Assessment of Recombination in the S-segment Genome of Crimean-Congo Hemorrhagic Fever Virus in Iran

  • Sadegh Chinikar Mail Arboviruses and Viral Hemorrhagic Fevers Laboratory (National Reference Lab), Pasteur Institute of Iran, Tehran, Iran.
  • Nariman Shah-Hosseini Arboviruses and Viral Hemorrhagic Fevers Laboratory (National Reference Lab), Pasteur Institute of Iran, Tehran, Iran.
  • Saeid Bouzari Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
  • MohammadAli Shokrgozar National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
  • Ehsan Mostafavi Department of Epidemiology, Pasteur Institute of Iran, Tehran, Iran.
  • Tahmineh Jalali Arboviruses and Viral Hemorrhagic Fevers Laboratory (National Reference Lab), Pasteur Institute of Iran, Tehran, Iran.
  • Sahar Khakifirouz Arboviruses and Viral Hemorrhagic Fevers Laboratory (National Reference Lab), Pasteur Institute of Iran, Tehran, Iran.
  • Martin H Groschup Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany.
  • Matthias Niedrig Robert Koch Institute, Berlin, Germany.
Keywords:
Recombination, Phylogenetic, Diversity, Crimean Congo Hemorrhagic Fever, Iran

Abstract

Background: Crimean-Congo Hemorrhagic Fever Virus (CCHFV) belongs to genus Nairovirus and family Bunyaviridae. The main aim of this study was to investigate the extent of recombination in S-segment genome of CCHFV in Iran.Methods: Samples were isolated from Iranian patients and those available in GenBank, and analyzed by phyloge­netic and bootscan methods.Results: Through comparison of the phylogenetic trees based on full length sequences and partial fragments in the S-segment genome of CCHFV, genetic switch was evident, due to recombination event. Moreover, evidence of multi­ple recombination events was detected in query isolates when bootscan analysis was used by SimPlot software.Conclusion: Switch of different genomic regions between different strains by recombination could contribute to CCHFV diversification and evolution. The occurrence of recombination in CCHFV has a critical impact on epidemi­ological investigations and vaccine design. 

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Published
2016-02-06
How to Cite
1.
Chinikar S, Shah-Hosseini N, Bouzari S, Shokrgozar M, Mostafavi E, Jalali T, Khakifirouz S, Groschup M, Niedrig M. Assessment of Recombination in the S-segment Genome of Crimean-Congo Hemorrhagic Fever Virus in Iran. J Arthropod Borne Dis. 10(1):12–23.
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Original Article