Review Article

Monitoring and Mapping of Insecticide Resistance in Medically Important Mosquitoes (Diptera: Culicidae) in Iran (2000–2020): A Review


Background: Mosquitos due to their role in the transmission of different pathogens to humans are considered as an important group in the phylum Arthropoda. According to the WHO and FAO guideline different groups of insecti­cide applied for controlling pests in both the agricultural and public health sectors.Methods: All the data published about resistant status of the mosquitoes Anopheles, Culex, Aedes and Culiseta spe­cies were searched on PubMed, Elsevier, Web of Science, Magiran and google scholar. The objectives of this study was to review the trend of resistance to insecticides during 2000–2020 in medically important mosquitoes in Iran. The criteria for resistant are followed according to WHO guideline.Results: The Results showed that there are widespread, multiple resistances in the country to different organochlo­rine, organophosphates, carbamate and pyrethroids insecticides in the mosquitoes.Conclusion: The effect of pesticide residues on the environment could be a cause for selection pressure on mosqui­tos and lead to insecticides resistance to them. Insecticides resistance is main challenge of the vector control pro­gram. Also result will provide a guideline for control of the mosquito-borne diseases in the country as well as the world.
1. Mullen G, Durden L (2009) Medical and Veterinary Entomology, 2ed edition, Mos¬quitoes (Culicidae) Woodbridge A. Fos¬ter and Edward D Walter. Vol. 2. Else¬vier, Burlington.
2. Service MW (2003) Medical Entomology for Students. Vol. 3. United Kingdom: Cambridge. University Press, Cam-bridge.
3. Vatandoost H, Raeisi A, Saghafipour A, Nikpour F, Nejati J (2019) Malaria situ-ation in Iran: 2002–2017. Malar J. 18(1): 200.
4. Edrissian GH (2006) Malaria past and pre-sent situation in Iran. Iran J Para¬sitol. 1 (1): 1–14.
5. Yousef Mogaddam M, Motevalli-Haghi F, Fazeli-Dinan M, Hosseini-Vasoukolaei N, Enayati AA (2016) A review of in-secticide resistance in malaria vectors of Iran. J Mazandaran Univ Med Sci. 25 (134): 394–411.
6. Vatandoost H, Zahirnia AH (2010) Re-spon¬siveness of Anopheles maculi¬pennis to different imagicides during resurgent malaria. Asian Pacific J Trop Med. 3: 360–363.
7. Naficy K, Saidi S (1970) Serological sur-vey on viral antibodies in Iran. Trop Geogr Med. 2: 183–188.
8. Azari-Hamidian S, Yaghoobi-Ershadi MR, Javadian E, Abai MR, Mobedi I, Linton YM, Harbach RE (2009) Dis¬tribution and ecology of mosquitoes in a focus of dirofilariasis in northwest¬ern Iran, with the first finding of filar¬ial larvae in nat-urally infected local mosquitoes. Med Vet Entomol. 23(2): 111–121.
9. Salim-Abadi Y, Oshaghi MA, Enayati AA, Abai MR, Vatandoost H, Eshraghian MR (2016) High insecti¬cides resistance in Culex pipiens (Diptera: Culicidae) from Tehran, capital of Iran. J Arthro-pod Borne Dis. 10(4): 483–492.
10. Nikookar SH, Fazeli-Dinan M, Ziapour SP, Ghorbani F, Salim-Abadi Y, Vatan-doost H, Enayati AA (2019) First report of biochemical mecha¬nisms of insecti-cide resistance in the field population of Culex pipiens (Diptera: Culicidae) from Sari, Ma¬zandaran, north of Iran. J Ar-thropod Borne Dis. 13(4): 378–390.
11. Lotfi MD, Manouchehri AV, Yazdanpanah H (1975) Resistance of Cx. pipiens pipiens to DDT in northern Iran, 1973. Bull Soc Pathol Exot Fil-iales. 68(1): 91–93.
12. Bagheri M, Terenius O, Oshaghi MA, Motazakker M, Asgari S, Dabiri F, Vatan¬doost H, Mohammadi Bavani M, Chavshin AR (2015) West Nile Virus in mosquitoes of Iranian wetlands. Vector Borne Zoonotic Dis. 15(12): 750–754.
