Original Article

Seroepidemiologic Survey of Canine Visceral Leishmaniasis in Tehran and Alborz Provinces of Iran


Background: A two year seroepidemiological study was designed to find out the seroprevalence and risk factors ofcanine visceral leishmaniasis (CVL) among stray and owned dogs in Tehran and Alborz Provinces of Iran.
Methods: Blood samples of  602 dogs living in  11  counties of  Tehran and  Alborz  Provinces were  taken by venipuncture in 2008–2010. After separation of blood sera, anti-leishmanial antibodies were detected by direct agglutination test (DAT).
Results: Overall, of the 408 and 194 serum samples collected randomly from dogs in 11 localities in Tehran and Alborz Provinces, 18/408 (4.41%) and 12/194 (6.18%) respectively were found positive. Among the localities, Shemiran in Tehran Province and Karaj In Alborz Province had the highest prevalence rates. No statistically significant differences were found between sex and living place but there was significant difference between living status (owned or stray) and CVL infection of dogs in Alborz Province (P= 0.018). The highest seroprevalence (7.5%) was found in dogs aged 3 to 5 years old. Only 20% of the seropositive dogs were symptomatic.
Conclusion: Concerning possible human infections in Tehran and Alborz Provinces, both symptomatic and asymptomatic seropositive dogs should be considered as a risk.

Bokai S, Mobedi I, Edrissian GhH, Nadim A (1998) Seroepidemiological study of ca- nine visceral leishmaniasis in Meshkin- Shahr, northwest of Iran. Arch Razi Inst.41–46, 48–49.

Clarisa B, Palatnik-de-Sousa (2008) Vaccines for leishmaniasis in the fore coming 25 years. Vaccine. 26: 1709–1724.

Dantas-Torres F, Edileuza Felinto de Brito M, Pinto Brandao-Filho S (2006) Seroepidemiological survey on canine leishmaniasis among dogs from an ur- ban area of Brazil, Vet Parasitol. 140:54–60.

Fakhar M, Motazedian MH, Asgari Gh, Mo- hebali M, Mehrbani D (2006) Intro- duction of a new endemic foci of kala- azar in south of Iran. Armaghan Danesh J. 11(2): 103–113.

Farzam M, Changizi E, Mohebali M, A- khoundi B, Salimi Bajestani MR (2007) Seroepidemilogical survey of visceral leishmaniasis in shepherd dogs of Jahrom county, Fars Province. Vet J Iran. 4(3): 58–67.

França-Silva J, T da Costa R, Siqueira A, LL Machado-Coelho G, A da Costa C,Mayrink W, P Vieira E, S Costa J, Genaro O, Nascimento E (2003) Epi- demiology of canine visceral leishma- niosis in the endemic area of Montes Claros Municipality, Minas Gerais State, Brazil. Vet Parasitol. 111: 161–173.

Greene Craig E (2006) Infectious diseases of the dog and cat, third edition, WB Saunders Co, Chapter 73, pp: 685–698.

Habibzadeh S, Arshi S, Sadeghi-Bazargani H, Mohebali M (2007) Rk39 Dipstick Test as a practical solution for canine leishmaniasis diagnosis among nomad- ic populations. J Anim Vet Adv. 6(2):175–178.

Harith A, Salappendel RJ, Reiter I, Knapen F, Korte P, Huigen E, Kolk RHG (1989) Application of a direct agglu- tination test for detection of specific anti-Leishmania antibodies in the ca- nine reservoir. J Clin Microbiol. 27:2252–2257.

Jaffe ChL, Baneth G, Abdeen ZA, Schlein Y, Warburg A (2004) Leishmaniasis in Israel and the Palestinian Authority. Trends Parasitol. 20(7): 328–332.

Khanmohammadi M, Fallah E, Rahbari S, Sohrabi I, Farshchian M, Hamzavi F, Mohammadpour Asl A (2010a) Seroepidemiological survey of canine Visceral leishemanisis in Sarab Dis- trict, East Azerbaijan Province, North- west of Iran. Afr J Microbiol Res.4(19): 2022–2028.

