): 1–6.
7. Jones CM, Machin C, Mohammed K, Majambere S, Ali AS, Khatib BO, Mcha J, Ranson H, Kelly-Hope LA (2012) In-secticide resistance in Culex quinque-fasciatus from Zanzibar: im¬pli¬ca¬tions for vector control programs. Parasit Vectors. 5 (78): 1–9.
8. Chandre F, Arriet F, Darder M, Cuany A, Doannio JMC, Pasteura N, Guillet P (1998) Pyrethroid resistance in Culex quinquefasciatus from West Africa. Med Vet Entomol. 12: 359–366.
9. Corbel V, Guessan RN, Brengues C, Chan¬dre F, Djogbenou L, Martin T, Akogbe¬to M, Hougard JM, Rowland M (2007) Multiple insecticide re-sistance mecha¬nisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Af¬rica. Acta Trop. 101: 207–216.
10. Kudom AA, Mensah BA, Froeschl G, Rinder H, Boakye D (2015) DDT and pyrethroid resistance status and labor-a¬tory evaluation of bio-efficacy of long lasting insecticide treated nets against Culex quinquefasciatus and Culex de¬cens in Ghana. Acta Trop. 150: 122–130.
11. Agrawal A, Pandey RS, Sharma B (2010) water pollution with special reference to pesticide contamination in India. J Wa¬ter Res Prot. 2: 432–448.
12. Priester TM, Georghiou GP (1978) In-duc¬tion of high resistance to perme-thrin in Culex pipiens quinquefascia¬tus. J Econ Entomol. 71(2): 197–200.
13. Peiris H, Hemingway J (1990) Mecha¬nisms of insecticide resistance in a temephos selected Culex quinquefas¬cia¬tus (Dip-tera: Culicidae) strain from Sri Lanka. Bull Entomol Res. 80(4): 453–457.
14. Yanola J, Chamnanya S, Lumjuan N, Som-boon P (2015) Insecticides re¬sistance in the Culex quinquefasciatus popula-tions from northern Thailand and pos-sible resistance mechanisms. Acta Trop. 149: 232–238.
15. Shah RM, Alam M, Ahmad D, Waqas M, Ali Q, Binyamin M, Ali Sarfraz (2016) Toxicity of 25 synthetic insec-ticides to the field population of Culex quinque¬fasciatus say. Parasitol Res. 115 (11): 4345–4351.
16. Amin AM, Peiris HTR (1990) Detection and selection of organophosphate and carbamate resistance in Culex quin-que¬fasciatus from Saudi Arabia. Med Vet Entomol. 4: 269–273.
17. World Health Organization (2016) Test procedures for insecticide resistance mon¬itoring in malaria vector mosqui-toes -2nd ed. WHO Document Produc¬tion Services, Geneva, Switzerland. Avail¬able at: http://apps.who.int/iris/bitstream/10665/250677/1/9789241511575-eng.pdf.
18. World Health Organization (2014) Pes-ticide evaluation scheme. Discrimi-nating concentrations of insec¬ticide for adult mosquitoes. World Health Organization, Geneva: Available at: https://www.who.int/neglected_diseases/vector_ecology/resources/en/Diagnostic_concentrations.pdf?ua=1
19. Sathantriphop S, Paeporn P, Supaphath-om K (2006) Detection of insecticides re¬sistance status in Culex quinquefas-cia¬tus and Aedes aegypti to four major groups of insecticides. Trop Biomed. 23(1): 97–101.
20. Sarkar M, Bhattacharyya IK, Borkotoki A, Goswami D, Rabha B, Baruah I, Sri¬vastava RB (2009) Insecticide re-sistance and detoxifying enzyme ac-tiv¬i¬ty in the principal bancroftian fila-ria¬sis vector, Culex quinquefasciatus, in north¬eastern India. Med Vet Entomol. 23(2): 122–131.
21. Tantelya ML, Pablo Tortosab, Alouta H, Berticata C, Berthomieua A, Ruteec A, Dehecqc JS, Makoundoua P, Labbéa P, Pasteura N, Weilla M (2010) Insec¬ti¬cide resistance in Culex pipiens quinquefas¬ciatus and Aedes albopictus mos¬quitoes from La Réu-nion Island. Insect Bio¬chem Mol Biol. 40(4): 317–324.
22. Brogdon WG, McAllister JC (1998) In-secticide resistance and vector con-trol. Emerg Infect Dis. 4(4): 605–613.
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