13. Fathian M, Vatandoost H, Moosa-Kazemi SH, Raeisi A (2015) Suscep¬tibility of Cu¬licidae mosquitoes to some insecti-cides recommended by WHO in a ma-laria endemic area of southeastern Iran. J Arthropod Borne Dis. 9(1): 22–34.
14. Ataie A, Moosa-Kazemi SH, Vatan¬doost H, Yaghoobi-Ershadi MR, Bakhshi H, Anjomruz M (2015) As¬sessing the sus-ceptibility status of mosquitoes (Diptera: Culicidae) in a dirofilariasis focus, north¬western Iran. J Arthropod Borne Dis. 9 (1): 7–21.
15. Morteza Z, Mousavi SB, Baghestani MA, Aitio A (2017) An assessment of agri-cultural pesticide use in Iran, 2012–2014. J Environ Health Sci Eng. 15(1): 1–8.
16. Vatandoost H, Ezeddinloo L, Mahvi AH, Abai MR,Kia EB, Mobedi I (2004) En-hanced tolerance of house mosquito to different insecticides due to agricultural and household pesti¬cides in sewage sys-tem of Tehran, Iran. Iran J Environ Health Sci Eng. 1(1): 42–45.
17. World Health Organization (WHO) (1998) Test procedures for insecticide resistance monitoring in malaria vec¬tors, bio-effi¬cacy and persistence of insecticides on treated surfaces: report of the WHO in¬formal consultation, Geneva, 28–30 Sep¬tember 1998. p. 46.
18. World Health Organization (WHO) (2016) Test procedures for insecticide resistance monitoring in malaria vec¬tor mosquitoes. 2nd edition. p. 56.
19. Gorouhi MA, Oshaghi MA, Vatandoost H, Enayati AA, Raeisi A, Abai MR (2018) Biochemical basis of cyfluthrin and DDT resistance in Anopheles ste-phensi (Diptera: Culicidae) in malari¬ous area of Iran. J Arthropod Borne Dis. 12 (3): 310–320.
20. Zahirnia A, Vatandoost H, Nateghpour M, Djavadian E (2002) Insecticide re-sistance/ susceptibility monitoring in Anoph¬eles pulcherrimus (Diptera: Cu-licidae) in Ghasreghand district, Sistan and Baluchistan Province, Iran. Iran J Public Health. 31(1–2): 11–14.
21. Lak SS, Vatandoost H, Entezarmahdi M, Ashraf H, Abai M, Nazari M (2002) Mon¬itoring of insecticide re¬sistance in Anopheles sacharovi (Fa¬vre, 1903) in bor¬derline of Iran, Ar¬menia, Naxcivan and Turkey, 2001. Iran J Public Health. 31 (3–4): 96–99.
22. Borhani N, Vatandoost H (2004) Suscep-tibility and irritability levels of main malaria vectors to synthetic py¬rethroids in the endemic areas of Iran. Acta Med Iran. 42(4): 240–247.
23. Vatandoost H, Abdoljabari Boonab R, Abai MR, Oshaghi MA (2005) Entomo-logical survey in Kali¬bar, a resurgent malaria focus in East-Azerbaijan, Iran.
Pak J Biol Sci. 8(10): 1466–1471.
24. Vatandoost H, Mashayekhi M, Abai MR, Aflatoonian M, Hanafi-Bojd AA, Shar-ifi I (2005) Monitoring of insecti¬cides re¬sistance in main malaria vec¬tors in a malarious area of Kahnooj District, Ker-man Province, southeast¬ern Iran. J Vec-tor Borne Dis. 42(3): 100–108.
25. Vatandoost H, Oshaghi MA, Abai MR, Shahi M, Yaaghoobi F, Baghaii M, Hanafi-Bojd AA, Zamani G, Townson H (2006) Bionomics of Anopheles ste-phensi Liston in the malarious area of Hormozgan Province, Southern Iran, 2002. Acta Trop. 97(2): 196–203.
26. Davari B, Vatandoost H, Ladonni H, Shaeghi M, Oshaghi MA, Basseri HR, Enayati AA, Rassi Y, Abai MR, Hanafi-Bojd AA, Akbarzadeh K (2006) Com-par¬ative efficacy of differ¬ent imagicides against different strains of Anopheles stephensi in the malaroius areas of Iran, 2004–2005. Pakistan J Biol Sci. 9(5): 885–892.