Khanmohammadi M, Fallah E, Rahbari S, Sohrabi I, Farshchian M, Hamzavi F, Mohammadpour Asl A (2010b) Study on seroprevalence of canine visceral leishmaniasis (CVL) in ownership dogs of Sarab, East Azerbaijan Province, Northwest of Iran with indirect immuno fluorescence antibody test (IFAT) and its health importance. J Anim Vet Adv.9(1): 139–143.

Mohebali M, Hamzavi Y, Fallah E, Zarei Z (2001) Study of canine visceral leish- maniasis in some parts of Iran and its health importance. J Vet Res. 56: 55–59.

Mohebali M, Khamesipour A, Mobedi I, Zarei Z, Hashemi- Fesharki R (2004) Double blind randomized efficacy trial of alum precipitated autoclaved Leishmania ma- jor vaccine mixed with BCG against canine visceral leishmaniasis in Mesh- kin-shahr district I.R. of Iran. Vaccine.22: 4097–4100.

Mohebali M, Hajjaran H, Hazavi Y, Mobedi I, Arshi Sh, Zarei Z, Akhoundi B, Manouchehri Naeini K, Avizeh R, Fakhar M (2005) Epidemiological as- pects of canine visceral leishmaniosis in the Islamic Republic of Iran. Vet Parasitol. 129: 243–251.

Mohebali M, Edrissian GhH, Shirzadi MR, Akhoundi B, Hajjaran H, Zarei Z, Molaei S, Sharifi I, Mamishi S, Mahmoud-vand H, Torabi V, Moshfe A, Malmasi A, Motazedian MH, Fakhar M (2011) An observational study on the current dis- tribution of visceral leishmaniasis in different geographical zones of Iran and implication to health policy. Travel Med Infect Dis. 9(2): 67–74.

Moreno J, Alvar J (2002) Canine leishma- niasis: epidemiological risk and the ex-perimental model. Trends Parasitol. 18:399–405.

Moshfe A, Mohebali M, Edrissian Gh, Zarei Z, Akhoundi B, Kazemi B, Jamshidi Sh, Mahmoodi M (2008) Seroepidemiological study on canine visceral leishmaniasis in Meshkin-Shahr district, Ardabil Prov- ince, Northwest of Iran. Iran J Para- sitol. 3(3): 1–10.

Moshfe A, Mohebali M, Edrissian Gh, Zarei Z, Akhoundi B, Kazemi B, Jamshidi Sh, Mahmoodi M (2009) Canine visceral leishmaniasis: Asymptomatic infected dogs as a source of L. infantum infec- tion. Acta Trop. 112: 101–105.

Ozensoy S, Ozbel Y, Turgay N, Alkan MZ, Gul K, Gilman-Sachs A, Chang KP, Reed SG, Ali Ozcel M (1998) Serodiagnosis and epidemiology of visceral leishmaniasis in Turkey. Am J Trop Med Hyg. 59(3): 363–369.

Rab MA, Frame IA, Evans DA (1995) The role of dogs in the epidemiology of human visceral leishmaniasis in north- ern Pakistan. T Roy Soc Trop Med H.89: 612–615.

Zaffaroni E, Rubaudo L, Lanfranchi P, Mignone W (1999) Epidemiological patterns of canine leishmaniosis in Western Liguria (Italy). Vet Parasitol.81: 11–19.

IssueVol 8 No 2 (2014) QRcode
SectionOriginal Article
Direct Agglutination Test Dog Iran Visceral leishmaniasis

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How to Cite
Malmasi A, Janitabar S, Mohebali M, Akhoundi B, Maazi N, Aramoon M, Khorrami N, Seifi HA. Seroepidemiologic Survey of Canine Visceral Leishmaniasis in Tehran and Alborz Provinces of Iran. J Arthropod Borne Dis. 1;8(2):132-138.