27. Abai MR, Mehravaran A, Vatandoost H, Oshaghi MA, Javadian E, Mashay¬ekhi M, Mosleminia A, Piyazak N, Edallat H, Mohtarami F, Jabbari H, Rafi F (2008) Comparative perfor¬mance of imagicides on Anopheles stephensi, main malaria vec¬tor in a malarious area, southern Iran. J Vector Borne Dis. 45(4): 307–312.
28. Hanafi-Bojd AA, Vatandoost H, Oshaghi MA, Haghdoost AA, Shahi M, Sedaghat MM, Abedi F, Yeryan M, Pakari A (2012) Entomological and epidemio¬log¬ical attributes for malaria transmission and implementa¬tion of vector control in southern Iran. Acta Trop. 121(2): 85–92.
29. Soleimani-Ahmadi M, Vatandoost H, Shayeghi M, Raeisi A, Abedi F, Eshraghian MR, Madani A, Safari R, Shahi M, Mojahedi A, Poorahmad-Gar-bandi F (2012) Vector ecology and sus-ceptibility in a malaria endemic fo¬cus in southern Islamic Republic of Iran. East
Mediterr Health J. 18: 1034–1041.
30. Vatandoost H, Abai MR (2012) Irritabil-ity of malaria vec¬tor, Anopheles sacha-rovi to different insecticides in a ma¬lar-ia–prone area. Asian Pac J Trop Med. 5 (2): 113–116.
31. Vatandoost H, Hanafi-Bojd AA (2012) Indication of pyrethroid resistance in the main malaria vector, Anopheles ste-phensi from Iran. Asian Pac J Trop Med. 5(9): 722–726.
32. Nejati J, Vatandoost H, Oshaghi MA, Salehi M, Mozafari E, Moosa-Kazemi SH (2013) Some ecological attributes of malarial vector Anopheles super¬pictus Grassi in endemic foci in Southeastern Iran. Asian Pac J Trop Biomed. 3(12): 1003–1008.
33. Chavshin AR, Dabiri F, Vatandoost H, Bavani MM (2015) Susceptibility of Anoph¬eles maculipennis to different clas¬ses of insecticides in West Azarbaijan Province, Northwestern Iran. Asian Pac J Trop Biomed. 5(5): 403– 406.
34. Gorouhi MA, Vatandoost H, Oshaghi MA, Raeisi A, Enayati AA, Mirhendi H (2016) Current susceptibility status of Anoph¬eles stephensi (Diptera: Cu¬licidae) to dif¬ferent imagicides in a malarious area, southeastern of Iran. J Arthropod Borne Dis. 10(4): 493–500.
35. Zare M, Soleimani-Ahmadi M, Davoodi SH, Sanei-Dehkordi A (2016) Insecti-cide sus-ceptibility of Anopheles ste-phensi to DDT and current insecticides in an elim¬ination area in Iran. Parasit Vectors. 9 (1): 571–575.
36. Vatandoost H, Hanafi-Bojd AA, Raeisi A, Abai MR, Nikpour F (2017) Ecol¬o-gy, monitoring and map¬ping of insecti-cide resistance of ma¬laria vector, Anoph-eles culicifa¬cies (Diptera: Culicidae) to different imagicides in Iran. Asian Pac J Trop Dis. 7(1): 53–56.
37. Vatandoost H, Abai MR, Akbari M, Raeisi A, Yousefi H, Sheikhi S, Bagheri A (2019) Comparison of CDC bottle bio¬assay with WHO standard method for assessment susceptibility level of ma¬laria vector, Anopheles ste¬phensi to three imagicides. J Arthro¬pod Borne Dis. 13 (1): 17–26.
38. Nazari M, Janbakhsh B (2000) A survey of the susceptibility level of Culex theil-eri and Cx. pipiens to DDT, Diel¬drin, Propoxur and Malathion in the southern area of Tehran. J Uromia Univ Med Sci. 11(1): 13–19.
39. Naseri-Karimi N, Vatandoost H, Bagheri M, Chavshin AR (2015) Sus¬ceptibility status of Culex pipiens against del-tame-thrin and DDT, Urmia County, West Azer¬baijan Province, northwest-ern Iran. Asian Pac J Trop Dis. 5(Suppl 1): S77–S79.
40. Salim-Abadi Y, Asadpour M, Sharifi I, Sanei-Dehkordi A, Gorouhi MA, Paksa A (2017) Baseline susceptibility of fi-larial vector Culex quinquefasci¬atus (Dip¬tera: Culicidae) to five insec¬ticides with different modes of action in south-east of Iran. J Arthropod Borne Dis. 11(4): 453–462.
41. Ghorbani F, Vatandoost H, Hanafi-Bojd AA, Abai MR, Nikoobar H, Enayati AA (2018) High resistance of vector of West Nile Virus, Culex pipiens Linnaeus (Dip¬tera: Culicidae) to different insecticides recommended by WHO in northern Iran. J Arthropod Borne Dis. 12(1): 24–30.
42. Shemshadian A, Abai MR, Vatandoost H, Dinparast-Djadid N, Oshaghi MA, Mo¬jahedi A (2020) Assessment the chang¬ing trend of susceptibility to two insec¬ticides mmong field-population Culex quin¬quefasciatus compared with the same population undergoing to multiple colo¬nization. J Arthropod Borne Dis. 14(2): 185–192.
43. Zeidabadinezhad R, Vatandoost H, Abai MR, Dinparast-Djadid N, Raz A, Sed¬a-ghat MM, Oshaghi MA, Raeisi A, Adi¬bi N (2019) Target site insensitiv¬ity de¬tec-tion in deltamethrin resistant Culex pipiens complex in Iran. Iran J Public Health. 48(6): 1091–1098.
44. Hassandoust S, Moosa-Kazemi SH, Vatan¬doost H, Sedaghat MM, Akbar-zadeh K (2020) Evaluation of suscep-tibility of Ae¬des caspius (Diptera: Cu-licidae) to in¬sec¬ticides in a potent ar-boviral-prone ar¬ea, southern Iran. J Arthropod Borne Dis. 14(2): 214–227.
45. Hazratian T, Paksa A, Sedaghat MM, Vatan¬doost H, Moosa-Kazemi SH, Sanei-Dehkordi A, Salim-Abadi Y, Pirmoham¬madi M, Yousefi S, Amin M, Oshaghi MA (2019) Baseline Sus-ceptibility of Culiseta longiareolata (Diptera: Cu¬licidae) to Different Ima-gicides, in Eastern Azer¬baijan, Iran. J Arthropod Borne Dis. 13 (4): 407–415.
46. Safi NH, Ahmadi AA, Nahzat S, Warusa-vithana S, Safi N, Valadan R, Shemshad¬ian A, Sharifi M, Enayati A, Heming¬way J (2019) Status of insecti-cide re¬sistance and its biochemical and molec¬ular mechanisms in Anopheles stephensi (Diptera: Culicidae) from Afghanistan. Malar J. 18(1): 249.
47. Yared S, Gebressielasie A, Damodaran L, Bonnell V, Lopez K, Janies D, Carter TE (2020) Insecticide re¬sistance in Anoph¬eles stephensi in Somali Region, eastern Ethiopia. Ma¬lar J. 19: 1–7.
48. Ben Cheikh II, Ben Ali-Haouas Z, Mar- quine M, Pasteur N (1998) Resistance to Organophosphorus and Pyrethroid Insec¬ticides in Culex pipiens (Diptera: Cu¬licidae) from Tunisia. J Med Entomol. 35(3): 251–260.
49. Corbel V, N'Guessan R, Brengues C, Chan¬dre F, Djogbenou L, Martin T, Akogbéto M, Hougard JM, Rowland M (2007) Multiple insecticide re¬sistance mecha¬nisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Af¬rica. Acta Trop. 101: 207–216.
50. Bisset JA, Rodriguez MM, Diaz C, Ortiz E, Marquetti MC, Hemingway J (1990) The mechanisms of organo¬phosphate and carbamate resistance in Culex quinque¬fasciatus (Diptera: Cu¬licidae) from Cuba. Bull Entomol Res. 80(3): 245–250.
51. Salim Abadi Y, Vatandoost H, Rassi Y, Abaei MR, Sanei Dehkordi AR, Paksa A (2010) Evaluation of biological con-trol agents for mosquitoes control in ar-tificial breeding places. Asian Pac J Trop Med. 3(4): 276–277.
52. Vatandoost H, Nikpour F, Hanafi-Bojd AA, Abai MR, Khanavi M, Haji¬iakhon-di A, Raesi A, Nejati J (2019) Efficacy of extractions of Iranian na¬tive plants against main malaria vec¬tor, Anopheles stephensi in Iran for making appropriate formulation for disease control. J Ar-thropod Borne Dis. 13(4): 344–352.
53. Isman MB (2004) Plant essential oils for pest and disease management. Crop Prot. 19(8–10): 603–608.
54. Sedaghat MM, Sanei-Dehkordi AR, Kha-navi M, Abai MR, Mohtarami F, Vatan-doost H (2011) Chemical com¬position and larvicidal activity of es¬sential oil of Cupressus arizonica E.L. Greene against malaria vector Anoph¬eles stephensi Lis-ton (Diptera: Cu¬licidae). Pharmacogn Res. 3(2): 135–139.
55. Sedaghat MM, Sanei-Dehkordi AR, Abai M, Khanavi M, Mohtarami F, Abadi YS, Rafi F, Vatandoost H (2011) Larvicidal activity of essential oils of apiaceae plants against malaria vector, Anopheles ste¬phensi. Iran J Arthropod Borne Dis. 5(2): 51–59.
56. Vatandoost H, Sanei-Dehkordi A, Sadeghi S, Davari B, Karimian F, Abai M (2012) Identification of chemical constituents and larvicidal activity of Kelussia odo¬ratissima Mozaffarian essential oil against two mosquito vectors Anopheles stephen¬si and Culex pipiens (Diptera: Cu¬licidae). Exp Parasitol. 132(4): 470–474.
57. Sanei-Dehkordi A, Soleimani-Ahmadi M, Akbarzadeh K, Salim Abadi Y, Paksa A, Gorouhi MA (2016) Chemi¬cal com-position and mosquito larvi¬cidal prop-erties of essential oil from leaves of an Iranian indigenous plant Zhumeria maj-dae. J Essent Oil Bear Pl. 19(6): 1454–1461.
58. Soleimani-Ahmadi M, Sanei-Dehkordi AR, Turki H, Madani A, Abadi YS, Paksa A (2017) Phytochemical prop-erties and in¬secticidal potential of vol-atile oils from Tanacetum persicum and Achillea kella¬lensis against two medically im¬portant mosquitoes. J Es-sent Oil Bear Pl. 20(5): 1254–1265.
59. Soleimani-Ahmadi M, Abtahi SM, Madani A, Paksa A, Abadi YS, Gor¬ouhi MA (2017) Phytochemical pro¬file and mos¬quito larvicidal activity of the es-sential oil from aerial parts of Satureja bacht¬iarica Bunge against malaria and lym¬phatic filariasis vec¬tors. J Essent Oil Bear Pl. 20 (2): 328–336.
60. Soleimani-Ahmadi M, Gorouhi MA, Mo-hammadi Azani S, Salim-Abadi Y, Paksa A, Rashid G (2017) Larvicidial effects of essential oil and methanol extract of Achillea wilhelmsii C. Koch (Aster¬ace¬ae) against Anopheles ste-phensi Liston (Diptera: Culicidae), a malaria vector. J Kerman Univ of Med Sci. 24(1): 58–67.
61. Sanei-Dehkordi A, Soleimani-Ahmadi M, Salim-Abadi Y, Paksa A (2019) Wild chive oil is an extremely effec¬tive larvi-cide against malaria mosquito vector Anopheles stephensi. Asian Pac J Trop Med. 12(4): 170–174.
62. Karami M, Moosa-Kazemi SH, Oshaghi MA, Vatandoost H, Sedaghat MM, Ra-jabnia R, Hosseini M, Maleki-Rav¬asan N, Yahyapour Y, Ferdosi-Sha¬handashti E (2016) Wolbachia Endo¬bacteria in natural populations of Cu¬lex pipiens of Iran and its phylogenetic congruence. J Ar¬thropod Borne Dis. 10(3): 347–363.
63. Yen PS, Failloux AB (2020) A Review: Wolbachia-based population replace-ment for mosquito control shares com-mon points with genetically modi¬fied con¬trol Approaches. Pathogens. 9(5): 404.
IssueVol 15 No 1 (2021) QRcode
SectionReview Article
Resistance; Insecticide; Mosquito; Iran

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Salim Abadi Y, Sanei-Dehkordi A, Paksa A, Gorouhi MA, Vatandoost H. Monitoring and Mapping of Insecticide Resistance in Medically Important Mosquitoes (Diptera: Culicidae) in Iran (2000–2020): A Review. J Arthropod Borne Dis. 15(1):21–40.